FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences571097
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN58861.030648033521451No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT17130.2999490454336129No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT12850.22500555947588588No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC11830.207145195999979No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT10740.1880591213051373No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA10710.1875338164970224No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA10530.18438198764833294No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA10440.18280607322398823No Hit
CATGTATACAAGCCAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC9380.16424530333726145No Hit
CGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTTCCT9240.16179388089939187No Hit
ATATTAACGTTAACATAGCCGACTACTAATTCCCTGGATGCTGGATCTTC9010.15776654403717758No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT8870.155315121599308No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTCTACAATATGTT8810.1542645119830782No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT8790.15391430877766824No Hit
TCTACGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG8440.14778575268299432No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA8050.14095679017750049No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGACACCCGTG7960.13938087575315578No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA7850.1374547581234011No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7660.13412782767200668No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7590.1329021164530719No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC6650.11644256579880474No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC6650.11644256579880474No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA6640.11626746419609979No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG6590.11539195618257493No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC6520.11416624496364015No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTCT6250.10943850169060597No Hit
GGGCTGTACCGCCCGCTTCTCCGCCTGTTGTACCGACCGACCACGGGGCG6220.10891319688249106No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGGTTA6160.10786258726626125No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA6130.10733728245814633No Hit
GTGCTGGTGAACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC6120.10716218085544138No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCCAAACAG5940.1040103520067519No Hit
ATGTTAACGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCAC5850.1024344375824072No Hit
GGGCTATACCGCCCGCTTCTCCGCCTGTTGTACCGACCGACCACGGGGCG5780.10120872636347242No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA5740.10050831995265253No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)