FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524475
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGCTTGGAAGTAATGATTAACTATGTGTTCAGG41310.7876447876447876No Hit
TTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAATTAACGAAAAA36190.6900233566900233No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAACTTTAATCTCCTTTTTTCG29730.5668525668525669No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22150.42232708899375565No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA18950.36131369464702795No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT16960.32337099003765674No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAATTAACG15830.3018256351589685No Hit
CTCTTACAATTAACGAAAAAAGGAGATTAAAGTTGATTATGCCTGCTAGG15780.3008723008723009No Hit
CATTTAAACCCTACTAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT13670.2606415939749273No Hit
GGATAGAACCTAGCAGGCATAATCAACTTTAATCTCCTTTTTTCGTTAAT13560.25854425854425855No Hit
GTGTAAAGGGAGCGGCAAAGCCTAGAAGCCCCACAATTCGCTGACATACT13500.2574002574002574No Hit
GTTAGTATACCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC13470.25682825682825683No Hit
CTATTAATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTG13360.2547309213975881No Hit
GAGTATGTAAATAATGCCTAGCTTGGAAGTAATGATTAACTATGTGTTCA13320.25396825396825395No Hit
TCCACAGAGTATGTAAATAATGCCTAGCTTGGAAGTAATGATTAACTATG13150.25072691739358405No Hit
ATCCTAACCATACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT12910.24615091281757948No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT12660.24138424138424136No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT12100.23070689737356406No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGCTT11870.22632155965489298No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGC11690.22288955622288956No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA11200.2135468802135469No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10930.20839887506554172No Hit
ACCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG10890.20763620763620763No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC10520.20058153391486724No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC10400.19829353162686497No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA10350.1973401973401973No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT10330.19695886362553028No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAATTAACGAAAAA9900.18876018876018877No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC9750.18590018590018592No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9680.18456551789885123No Hit
CTATTGATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTG9380.1788455121788455No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC9130.17407884074550742No Hit
GATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC9110.17369750703084036No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG8910.16988416988416988No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA8790.16759616759616758No Hit
ATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAATTAACGAAAA8750.1668335001668335No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT8670.1653081653081653No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAA8440.16092282758949425No Hit
GAATTAGTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACA8280.15787215787215786No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC8250.15730015730015728No Hit
TATCAATAGATGTCAACAATATGTGGGCCCTCTTACAATTAACGAAAAAA8210.1565374898708232No Hit
ATCTCAATGTTAGTATACCTTGGACTCATAAGGTGGGAAACTTTACTGGG7840.1494828161494828No Hit
GCCTTCCACAGAGTATGTAAATAATGCCTAGCTTGGAAGTAATGATTAAC7650.14586014586014587No Hit
TTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC7390.14090280756947424No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCT6970.13289479956146624No Hit
GGTAAGGTTAGGATAGAACCTAGCAGGCATAATCAACTTTAATCTCCTTT6960.1327041327041327No Hit
GTTTCTCTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATG6910.1317507984174651No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAAT6880.1311787978454645No Hit
GCCTAGAAGCCCCACAATTCGCTGACATACTTTCCAATCAATGGGTCTAT6850.13060679727346394No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGCTTGGAAGTAATGATTAACTA6800.1296534629867963No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA6690.12755612755612755No Hit
TTATTATCCTGAACACATAGTTAATCATTACTTCCAAGCTAGGCATTATT6680.12736546069879404No Hit
CAATAGATGTCAACAATATGTGGGCCCTCTTACAATTAACGAAAAAAGGA6550.12488679155345822No Hit
ATTAATAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAAC6470.12336145669479004No Hit
GCTTGGAAGTAATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCC6410.12221745555078888No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTAGTTAATCATTACTT6410.12221745555078888No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG6360.12126412126412126No Hit
CTTACAATTAACGAAAAAAGGAGATTAAAGTTGATTATGCCTGCTAGGTT6330.12069212069212068No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6230.11878545211878545No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6180.11783211783211783No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6110.11649744983078317No Hit
ATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGCTTGGA6050.11535344868678203No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA6040.1151627818294485No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC6020.11478144811478146No Hit
GTTTGATGCTGTAGCTCTTGTTCCCAAGAATATGGTGACCCACAAAATGA6000.11440011440011441No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT5950.11344678011344679No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA5940.11325611325611325No Hit
TTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA5900.11249344582677916No Hit
AACCATACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTATTATCC5850.11154011154011154No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5710.1088707755374422No Hit
CAATATGTTCTTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATG5700.10868010868010868No Hit
CAATATGTGGGCCCTCTTACAATTAACGAAAAAAGGAGATTAAAGTTGAT5690.10848944182277516No Hit
ATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCCAGTC5670.10810810810810811No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5660.10791744125077458No Hit
ATATGTGGGCCCTCTTACAATTAACGAAAAAAGGAGATTAAAGTTGATTA5640.10753610753610754No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTAGTAGGGTTTAAATGTATA5640.10753610753610754No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAGTAAT5550.10582010582010583No Hit
AAATAATGCCTAGCTTGGAAGTAATGATTAACTATGTGTTCAGGATAATA5470.10429477096143763No Hit
CTATTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCCAGGTG5380.10257876924543591No Hit
TTAGTATACCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCT5380.10257876924543591No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT5360.10219743553076886No Hit
GATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCCAGT5310.10124410124410124No Hit
GTTCTATCCTAACCATACCAAATATTTGCCCTTAGATAAAGGCATTAAAC5290.1008627675294342No Hit
ATGTGGGCCCTCTTACAATTAACGAAAAAAGGAGATTAAAGTTGATTATG5260.10029076695743362No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)