FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences447327
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAAAGGGAGCGGCAAAACCCAAGAGACCCACAATTCTTTGACATACT24880.5561926733686989No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG19300.43145171205851646No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG19190.4289926608498928No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT16110.36013922700843004No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG15890.3552211245911827No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC15260.3411374676690654No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT15080.3371135656913175No Hit
CTGTAGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACA14540.3250418597580741No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA14220.31788825624207795No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG14180.31699405580257844No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG12730.28457928987072095No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG12580.2812260382225978No Hit
CCTCCCGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12480.278990537123849No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC11640.26021232789435916No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA11040.24679932130186644No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG10530.2353982656982476No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT10220.22846821229212635No Hit
AGCGAGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA9970.22287945954525437No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG9770.21840845734775677No Hit
CATGTATACAAGCTAAGCAAGCTTTCACTTTCTCGCCAACCTACAAGGCA9680.21639650635888286No Hit
TTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC8890.19873604767876746No Hit
GTCCACAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA8570.19158244416277131No Hit
CTTCTGGACTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC8550.19113534394302156No Hit
CCCACGTGTCCTGGCCAAAATTTGCAGTCCCCAACCTCCAATCACTCACC8490.18979404328377228No Hit
CCCTTCACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT8470.18934694306402253No Hit
GCCACGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCGGTG8390.1875585421850235No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8240.1842052905369003No Hit
GTAGAGTTTCCTTGAGCAGGACTCGTGCAGGTCTTGCATGGTCCCGTGCT8130.18174623932827663No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGAGCATGGACATTGA8060.18018138855915247No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT7810.1745926358122805No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCAAGACCTTTAACCT7530.16833323273578388No Hit
GAACCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAAT7470.16699193207653462No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7390.16520353119753559No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTA7360.16453288086791096No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATA7320.16363868042841143No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC7220.16140317932966264No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAATGCCTTGTAGGTTGGCGA7090.15849702790128922No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG7070.15804992768153947No Hit
TGCCTGCTAGGTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAA6910.1544731259235414No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCAC6890.15402602570379162No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCGTTAAC6830.15268472504454236No Hit
CCTTTGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT6690.14955502350629404No Hit
ATACTACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG6640.14843727295691966No Hit
CTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGT6530.145978221748296No Hit
TAGCCGGCATGATCAATTTTAATCTCCTTTTTTCGTTAACTGTAAGAGGG6500.14530757141867134No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC6490.14508402130879647No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG6460.14441337097917184No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA6350.14195431977054818No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC6350.14195431977054818No Hit
CCTTTATACCGCTATTACCAATTTTCTTGTGTCTTTGGGTATACATTTAA6320.14128366944092355No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA6210.13882461823229986No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG6140.1372597674631757No Hit
ACACACAATAGCTTGCCTTAGTGCTGTATGGTGAGGTGAACAATGTTCCG6120.13681266724342594No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG6090.13614201691380132No Hit
CCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGC6070.13569491669405156No Hit
CTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTA5930.13256521515580325No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG5880.13144746460642887No Hit
AGTCTAGACTCTGTAGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5800.12965906372742983No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAAC5710.12764711273855592No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA5710.12764711273855592No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA5680.1269764624089313No Hit
GGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT5660.1265293621891815No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCA5630.12585871185955688No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5530.1236232107608081No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC5500.12295256043118345No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5460.12205835999168395No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5460.12205835999168395No Hit
AGCGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA5440.12161125977193418No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5310.11870510834356075No Hit
CTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCTTG5310.11870510834356075No Hit
GGAACATATTGTACACAAAATCAAACAATGTTTTCGGAAACTTCCTATAA5300.11848155823368588No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5240.11714025757443661No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT5170.11557540680531245No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT5160.11535185669543757No Hit
GTGCACACGGACCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAA5140.1149047564756878No Hit
GATCCATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGCAGCCGGTCTG5080.11356345581643854No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGACCGGC5030.11244570526706414No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTC4910.10976310394856557No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4900.10953955383869073No Hit
CTTATATAAGACCTTAGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCA4880.10909245361894096No Hit
TCGACAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCTCTGGGATTCT4850.1084218032893163No Hit
GTATGCATGTATACAAGCTAAGCAAGCTTTCACTTTCTCGCCAACCTACA4840.10819825317944143No Hit
CACACAATAGCTTGCCTTAGTGCTGTATGGTGAGGTGAACAATGTTCCGG4780.10685695252019216No Hit
CCTTTTCGAGGTTTGGAAGACCAACCTCCCATGCTGTAGCTCTTGTTCCC4740.10596275208069265No Hit
GTTAACATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTG4660.10417435120169362No Hit
CTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACA4660.10417435120169362No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4530.10126819977332019No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4500.10059754944369555No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)