FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences283772
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA22710.8002903739621949No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT16530.5825099023159438No Hit
GGTCAATGTCCATGCCCCAAAGCCACCCAAGGCACAGCTTGGAGGCTTGA14170.4993445442115501No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC11160.3932734730699294No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11090.3908067039736126No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTC10480.369310573277138No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA10040.3538051675288612No Hit
GCCTAATCATCTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGC8690.30623176352846654No Hit
CACCAGCACCATGCAACTTTTTCACCTCTGCCTAATCATCTCATGTTCAT8310.2928407312913184No Hit
CCCCAAAGCCACCCAAGGCACAGCTTGGAGGCTTGAACAGTGGGACATGA7570.2667634579873983No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTCGGGGGAGGAGATTA6720.23680983324640908No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCACGAA6700.2361050420760329No Hit
CCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGACCCGTAT6500.2290571303722707No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG6460.22764754803151827No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6420.22623796569076582No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT6380.22482838335001337No Hit
CCCTTTACACAATGTGGGTACCCAGCATTAATGCCTTTGTATGCATGTAT6210.21883765840181557No Hit
TCTTTATACGGGTCAATGTCCATGCCCCAAAGCCACCCAAGGCACAGCTT6210.21883765840181557No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGACCCACAATTCTTTGACACACT6100.21496130696474636No Hit
GTCGTTGACATTGCAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG6020.21214214228324146No Hit
GCCTAGTCATCTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGC6000.21143735111286527No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5870.20685620850541983No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC5760.20297985706835062No Hit
CACCAGCACCATGCAACTTTTTCACCTCTGCCTAGTCATCTCATGTTCAT5340.1881792424904501No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5320.18747445132007387No Hit
CCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAAGAGTCCTCTTATGT5270.18571247339413333No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGGCCGACCACG5200.18324570429781656No Hit
CTCTTGGACTCTCTGCAATGTCAACGACCGACCTTGAGGCATACTTCAAA5170.18218851754225224No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5160.18183612195706414No Hit
GTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG5040.17760737493480683No Hit
GAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGCGGGCGT5010.1765501881792425No Hit
GGTGAAAAAGTTGCATGGTGCTGGTGAACAGACCAATTTATGCCTACAGC4940.17408341908292574No Hit
GGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGG4830.17020706764585652No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4800.1691498808902922No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACC4730.16668311179397544No Hit
GTACTCCTCCCCCGACTCCTCCCAGTCTTTAAATACACAGTCTTTGAAGT4690.165273529453223No Hit
GTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTGTATTTAAAGACT4680.1649211338680349No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG4660.16421634269765867No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCGCCAGGTCTTGCCC4650.16386394711247057No Hit
GTCCATGCCCCAAAGCCACCCAAGGCACAGCTTGGAGGCTTGAACAGTGG4530.15963520009021326No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT4530.15963520009021326No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4520.15928280450502516No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG4500.15857801333464894No Hit
GGCATAAATTGGTCTGTTCACCAGCACCATGCAACTTTTTCACCTCTGCC4440.1564636398235203No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA4410.15540645306795597No Hit
CGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGGCAGACGAAGAAGG4340.1529396839716392No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGG4250.14976812370494622No Hit
ATTTATAGGAAGTTTCCGAAAACATTGCTTGATTTTTTGTACAATATGTT4240.14941572811975812No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4200.1480061457790057No Hit
ACCTTGAGGCATACTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTCG4130.14553937668268893No Hit
GGCTTGAACAGTGGGACATGAACATGAGATGATTAGGCAGAGGTGAAAAA4080.1437773987567484No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4060.14307260758637216No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG3950.13919625614930298No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAA3930.13849146497892675No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC3900.13743427822336243No Hit
GTCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCGACGGG3870.13637709146779808No Hit
CTCCACAGAAGCTCCAAATTCTTTATACGGGTCAATGTCCATGCCCCAAA3860.13602469588260999No Hit
GCTTGGAGGCTTGAACAGTGGGACATGAACATGAGATGATTAGGCAGAGG3860.13602469588260999No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCACGAATAGAAGGAAA3840.13531990471223376No Hit
GTACTGGGAGGCTGTAGGCATAAATTGGTCTGTTCACCAGCACCATGCAA3820.13461511354185754No Hit
CTCTTATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTC3750.13214834444554077No Hit
CTACTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCG3740.13179594886035267No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC3710.13073876210478835No Hit
GTTCACCAGCACCATGCAACTTTTTCACCTCTGCCTAATCATCTCATGTT3710.13073876210478835No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTCGGGGGAGGAGTACT3710.13073876210478835No Hit
ACATTGCAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACC3690.13003397093441213No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG3650.12862438859365968No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTATTACCAATTTTCTT3630.12791959742328346No Hit
TTCTTTATACGGGTCAATGTCCATGCCCCAAAGCCACCCAAGGCACAGCT3630.12791959742328346No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTACCCACATTGTGTAAAG3630.12791959742328346No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT3500.12333845481583806No Hit
CCCAAGGCACAGCTTGGAGGCTTGAACAGTGGGACATGAACATGAGATGA3490.12298605923064995No Hit
GGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGA3470.12228126806027373No Hit
CGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGGCA3420.12051929013433318No Hit
GTATGGTGAGGTGAACAATGTTCCGGAGACTCTAAGGCCTCCCGATACAG3380.11910970779358077No Hit
GAGCAGAGGCGGTGTCGAGGAGATCACGAATAGAAGGAAAGAAGTCAGAA3370.11875731220839264No Hit
TCTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG3320.1169953342824521No Hit
CCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCACCT3310.116642938697264No Hit
CTGCAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTGTATTT3300.1162905431120759No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC3290.11593814752688777No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA3290.11593814752688777No Hit
GCACAGCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAAC3260.11488096077132345No Hit
CTACCGTCCCCTTCTTCGTCTGCCGTTCCGGCCGACCACGGGGCGCACCT3250.11452856518613536No Hit
CTTTATACGGGTCAATGTCCATGCCCCAAAGCCACCCAAGGCACAGCTTG3240.11417616960094723No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG3240.11417616960094723No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA3220.11347137843057103No Hit
CCTAATCATCTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGCC3200.11276658726019481No Hit
GGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGGCCGACCACGGGGCG3180.11206179608981859No Hit
CTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATT3140.11065221374906617No Hit
CTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC3140.11065221374906617No Hit
GTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTC3140.11065221374906617No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACTCCTCCC3140.11065221374906617No Hit
TGATTAGGCAGAGGTGAAAAAGTTGCATGGTGCTGGTGAACAGACCAATT3070.1081854446527494No Hit
ATACAGAGCAGAGGCGGTGTCGAGGAGATCACGAATAGAAGGAAAGAAGT3050.10748065348237318No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG3020.10642346672680884No Hit
GTAGAGTCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG3010.10607107114162073No Hit
CTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT3000.10571867555643263No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG2990.10536627997124452No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT2990.10536627997124452No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC2980.10501388438605641No Hit
GACAAAGGACGTCCCGCGCAGGATCCAGTTGGCAGCACAGCCTAGCAGCC2960.1043090932156802No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG2960.1043090932156802No Hit
TCTCGGGGCCGTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCG2940.10360430204530398No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA2910.10254711528973966No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA2890.10184232411936343No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)