FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences495510
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC30590.6173437468466833No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA27970.5644689310003834No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA27760.5602308732417105No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG20350.41068797804282453No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT18820.37981070008677925No Hit
AGCGAGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA18800.37940707553833425No Hit
CTATCAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG17650.35619866400274464No Hit
TTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA17640.35599685172852213No Hit
GGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAAT15060.3039292849791124No Hit
CCCTCATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATG14160.2857661802990858No Hit
GTATACATGCATATAAAGGCATCAATGCAGGATAGCCACATTGTGTAAAA13930.28112449799196787No Hit
GACTTCGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG13510.2726483824746221No Hit
GACGCAAACTGCACTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAG13190.26619038969950154No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA13060.2635668301346088No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT13040.26316320558616374No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG12590.2540816532461504No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA12390.25004540776170003No Hit
GTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGAAACATAGAGGTTC11690.2359185485661238No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA11270.227442433048778No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG10930.2205808157252124No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG10340.20867389154608385No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT10150.204839458335856No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9960.20100502512562815No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG9940.2006014005771831No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC9840.1985832778349579No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9630.19434522007628505No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG9520.19212528505983734No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG9480.19131803596294727No Hit
CCTCTCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9330.1882908518496095No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT9250.18667635365582935No Hit
TTATTGATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA9210.18586910455893926No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG9180.18526366773627173No Hit
ATGCAGGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCC9040.1824382958971565No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA8830.17820023813848357No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8690.17537486629936833No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8390.16932049807269278No Hit
GGCAATACCAGGTGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGGT8170.1648806280397974No Hit
CATATAAAGGCATCAATGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA8030.16205525620068212No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7970.16084438255534703No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA7960.1606425702811245No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC7930.16003713345845694No Hit
ATATAAAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTTGA7910.1596335089100119No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT7640.15418457750600392No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC7600.15337732840911383No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG7570.15277189158644627No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT7440.15014833202155356No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA7380.14893745837621844No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA7280.14691933563399326No Hit
GACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG7260.14651571108554823No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA7250.14631389881132573No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG7240.14611208653710317No Hit
GCTTGTATACATGCATATAAAGGCATCAATGCAGGATAGCCACATTGTGT7170.14469940061754555No Hit
GACAGAAACTGCACTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAG7130.1438921515206555No Hit
GGGCGAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAAT7060.14247946560109787No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT6980.14086496740731771No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC6910.13945228148776007No Hit
ATCCTGCATTGATGCCTTTATATGCATGTATACAAGCTAAGCAGGCTTTC6850.13824140784242497No Hit
GTATTAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGACCTAT6820.13763597101975744No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6710.1354160360033097No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT6650.1342051623579746No Hit
CTCCTCGACACCGCCTCTGCTCTGTATCGGGAGGCCTTAGAGTCTCCGGA6590.13299428871263952No Hit
CTTTTACACAATGTGGCTATCCTGCATTGATGCCTTTATATGCATGTATA6550.13218703961574943No Hit
GTTCAGAGCCAACTCAAACAATCCAGATTGGGACTTCAACCCCAACAAGG6380.12875623095396663No Hit
GTTTGCGTCCGAAGGTTTTGTACAGCAACAAGAGGGAAACATAGAGGTTC6350.12815079413129907No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT6350.12815079413129907No Hit
GTTTTAATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA6310.127343545034409No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT6210.12532542229218382No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6180.12471998546951625No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCTCCTCC6060.12229823817884602No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG6030.12169280135617849No Hit
TCCTAGTACAAAGATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGT5990.12088555225928842No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT5970.12048192771084339No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC5970.12048192771084339No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG5930.11967467861395331No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5920.11947286633973077No Hit
AGCGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA5910.11927105406550825No Hit
CCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATG5870.11846380496861818No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5640.11382212266150028No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5610.11321668583883272No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5520.11140037537083006No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC5440.1097858771770499No Hit
GAAGATGAGGCATAGCAGCAGGATGAAGAGGAATATGATAAAACGCCGCA5370.10837319125749229No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG5300.10696050533793465No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC5300.10696050533793465No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAAC5260.10615325624104457No Hit
GCACGGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATG5260.10615325624104457No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGACCGGC5200.10494238259570947No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5140.10373150895037436No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA5140.10373150895037436No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC5080.10252063530503926No Hit
GTCCCATGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5040.10171338620814918No Hit
TAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT5000.1009061371112591No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)