FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217536
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGGTA15330.7047109443954105No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA13650.6274823477493381No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA10550.4849771991762283No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG8850.40682921447484555No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA8220.37786849073256845No Hit
GCCTAATCATCTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGC7680.353045013239188No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7660.3521256251838776No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7140.3282215357458076No Hit
CACACAGTAGCTTGCCTTAGTGCTGTATGGTGAGGTGAACAATGTTCCGG6700.3079949985289791No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6280.288687849367461No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG6270.28822815533980584No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6150.2827118270079435No Hit
AAGGTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACACAGTCTTT5840.26846131215063257No Hit
CCCTTTACACAATGTGGTTACCCGGCATTAATGCCTTTGTATGCATGTAT5720.26294498381877024No Hit
CCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGACCCGTAT5560.25558987937628713No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACC5310.24409752868490733No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5230.24041997646366578No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5080.2335245660488379No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA5060.23260517799352753No Hit
CTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGT5050.23214548396587234No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG4990.22938731979994118No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT4870.22387099146807884No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG4790.2201934392468373No Hit
GTTTTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTG4750.21835466313621654No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG4750.21835466313621654No Hit
ATTGACAACAGTACCAGCAGCGCCTCCTACTGCCTCCACCAATCGGCAGT4600.21145925272138866No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC4550.20916078258311266No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT4440.20410414827890555No Hit
GTCTGTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA4440.20410414827890555No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG4390.20180567814062958No Hit
CTGCCTAATCATCTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGT4380.20134598411297439No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT4180.19215210355987056No Hit
GAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCTTCCT4150.19077302147690497No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGGTAATC4110.18893424536628423No Hit
GGTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACACAGTCTTTGA3740.17192556634304207No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT3640.16732862606649015No Hit
GTTTGGGACTCTACCGTCCCCTTCTGCGTCTGCCGTTCCGGCCGACCACG3630.16686893203883493No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCGACAGGTCTTGCCC3590.16503015592821418No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCACGAATAGAAGGAAA3560.1636510738452486No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT3450.1585944395410415No Hit
CAGAATAGCTTGCCTTAGTGCTGTATGGTGAGGTGAACAATGTTCCGGAG3360.15445719329214475No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA3310.15215872315386877No Hit
TGGAAACCACCGTGAACGCCCGACAGGTCTTGCCCAAGGTCTTACATAAG3310.15215872315386877No Hit
ATCCTAACAGTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT3300.15169902912621358No Hit
CTCTTGGACTCTCAGCAATGTCAACGACCGACCTTGAGGCATACTTCAAA3290.15123933509855841No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG3120.14342453662842014No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC3060.14066637246248898No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA3040.1397469844071786No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCACGAA3040.1397469844071786No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCA3030.1392872903795234No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG3020.1388275963518682No Hit
TTTACAGACTGGGAGGAGTTGGGGGAGGAGATTACCTT3000.1379082082965578No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTACTGTT2910.13377096204766106No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT2890.1328515739923507No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA2860.13147249190938512No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG2860.13147249190938512No Hit
CCTTACCACAGGACCATATTGTACACAAAATCAAACAATGTTTTCGGAAA2820.12963371579876434No Hit
CTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAGACC2820.12963371579876434No Hit
ACCTTGAGGCATACTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTTG2820.12963371579876434No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT2810.12917402177110915No Hit
ATTGAAAACAGTACCAGCAGCGCCTCCTACTGCCTCCACCAATCGGCAGT2790.12825463371579876No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC2780.12779493968814357No Hit
CTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATT2760.1268755516328332No Hit
GTATACATGCATACAAAGGCATTAATGCCGGGTAACCACATTGTGTAAAG2750.12641585760517798No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC2740.1259561635775228No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGTCGGGCGTTC2740.1259561635775228No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC2720.12503677552221243No Hit
CATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGC2710.12457708149455722No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC2700.12411738746690203No Hit
CTATTCTGTGTTGGGGTGAGGTGATGAATCTGGCCACCTGGGTGGGAAGT2700.12411738746690203No Hit
GTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG2660.12227861135628126No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA2650.12181891732862607No Hit
GTCACCCTCAGGCCATGCAGTGGAACTCCAGCACATTCCACCAAGCTCTG2630.12089952927331568No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTG2570.11814136510738453No Hit
TTCTATTCGTGATCTCCTCGACACCGCCTCTGCTCTGTATCGGGAGGCCT2570.11814136510738453No Hit
GTACAAAGGTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACACAG2560.11768167107972932No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATG2540.11676228302441895No Hit
CTGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTC2530.11630258899676377No Hit
GGGTAAAGGTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA2520.11584289496910855No Hit
ATGTAAGACCTTGGGCAAGACCTGTCGGGCGTTCACGGTGGTCTCCATGC2490.11446381288614299No Hit
ATATGGTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA2450.11262503677552221No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC2430.11170564872021183No Hit
GGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATG2400.11032656663724624No Hit
ACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCTTGAGG2400.11032656663724624No Hit
GCTACTGTGTGTTGGGGTGAGGTGATGAATCTGGCCACCTGGGTGGGAAG2400.11032656663724624No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG2370.10894748455428067No Hit
CCTCGACACCGCCTCTGCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAAC2360.10848779052662547No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC2350.10802809649897029No Hit
GACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACAC2350.10802809649897029No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG2340.10756840247131509No Hit
GCCTTGGGTGGCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGA2340.10756840247131509No Hit
AGCAAGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA2340.10756840247131509No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC2330.10710870844365991No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG2320.1066490144160047No Hit
CCTCTGCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCT2320.1066490144160047No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG2310.10618932038834951No Hit
GTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG2310.10618932038834951No Hit
GTATGGTGAGGTGAACAATGTTCCGGAGACTCTAAGGCCTCCCGATACAG2310.10618932038834951No Hit
GTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTC2280.10481023830538393No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG2280.10481023830538393No Hit
ATCTTAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG2270.10435054427772875No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA2230.10251176816710797No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC2220.10205207413945278No Hit
GACCAATTTATGCCTACAGCCTCCCAGTACAAAGGTAATCTCCTCCCCCA2210.1015923801117976No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG2190.10067299205648719No Hit
GTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTGTATTTAAAGACT2180.10021329802883201No Hit
TCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGACCCGTATAA2180.10021329802883201No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)