FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences415735
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG37710.9070682045052738No Hit
CTCTTACAGTGAACGAAAGAAGGAGATTAAAATTGATTATGCCTGCTAGG29340.7057380302356069No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT26360.6340577531360121No Hit
GTTCTATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC17050.4101170216604328No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16620.39977389442794087No Hit
CAGTTAACGAAAGAAGGAGATTAAAATTGATTATGCCTGCTAGGTTCTAT16560.3983306673722443No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG16380.39400098620515467No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCA13700.3295368443840427No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT13240.3184721036237026No Hit
CTCTTACAGTTAACGAAAGAAGGAGATTAAAATTGATTATGCCTGCTAGG12210.29369670583424534No Hit
GTGTAAAGGGAGCGGCAAAGCCTAGAAGCCCCACAATTCGTTGACAGACT12190.2932156301490132No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12170.292734554463781No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT12040.2896075625097719No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT11860.2852778813426822No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTGAGA10910.2624267862941537No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGGTT10730.2580971051270641No Hit
ACAATATGTAGGCCCTCTTACAGTGAACGAAAGAAGGAGATTAAAATTGA10050.24174053182917005No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9880.23765138850469653No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT9640.23187848028191038No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9480.2280298748000529No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGAACAATATGTT9210.2215353530494185No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT9170.22057320167895414No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA9030.2172056718823289No Hit
ATGTAGGCCCTCTTACAGTGAACGAAAGAAGGAGATTAAAATTGATTATG8850.21287599071523927No Hit
CCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGACCCGTAT8760.21071115013169445No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTCTTTCG8560.20590039327937265No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC8300.19964640937135433No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG7930.19074650919455904No Hit
TTATTAATAGATGTCAACAATATGTAGGCCCTCTTACAGTGAACGAAAGA7890.1897843578240947No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG7700.18521413881438897No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTA7650.18401144960130852No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7480.179922306276835No Hit
GTTTGGAAGTAATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCC7410.17823854137852238No Hit
GCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAAC7310.17583316295236148No Hit
GGATATTATTAATAGATGTCAACAATATGTAGGCCCTCTTACAGTGAACG6920.166452187090334No Hit
GATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC6670.16043874102493175No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG6640.15971712749708347No Hit
CCTCTAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGACCCGTAT6580.15827390044138692No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6530.1570712112283065No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA6480.15586852201522605No Hit
CTCCCAGTACAAAGATCATTAATCTAGTCTCCTCCCCCAACTCCTCCCAG6370.15322260574644905No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6110.14696862183843074No Hit
ATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGGAT6110.14696862183843074No Hit
GCCTAGAAGCCCCACAATTCGTTGACAGACTTTCCAATCAATAGGTCTAT6100.14672808399581466No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTTGGGGGAGGAGACTA6030.145044319097502No Hit
ATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGA6020.14480378125488594No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5990.14408216772703766No Hit
CATGTATACAAGCTAAGCAGGCTTTCACCTTCTCGCCAACTTACAAGGCC5750.13830925950425152No Hit
GTTTCTCTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATG5720.13758764597640324No Hit
CAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTGTGGTG5660.1361444189207067No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC5610.13494172970762625No Hit
TATTAATAGATGTCAACAATATGTAGGCCCTCTTACAGTGAACGAAAGAA5600.13470119186501017No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT5550.1334985026519297No Hit
ATTGACAACAGTGCCAACAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT5420.13037151069792055No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCACGAATAGAAGGAAA5400.12989043501268838No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5390.12964989717007228No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTAATAATATCCTCCTTT5380.1294093593274562No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC5380.1294093593274562No Hit
ACCTTGAGGCATACTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTTG5380.1294093593274562No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5380.1294093593274562No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGAACAAT5370.1291688214848401No Hit
ATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAG5340.12844720795699183No Hit
TTATTATCCTGAACACATAGTTAATCATTACTTCCAAACTAGGCATTATT5310.12772559442914355No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT5240.12604182953083093No Hit
ATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGG5120.12315537541943786No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA5110.12291483757682177No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5050.12147161052112523No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAAGAAGGAGATTAAAATTGA4970.11954730778019652No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA4910.11810408072449997No Hit
GGACATCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCG4860.11690139151141954No Hit
AGTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGTAGGGTTTAAATGTATA4820.11593924014095519No Hit
ATTATTAATAGATGTCAACAATATGTAGGCCCTCTTACAGTGAACGAAAG4820.11593924014095519No Hit
ATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG4700.1130527860295621No Hit
AAGCTGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGA4680.11257171034432993No Hit
GTTAATCATTACTTCCAAACTAGGCATTATTTACATACTCTGTGGAAGGC4650.11185009681648164No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA4630.11136902113124947No Hit
GACAACTATTGTGGTTTCACATTTCCTGTCTCACTTTTGGAAGAGAAACT4620.11112848328863338No Hit
CTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTG4610.1108879454460173No Hit
CAATAGGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGA4590.11040686976078512No Hit
TCACCATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA4580.11016633191816903No Hit
ATATGTAGGCCCTCTTACAGTGAACGAAAGAAGGAGATTAAAATTGATTA4560.10968525623293686No Hit
ATCCTGAACACATAGTTAATCATTACTTCCAAACTAGGCATTATTTACAT4470.10752041564939205No Hit
ATGTAAGACCTTGGGCAACACCTGTCGGGCGTTCACGGTGGTTTCCATGC4450.10703933996415987No Hit
CATTTAAAGGAGGATATTATTAATAGATGTCAACAATATGTAGGCCCTCT4390.10559611290846332No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT4360.10487449938061505No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTCTTTCGTTCAC4310.10367181016753461No Hit
ATAATATCCTCCTTTAAATGAATATGAGGAAAAGAGGGAGTTTGCCAGTC4220.1015069695839898No Hit
CCCTCTTACAGTGAACGAAAGAAGGAGATTAAAATTGATTATGCCTGCTA4210.10126643174137372No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGT4200.10102589389875763No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC4200.10102589389875763No Hit
GATTAAAATTGATTATGCCTGCTAGGTTCTATCCTAACCGTACCAAATAT4190.10078535605614154No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)