FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524475
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGCTTGGAAGTAATGATTAACTATGTGTTCAGG39900.7607607607607607No Hit
TTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAATTAACGAAAAA37570.7163353830020497No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAACTTTAATCTCCTTTTTTCG29310.5588445588445589No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22190.4230897564230897No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT18350.34987368320701656No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA18170.3464416797750131No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAATTAACG16880.3218456551789885No Hit
ATCCTAACCATACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT15330.2922922922922923No Hit
CTCTTACAATTAACGAAAAAAGGAGATTAAAGTTGATTATGCCTGCTAGG15310.2919109585776253No Hit
CATTTAAACCCTACTAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT14940.2848562848562849No Hit
CTATTAATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTG14760.28142428142428144No Hit
TCCACAGAGTATGTAAATAATGCCTAGCTTGGAAGTAATGATTAACTATG14410.2747509414176081No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT14050.2678869345536012No Hit
GGATAGAACCTAGCAGGCATAATCAACTTTAATCTCCTTTTTTCGTTAAT13510.25759092425759095No Hit
GAGTATGTAAATAATGCCTAGCTTGGAAGTAATGATTAACTATGTGTTCA13150.25072691739358405No Hit
GTTAGTATACCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC13010.24805758139091472No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA12350.23547356880690212No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGCTT11980.22841889508556176No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT11600.22117355450688783No Hit
GTGTAAAGGGAGCGGCAAAGCCTAGAAGCCCCACAATTCGCTGACATACT11380.21697888364555032No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11090.2114495447828781No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAATTAACGAAAAA10660.20325086991753658No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC10540.2009628676295343No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA10360.19753086419753088No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGC10340.19714953048286382No Hit
ACCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG10130.1931455264788598No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT9960.18990418990418992No Hit
GATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC9870.1881881881881882No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC9790.18666285332952No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC9780.18647218647218647No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA9760.18609085275751944No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT9690.18475618475618474No Hit
ATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAATTAACGAAAA9310.17751084417751084No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC9300.17732017732017732No Hit
TATCAATAGATGTCAACAATATGTGGGCCCTCTTACAATTAACGAAAAAA9270.17674817674817675No Hit
CTATTGATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTG9260.1765575098908432No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9040.1723628390295057No Hit
GAATTAGTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACA8490.16187616187616188No Hit
ATCTCAATGTTAGTATACCTTGGACTCATAAGGTGGGAAACTTTACTGGG8190.15615615615615616No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG8160.1555841555841556No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAAT8030.15310548643881977No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC7990.15234281900948568No Hit
ATTAATAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAAC7970.15196148529481862No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAA7900.15062681729348396No Hit
GGTAAGGTTAGGATAGAACCTAGCAGGCATAATCAACTTTAATCTCCTTT7450.1420468087134754No Hit
TTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC7080.134992134992135No Hit
TTATTATCCTGAACACATAGTTAATCATTACTTCCAAGCTAGGCATTATT7070.13480146813480148No Hit
AACCATACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTATTATCC6870.13098813098813097No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCT6770.12908146241479573No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6690.12755612755612755No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA6470.12336145669479004No Hit
ATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGCTTGGA6430.12259878926545595No Hit
GCTTGGAAGTAATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCC6370.12145478812145478No Hit
GCCTTCCACAGAGTATGTAAATAATGCCTAGCTTGGAAGTAATGATTAAC6290.11992945326278659No Hit
CTTACAATTAACGAAAAAAGGAGATTAAAGTTGATTATGCCTGCTAGGTT6190.11802278468945135No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTAGTTAATCATTACTT6090.11611611611611612No Hit
GTTTCTCTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATG6060.11554411554411555No Hit
CAATAGATGTCAACAATATGTGGGCCCTCTTACAATTAACGAAAAAAGGA6060.11554411554411555No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6010.11459078125744793No Hit
ATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGG6010.11459078125744793No Hit
CAATATGTTCTTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATG5980.11401878068544737No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5920.1128747795414462No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT5910.11268411268411267No Hit
TTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA5890.11230277896944563No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC5870.11192144525477858No Hit
TAGTATACCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTT5870.11192144525477858No Hit
ATTGATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCC5830.11115877782544449No Hit
ATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCCAGTC5820.11096811096811096No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA5810.11077744411077745No Hit
GAATTAATAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACA5800.11058677725344392No Hit
ATGTAAATAATGCCTAGCTTGGAAGTAATGATTAACTATGTGTTCAGGAT5770.11001477668144334No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5760.10982410982410983No Hit
AACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTATTATCC5750.1096334429667763No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG5720.10906144239477572No Hit
AAATAATGCCTAGCTTGGAAGTAATGATTAACTATGTGTTCAGGATAATA5670.10810810810810811No Hit
GTACAAAACCTTCGGACGGAAATTGCACTTGTATTCCCATCCCATCATCT5650.10772677439344107No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGCTTGGAAGTAATGATTAACTA5620.1071547738214405No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC5530.10543877210543878No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA5490.10467610467610469No Hit
GATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCCAGT5470.10429477096143763No Hit
GCCTAGAAGCCCCACAATTCGCTGACATACTTTCCAATCAATGGGTCTAT5410.10315076981743648No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTATGGTT5400.10296010296010295No Hit
ATCCTGAACACATAGTTAATCATTACTTCCAAGCTAGGCATTATTTACAT5380.10257876924543591No Hit
GCCTAGCAGCCATGGAAAAGAGGTGTATTTCCGAGAGAGGACAACAGAGT5350.10200676867343533No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAGTAAT5340.10181610181610182No Hit
GTTCTATCCTAACCATACCAAATATTTGCCCTTAGATAAAGGCATTAAAC5330.10162543495876829No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTAGTAGGGTTTAAATGTATA5290.1008627675294342No Hit
CTATTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCCAGGTG5280.10067210067210067No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)