FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463105
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN45860.9902721844937974No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA17250.3724857213806804No Hit
CAATTACATATCCCATGAAGTTAAGGGAATAACCCCATCTTTTTGTTTTG15150.32713963356042364No Hit
TTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACC15130.3267077660573736No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT11750.2537221580419127No Hit
TTGTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAAC11390.2459485429870116No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA10950.23644745791991015No Hit
CAATGACATATCCCATGAAGTTAAGGGAATAACCCCATCTTTTTGTTTTG8890.19196510510575354No Hit
TATCCACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTTGC8570.18505522505695254No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGTTATTCCCTTAACTTCAT8170.17641787499595124No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT8140.17577007374137615No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT8050.17382666997765084No Hit
CCTAGTACAAAGACCTTTAACCTGATCTCCTCCCCCAACTCCTCCCACTC7690.1660530549227497No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG7080.15288109607972275No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG6880.1485624210492221No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC6720.14510748102482157No Hit
ATGTAATTGGGTGTTGGGGCACATTGCCACAAGAACATATTGTACAAAAA6440.1390613359821207No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA6300.13603826346077025No Hit
GCTTTACTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT6190.13366299219399488No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTTCCTCCCCC6150.13279925718789476No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC5920.12783278090281902No Hit
CTCCAAAGGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA5870.12675311214519386No Hit
GCCGACCCGTGTACACTTCGCTTCACCTCTGCACGTCGCATGGAGACCAC5840.12610531089061874No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA5820.1256734433875687No Hit
CGGTCCGGTAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGT5720.12351410587231838No Hit
ATGTCATTGGGGGTTGGGGCACATTGCCACAAGAACATATTGTACAAAAA5450.11768389458114249No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT5410.11682015957504238No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT5410.11682015957504238No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC5380.11617235832046728No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5330.11509268956284213No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAGGACA5330.11509268956284213No Hit
CAATAGGTCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT5160.11142181578691658No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAGGTTCCGGTGAGCGTTC5090.10991027952624134No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT5010.10818280951404109No Hit
CCTGTAAACAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT4830.1042960019865905No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG4830.1042960019865905No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT4770.10300039947744033No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC4690.10127292946524005No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)