FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences659755
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN75121.1386044819667909No Hit
GTTAATATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC23720.35952740032284713No Hit
GTATGTAAATAGTGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG19740.2992019764912733No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG17660.2676751218255261No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATATGTT17420.26403740782563223No Hit
GTGTAAAGGGAGCGGCAAACCCCAAAAGACCCACAATTCTTTGACATACT17160.26009655099241386No Hit
CCTTTATACCGCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA15160.22978226765996468No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT14870.22538669657675955No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG14140.21432198316041562No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC13960.21159369766049518No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATAACCCATGAA13320.20189312699411144No Hit
CCCTTAACTTCATGGGTTATGTAATTGGAAGTTGGGGTACCTTGCCACAG13160.1994679843275155No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA13040.1976491273275686No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT13010.19719441307758184No Hit
GGAACATATTGTACAAAAAATCAAACAATGTTTTCGGAAACTTCCTATAA11500.1743071291615827No Hit
GGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTAAGGCG11090.1680927010784306No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA10900.16521284416184795No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG10870.16475812991186123No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAAT9610.14566013141241824No Hit
GTATAAAGGGATTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC9480.14368970299580905No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT9430.14293184591249783No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT9390.14232556024584886No Hit
TCTTCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCGTCTATTCG9190.13929413191260392No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCAT9020.13671741782934574No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT8940.13550484649604777No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA8900.1348985608293988No Hit
CTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATAT8800.13338284666277633No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA8750.13262498957946509No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTC8190.12413699024637934No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAACTCAGACAAATATT8160.1236822759963926No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC8110.12292441891308137No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTATAGGAAGTTTCCGAAAAC8070.1223181332464324No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTA7950.12049927624648543No Hit
AGTCTGTACAACATCTTGAATCCCTTTATACCGCTATTACCAATTTTCTT7940.12034770482982318No Hit
ATGTAAATAGTGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGGAT7790.11807413357988951No Hit
CAATAGGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGT7570.11473956241332009No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT7200.109131419996817No Hit
ATGTTAATATGGGCCTAAAACTCAGACAAATATTGTGGTTTCACATTTCC7160.10852513433016803No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7040.10670627733022106No Hit
TCTTTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCGTCTATTCG6920.10488742033027412No Hit
GTTCTATCCTCGCCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAAC6860.10397799183030064No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA6840.10367484899697615No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6730.10200756341369145No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCGTTAAC6660.10094656349705572No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6620.10034027783040675No Hit
ACTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATA6620.10034027783040675No Hit
GTACAATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATAACCC6620.10034027783040675No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)