FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410670
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA17740.4319770131735944No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC15660.38132807363576593No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14790.36014318065600115No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13010.31679937662843644No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT10780.26249786933547614No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT10420.25373170672315976No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG9850.23985194925365866No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA9030.21988457885893783No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG8520.20746584849148952No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT8410.20478729880439284No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAGTATTTAGTAACATT8390.20430028977037523No Hit
ATCTTAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG8270.20137823556626977No Hit
GTTGACAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG8030.19553412715805876No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT8030.19553412715805876No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC7520.18311539679061048No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA7300.17775829741641708No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC7220.17581026128034677No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA7170.17459273869530278No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT7090.17264470255923248No Hit
CTTCAAAGACTGTGTGTTTAATGAGTGGGAGGAGTTGGGGGAGGAGGTTA7060.1719141890082061No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT6870.16728760318503907No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT6530.1590084496067402No Hit
GTATTTAGTAACATTGGGATAAAACCTAGCAGGCATAATTAATTTGAGTC6480.15779092702169625No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6380.15535588185160834No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTTA6210.15121630506245892No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT6160.14999878247741494No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAGTA5970.14537219665424794No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA5900.1436676650351864No Hit
ACTAAATACTTGCCCTTAGATAAAGGGATCAAACCGTATTATCCAGAGTA5870.14293715148416003No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA5600.13636252952492267No Hit
AGATAATGGGATGGGAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTAC5570.13563201597389632No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC5500.13392748435483479No Hit
ATCTAAGGGCAAGTATTTAGTAACATTGGGATAAAACCTAGCAGGCATAA5380.1310054301507293No Hit
ATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAA5360.13051842111671172No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA5330.12978790756568534No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG5270.1283268804636326No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG5260.1280833759466238No Hit
CTATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGT5150.12540482625952712No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA5110.12443080819149195No Hit
CCCTTACAGTAAATGAAAACAGGAGACTCAAATTAATTATGCCTGCTAGG5090.12394379915747436No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT5030.12248277205542163No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT5010.12199576302140405No Hit
ATTGTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAA5000.12175225850439525No Hit
CTTTAGGGCATGGACATTGACACGTATAAAGAATTTGGAGCTTCTGTGGA4980.12126524947037767No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT4970.12102174495336888No Hit
TTTTTCGGGTCACCATATTCTTGGGAACAAGATCTACAGCATGGGAGGTT4950.12053473591935131No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG4920.11980422236832493No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAGATTCCCGTGGGCGTTC4890.11907370881729858No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA4860.1183431952662722No Hit
CATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT4830.1176126817152458No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA4790.11663866364721065No Hit
CAATAGGCCTGTTAACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT4760.1159081500961843No Hit
TCCTAGTACAAAGACCTTTAACCTAACCTCCTCCCCCAACTCCTCCCACT4730.11517763654515792No Hit
TCTGTCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT4730.11517763654515792No Hit
CCTTTAACCTAACCTCCTCCCCCAACTCCTCCCACTCATTAAACACACAG4660.11347310492609637No Hit
CATACCTTCCAATCAATAGGCCTGTTAACAGGAAGTTTCCTAAAACACAT4630.11274259137507002No Hit
CACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAACCT4630.11274259137507002No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG4610.11225558234105243No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT4570.11128156427301725No Hit
GCTTTAGGGCATGGACATTGACACGTATAAAGAATTTGGAGCTTCTGTGG4560.11103805975600847No Hit
GGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGA4510.10982053717096452No Hit
CTGTTGTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCCC4450.10835951006891177No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA4430.1078725010348942No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT4410.10738549200087662No Hit
GTATACATCATTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC4400.10714198748386781No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG4360.10616796941583266No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4350.10592446489882387No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC4350.10592446489882387No Hit
CTCTTATGCAAGACCTTGGGCAGATTCCCGTGGGCGTTCACGGTGGTCTC4330.1054374558648063No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC4300.10470694231377992No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC4240.10324591521172719No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA4170.10154138359266564No Hit
GTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGCAAGACCTTGGG4150.10105437455864806No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT4150.10105437455864806No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC4130.10056736552463048No Hit
GGGTAAAGGTTCAGATACTGTTTACTGAGAAAGGCCTTGTAAGTTGGCGA4120.1003238610076217No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAGTATTTAGTA4120.1003238610076217No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)