FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217536
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGGTA20780.9552441894674903No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA15950.7332119741100324No Hit
AAGGTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACACAGTCTTT15310.7037915563401No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA13360.6141512209473374No Hit
GGTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACACAGTCTTTGA10600.4872756693145043No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA8790.4040710503089144No Hit
GCCTAATCATCTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGC8650.39763533392174166No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG8140.37419093851132684No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACC7230.3323587819947043No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7180.33006031185642837No Hit
CACACAGTAGCTTGCCTTAGTGCTGTATGGTGAGGTGAACAATGTTCCGG7140.3282215357458076No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG6820.31351132686084143No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6550.3010995881141512No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6260.2877684613121506No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGGTAATC6040.2776551927037364No Hit
GTCTGTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5870.2698403942335981No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5800.2666225360400118No Hit
ATTGACAACAGTACCAGCAGCGCCTCCTACTGCCTCCACCAATCGGCAGT5750.2643240659017358No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT5590.2569689614592527No Hit
CCCTTTACACAATGTGGTTACCCGGCATTAATGCCTTTGTATGCATGTAT5380.2473153868784937No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5230.24041997646366578No Hit
CCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGACCCGTAT5220.2399602824360106No Hit
CTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGT5210.23950058840835542No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT5100.23444395410414826No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC5060.23260517799352753No Hit
GTTTTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTG4740.21789496910856135No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT4720.21697558105325093No Hit
GACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACAC4700.21605619299794057No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG4680.21513680494263018No Hit
GTACAAAGGTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACACAG4680.21513680494263018No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG4550.20916078258311266No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC4470.20548323036187113No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG4340.19950720800235364No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG4340.19950720800235364No Hit
GAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCTTCCT4270.1962893498087673No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT4120.1893939393939394No Hit
ATGTAAGACCTTGGGCAAGACCTGTCGGGCGTTCACGGTGGTCTCCATGC4040.18571638717269787No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG3790.174224036481318No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGTCGGGCGTTC3680.16916740217711093No Hit
CAGAATAGCTTGCCTTAGTGCTGTATGGTGAGGTGAACAATGTTCCGGAG3650.16778832009414535No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT3560.1636510738452486No Hit
GTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG3540.1627316857899382No Hit
ATCCTAACAGTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT3480.15997352162400708No Hit
AAGACCAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACACAGTCTT3470.15951382759635185No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCACGAA3410.15675566343042072No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT3370.15491688731979994No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG3330.15307811120917916No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC3260.14986025301559283No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCA3210.14756178287731686No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC3200.14710208884966167No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT3160.14526331273904092No Hit
CTATTCTGTGTTGGGGTGAGGTGATGAATCTGGCCACCTGGGTGGGAAGT3130.1438842306560753No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTACTGTT3080.14158576051779936No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCACGAATAGAAGGAAA3070.14112606649014414No Hit
AAGGTAATCTCCTCCCCCAACTCCTCCCAGTCTGTAAA3060.14066637246248898No Hit
GACCAATTTATGCCTACAGCCTCCCAGTACAAAGGTAATCTCCTCCCCCA2960.13606943218593706No Hit
CTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATT2940.13515004413062665No Hit
AGCAAGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA2920.13423065607531628No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAA2910.13377096204766106No Hit
GTATACATGCATACAAAGGCATTAATGCCGGGTAACCACATTGTGTAAAG2900.1333112680200059No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATG2860.13147249190938512No Hit
GTCACCCTCAGGCCATGCAGTGGAACTCCAGCACATTCCACCAAGCTCTG2860.13147249190938512No Hit
ATTGAAAACAGTACCAGCAGCGCCTCCTACTGCCTCCACCAATCGGCAGT2850.13101279788172993No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG2810.12917402177110915No Hit
CCTTACCACAGGACCATATTGTACACAAAATCAAACAATGTTTTCGGAAA2800.12871432774345395No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT2800.12871432774345395No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA2790.12825463371579876No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA2780.12779493968814357No Hit
GGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAG2740.1259561635775228No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA2730.12549646954986762No Hit
GCTACTGTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAG2720.12503677552221243No Hit
TGGAAACCACCGTGAACGCCCGACAGGTCTTGCCCAAGGTCTTACATAAG2710.12457708149455722No Hit
GCACAGCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAAC2670.12273830538393644No Hit
ATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG2660.12227861135628126No Hit
TTCTATTCGTGATCTCCTCGACACCGCCTCTGCTCTGTATCGGGAGGCCT2660.12227861135628126No Hit
ATTTATGCCTACAGCCTCCCAGTACAAAGGTAATCTCCTCCCCCAACTCC2640.12135922330097086No Hit
GTATGGTGAGGTGAACAATGTTCCGGAGACTCTAAGGCCTCCCGATACAG2630.12089952927331568No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTG2540.11676228302441895No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC2530.11630258899676377No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC2510.11538320094145336No Hit
CATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGC2500.11492350691379817No Hit
CCCCCAACTCCTCCCAGTCTTTAAATACACAGTCTTTGAAGTATGCCTCA2490.11446381288614299No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC2480.11400411885848778No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA2460.11308473080317741No Hit
GGGTAAAGGTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA2450.11262503677552221No Hit
AAGTCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACACAGTCTT2440.112165342747867No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG2430.11170564872021183No Hit
CTGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTC2420.11124595469255663No Hit
GTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG2410.11078626066490145No Hit
ATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCA2400.11032656663724624No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA2370.10894748455428067No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCGACAGGTCTTGCCC2340.10756840247131509No Hit
GCTACTGTGTGTTGGGGTGAGGTGATGAATCTGGCCACCTGGGTGGGAAG2340.10756840247131509No Hit
GAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGTCGGGCGT2330.10710870844365991No Hit
ACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCTTGAGG2330.10710870844365991No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG2300.10572962636069433No Hit
GCCTTGGGTGGCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGA2300.10572962636069433No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC2290.10526993233303913No Hit
TATTGACAACAGTACCAGCAGCGCCTCCTACTGCCTCCACCAATCGGCAG2290.10526993233303913No Hit
CTCTTGGACTCTCAGCAATGTCAACGACCGACCTTGAGGCATACTTCAAA2260.10389085025007355No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGA2250.10343115622241837No Hit
ATATGGTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA2220.10205207413945278No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA2220.10205207413945278No Hit
TCTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG2210.1015923801117976No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)