FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463105
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN45790.988760648233122No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA16850.3638483713196791No Hit
TTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACC15080.32562809729974845No Hit
CAATTACATATCCCATGAAGTTAAGGGAATAACCCCATCTTTTTGTTTTG13610.2938858358255687No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT12210.2636551106120642No Hit
TTGTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAAC11050.2386067954351605No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA10370.22392330033145832No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT8780.1895898338389782No Hit
TATCCACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTTGC8460.18267995379017718No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT8230.17771347750510144No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGTTATTCCCTTAACTTCAT7810.1686442599410501No Hit
CAATGACATATCCCATGAAGTTAAGGGAATAACCCCATCTTTTTGTTTTG7570.1634618499044493No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG7560.16324591615292428No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG7470.161302512389199No Hit
CCTAGTACAAAGACCTTTAACCTGATCTCCTCCCCCAACTCCTCCCACTC6920.14942615605532222No Hit
ATGTAATTGGGTGTTGGGGCACATTGCCACAAGAACATATTGTACAAAAA6890.14877835480074714No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC6750.1457552822793967No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTTCCTCCCCC6320.1364701309638203No Hit
CTCCAAAGGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA6230.134526727200095No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA5930.12804871465434403No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA5770.12459377462994353No Hit
GCCGACCCGTGTACACTTCGCTTCACCTCTGCACGTCGCATGGAGACCAC5710.12329817212079335No Hit
ATGTCATTGGGGGTTGGGGCACATTGCCACAAGAACATATTGTACAAAAA5600.120922900854018No Hit
GCTTTACTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT5590.12070696710249294No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAGGACA5550.11984323209639283No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC5510.1189794970902927No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT5300.11444488830826703No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT5300.11444488830826703No Hit
CCTGTAAACAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5210.11250148454454173No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT5210.11250148454454173No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAGGTTCCGGTGAGCGTTC5200.11228555079301669No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT5120.11055808078081644No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC5050.10904654452014123No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT5050.10904654452014123No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4800.10364820073201543No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC4770.10300039947744033No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT4770.10300039947744033No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT4760.10278446572591529No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG4740.10235259822286523No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG4660.10062512821066498No Hit
CAATAGGTCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT4660.10062512821066498No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC4650.10040919445913994No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)