FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410670
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA19180.4670416636228602No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC15000.3652567755131858No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14800.3603866851730099No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13230.32215647600262987No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT11120.27077702291377503No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT10840.2639588964375289No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG10190.24813110283195752No Hit
ATCTTAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG8790.21404047045072688No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA8690.21160542528063897No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT8570.20868337107653348No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAGTATTTAGTAACATT8370.20381328073635763No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC8330.20283926266832247No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT8290.20186524460028732No Hit
GTTGACAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG8020.19529062264105No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT7910.19261207295395327No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT7840.19090754133489177No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC7690.1872549735797599No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG7570.1843329193756544No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT7350.17897582000146103No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAGTA7220.17581026128034677No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA7210.17556675676333797No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA6870.16728760318503907No Hit
CTTCAAAGACTGTGTGTTTAATGAGTGGGAGGAGTTGGGGGAGGAGGTTA6710.16339153091289843No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA6660.16217400832785447No Hit
ACTAAATACTTGCCCTTAGATAAAGGGATCAAACCGTATTATCCAGAGTA6420.15632989991964352No Hit
GTATTTAGTAACATTGGGATAAAACCTAGCAGGCATAATTAATTTGAGTC6340.1543818637835732No Hit
AGATAATGGGATGGGAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTAC6230.1517033140964765No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT6210.15121630506245892No Hit
CTATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGT6170.15024228699442374No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTTA6070.14780724182433583No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT6010.1463462147222831No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAGATTCCCGTGGGCGTTC5950.14488518762023037No Hit
CATACCTTCCAATCAATAGGCCTGTTAACAGGAAGTTTCCTAAAACACAT5690.1385540701780018No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG5650.13758005210996663No Hit
CTTTAGGGCATGGACATTGACACGTATAAAGAATTTGGAGCTTCTGTGGA5640.13733654759295782No Hit
ATTGTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAA5630.13709304307594905No Hit
ATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAA5560.13538851145688752No Hit
CATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT5530.13465799790586117No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA5520.13441449338885236No Hit
ATCTAAGGGCAAGTATTTAGTAACATTGGGATAAAACCTAGCAGGCATAA5460.13295346628679963No Hit
CAATAGGCCTGTTAACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT5460.13295346628679963No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA5440.13246645725278205No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA5310.12930089853166776No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG5260.1280833759466238No Hit
GTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGCAAGACCTTGGG5110.12443080819149195No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT5030.12248277205542163No Hit
TTTTTCGGGTCACCATATTCTTGGGAACAAGATCTACAGCATGGGAGGTT5010.12199576302140405No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT4980.12126524947037767No Hit
CACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAACCT4960.1207782404363601No Hit
GGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGA4960.1207782404363601No Hit
CTCTTATGCAAGACCTTGGGCAGATTCCCGTGGGCGTTCACGGTGGTCTC4820.11736917719823703No Hit
GGGTAAAGGTTCAGATACTGTTTACTGAGAAAGGCCTTGTAAGTTGGCGA4810.11712567268122823No Hit
CCCTTACAGTAAATGAAAACAGGAGACTCAAATTAATTATGCCTGCTAGG4780.11639515913020188No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT4750.1156646455791755No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG4700.11444712299413154No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA4700.11444712299413154No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG4700.11444712299413154No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4700.11444712299413154No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA4610.11225558234105243No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAGTATTTAGTA4600.11201207782404364No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4590.11176857330703485No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT4550.11079455523899967No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4490.10933352813694694No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG4470.10884651910292936No Hit
TCTGTCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT4450.10835951006891177No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC4420.10762899651788539No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT4420.10762899651788539No Hit
TCCTAGTACAAAGACCTTTAACCTAACCTCCTCCCCCAACTCCTCCCACT4370.10641147393284146No Hit
GCTTTAGGGCATGGACATTGACACGTATAAAGAATTTGGAGCTTCTGTGG4370.10641147393284146No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA4370.10641147393284146No Hit
AGATGATGGGATGGGAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTAC4260.10373292424574476No Hit
CCTTTAACCTAACCTCCTCCCCCAACTCCTCCCACTCATTAAACACACAG4220.1027589061777096No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA4210.1025154016607008No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4200.10227189714369202No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT4170.10154138359266564No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC4140.10081087004163929No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGC4110.1000803564906129No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)