FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489325

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences283772
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA22230.7833753858731658No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT16180.57017605683436No Hit
GGTCAATGTCCATGCCCCAAAGCCACCCAAGGCACAGCTTGGAGGCTTGA13220.46586696361867974No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12830.4521235357963435No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA10020.353100376358485No Hit
GCCTAATCATCTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGC9670.3407665308769012No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTC9580.33759497061020816No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC8770.3090509282099714No Hit
CACCAGCACCATGCAACTTTTTCACCTCTGCCTAATCATCTCATGTTCAT8580.3023554120913973No Hit
CCCCAAAGCCACCCAAGGCACAGCTTGGAGGCTTGAACAGTGGGACATGA8050.28367844607642756No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTCGGGGGAGGAGATTA7440.2621823153799529No Hit
CCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGACCCGTAT7300.25724877718731937No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCACGAA6770.2385718111723496No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6510.22940952595745878No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA6440.22694275686114207No Hit
GCCTAGTCATCTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGC6400.22553317452038962No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG6270.2209520319129442No Hit
CCCTTTACACAATGTGGGTACCCAGCATTAATGCCTTTGTATGCATGTAT6260.2205996363277561No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGACCCACAATTCTTTGACACACT6190.21813286723143932No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6130.21601849372031068No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT6130.21601849372031068No Hit
TCTTTATACGGGTCAATGTCCATGCCCCAAAGCCACCCAAGGCACAGCTT5890.20756099967579605No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGGCCGACCACG5870.20685620850541983No Hit
GTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG5640.19875111004609333No Hit
CTCTTGGACTCTCTGCAATGTCAACGACCGACCTTGAGGCATACTTCAAA5380.1895888248312025No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5350.1885316380756382No Hit
GTCGTTGACATTGCAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG5240.184655286638569No Hit
CACCAGCACCATGCAACTTTTTCACCTCTGCCTAGTCATCTCATGTTCAT5220.18395049546819278No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG5120.1804265396163117No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4920.17337862791254952No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG4910.1730262323273614No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG4850.17091185881623275No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT4790.16879748530510408No Hit
GTCCATGCCCCAAAGCCACCCAAGGCACAGCTTGGAGGCTTGAACAGTGG4770.16809269413472788No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4750.16738790296435166No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACC4690.165273529453223No Hit
GTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTGTATTTAAAGACT4590.16174957360134193No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4570.1610447824309657No Hit
GGCATAAATTGGTCTGTTCACCAGCACCATGCAACTTTTTCACCTCTGCC4470.15752082657908462No Hit
GGTGAAAAAGTTGCATGGTGCTGGTGAACAGACCAATTTATGCCTACAGC4450.1568160354087084No Hit
GTACTGGGAGGCTGTAGGCATAAATTGGTCTGTTCACCAGCACCATGCAA4410.15540645306795597No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCGCCAGGTCTTGCCC4400.15505405748276785No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG4350.1532920795568273No Hit
CCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAAGAGTCCTCTTATGT4250.14976812370494622No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG4220.1487109369493819No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4180.14730135460862948No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC4160.14659656343825325No Hit
GTACTCCTCCCCCGACTCCTCCCAGTCTTTAAATACACAGTCTTTGAAGT4160.14659656343825325No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4090.14412979434193648No Hit
CTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAGACC4050.14272021200118407No Hit
GTTCACCAGCACCATGCAACTTTTTCACCTCTGCCTAATCATCTCATGTT4050.14272021200118407No Hit
GTCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCGACGGG4050.14272021200118407No Hit
GGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGG3920.13813906939373866No Hit
GAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGCGGGCGT3920.13813906939373866No Hit
ACCTTGAGGCATACTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTCG3840.13531990471223376No Hit
ATTTATAGGAAGTTTCCGAAAACATTGCTTGATTTTTTGTACAATATGTT3820.13461511354185754No Hit
CTCCACAGAAGCTCCAAATTCTTTATACGGGTCAATGTCCATGCCCCAAA3800.13391032237148132No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC3700.13038636651960023No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTCGGGGGAGGAGTACT3660.1289767841788478No Hit
GGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGGCCGACCACGGGGCG3640.12827199300847159No Hit
GGCTTGAACAGTGGGACATGAACATGAGATGATTAGGCAGAGGTGAAAAA3620.12756720183809536No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACTCCTCCC3580.12615761949734294No Hit
CGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGGCAGACGAAGAAGG3570.12580522391215482No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTACCCACATTGTGTAAAG3550.1251004327417786No Hit
GTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTC3540.1247480371565905No Hit
CCCAAGGCACAGCTTGGAGGCTTGAACAGTGGGACATGAACATGAGATGA3510.12369085040102618No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT3490.12298605923064995No Hit
CTACTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCG3470.12228126806027373No Hit
TTCTTTATACGGGTCAATGTCCATGCCCCAAAGCCACCCAAGGCACAGCT3460.12192887247508563No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCACGAATAGAAGGAAA3430.12087168571952131No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA3400.11981449896395699No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG3400.11981449896395699No Hit
CTACCGTCCCCTTCTTCGTCTGCCGTTCCGGCCGACCACGGGGCGCACCT3390.11946210337876886No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA3380.11910970779358077No Hit
CTCTTATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTC3380.11910970779358077No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGG3340.11770012545282832No Hit
CTTTATACGGGTCAATGTCCATGCCCCAAAGCCACCCAAGGCACAGCTTG3340.11770012545282832No Hit
CTGCAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTGTATTT3320.1169953342824521No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAA3310.116642938697264No Hit
GCTTGGAGGCTTGAACAGTGGGACATGAACATGAGATGATTAGGCAGAGG3270.11523335635651158No Hit
CTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC3250.11452856518613536No Hit
TCTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG3190.11241419167500669No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG3190.11241419167500669No Hit
ACATTGCAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACC3180.11206179608981859No Hit
GCCTTGGGTGGCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGA3170.11170940050463049No Hit
ACCCAAGGCACAGCTTGGAGGCTTGAACAGTGGGACATGAACATGAGATG3150.11100460933425427No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC3140.11065221374906617No Hit
TCTCGGGGCCGTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCG3110.10959502699350182No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG3090.10889023582312561No Hit
GCACAGCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAAC3070.1081854446527494No Hit
GTATGGTGAGGTGAACAATGTTCCGGAGACTCTAAGGCCTCCCGATACAG3060.10783304906756128No Hit
TTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC3050.10748065348237318No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA3050.10748065348237318No Hit
CTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATT3030.10677586231199696No Hit
GGTTGGGGCTTGGCCATGGGCCATCAGCGCATGCGTGGAACCTTTGTGGC3030.10677586231199696No Hit
CCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGAC2960.1043090932156802No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA2900.10219471970455153No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT2900.10219471970455153No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTATTACCAATTTTCTT2890.10184232411936343No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC2850.10043274177861099No Hit
CCACGGGGCGCACCTCTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCAT2850.10043274177861099No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)