FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences400035
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT18670.466709162948242No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT15830.3957153749046959No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13200.32997112752634145No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG12330.30822303048483257No Hit
GACCCATTTATGCCTACAGCCTCCTAGTACAAAAATCATTAACCTAGTCT11680.2919744522354294No Hit
ATTTATAGGAAGTTTCCGAAAGCATTGTTTGATTTTATGTACAATATGTT10400.25997725199045085No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT10010.25022810504080895No Hit
CATTTAAACCCTACTAAAACTAAACGGTGGGGATACTCCCTTAACTTCAT9740.24347869561413377No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA9250.2312297673953529No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT9180.22947992050695565No Hit
GTGCTGGTGTGTGCGGCGCCTGTTGGTGAACTATTGCAGAGCCTCCCAGC8860.22148062044571099No Hit
ACCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACACAGTCTTTG8610.21523116727286362No Hit
GACCAATTTATGCCTACAGCCTCCTAGTACAAAAATCATTAACCTAGTCT8480.21198145162298299No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC8250.2062319547039634No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG7770.19423300461209644No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA7490.18723361705850738No Hit
GCTTACTACTGCTTCCCTGGATGCTGGATCTTCCAAATTACTTCCCACCC6880.17198495131675978No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6860.171484995062932No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6850.1712350169360181No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAAATC6690.16723536690539578No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT6550.16373567312860124No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGCGAGAGGACAACAGAGT6480.161985826240204No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA6280.1569862637019261No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6220.15548639494044272No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTTGGGGGAGGAGACTA6210.15523641681352882No Hit
ATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAAGT6190.15473646055970103No Hit
AGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGT6050.15123676678290648No Hit
GTGCAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAACA6020.15048683240216482No Hit
GGAACATATTGTACATAAAATCAAACAATGCTTTCGGAAACTTCCTATAA5950.14873698551376754No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC5720.14298748859474797No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA5630.1407376854525229No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCAGCTCACCATACAG5600.1399877510717812No Hit
GGTCTATTTATAGGAAGTTTCCGAAAGCATTGTTTGATTTTATGTACAAT5490.13723799167572837No Hit
CCATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATC5330.13323834164510606No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5310.13273838539127827No Hit
GGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG5140.12848875723374203No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC5050.126238954091517No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5040.1259889759646031No Hit
GCCTTAGAGTCTCCGGAACATTGTTCAGCTCACCATACAGCACTAAGGCA4920.12298923844163635No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGT4890.12223930406089467No Hit
AGTTAAGGGAGTATCCCCACCGTTTAGTTTTAGTAGGGTTTAAATGAATA4860.12148936968015298No Hit
GTTTAAATGAATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA4840.1209894134263252No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG4770.11923956653792794No Hit
GTGTCGATAAGATAGGGGCATTTGGTGGTCTGTAAGGTGCAGGAGTGCGA4760.11898958841101404No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT4750.11873961028410014No Hit
GCTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGCG4740.11848963215718625No Hit
GTTAAGGGAGTATCCCCACCGTTTAGTTTTAGTAGGGTTTAAATGAATAC4710.11773969777644457No Hit
CCTGCACCTTACAGACCACCAAATGCCCCTATCTTATCGACACTTCCGGA4680.11698976339570288No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4660.11648980714187508No Hit
TCCATAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG4580.11448998212656393No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4560.11399002587273613No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4560.11399002587273613No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4530.11324009149199445No Hit
CTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTG4480.11199020085742498No Hit
CATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAGGGAGC4460.11149024460359719No Hit
GTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACCGC4430.11074031022285549No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC4430.11074031022285549No Hit
GCTTGTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGT4340.10849050708063046No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC4290.10724061644606098No Hit
CCTGCACCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGT4290.10724061644606098No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGAACGTCCTTTGTCTA4260.10649068206531928No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4240.10599072581149149No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCAGCTCACCA4090.10224105390778307No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG4080.10199107578086919No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG4060.10149111952704139No Hit
GCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGGCAAAGACCTGACC4040.10099116327321359No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTATAGGAAGTTTCCGAAAGC4040.10099116327321359No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)