FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences415735
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG45261.0886742756804215No Hit
CTCTTACAGTGAACGAAAGAAGGAGATTAAAATTGATTATGCCTGCTAGG25910.6232335502182881No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT24330.585228571084946No Hit
GTTCTATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC16810.4043441134376466No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16500.3968874403165478No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG14990.3605662260815183No Hit
CAGTTAACGAAAGAAGGAGATTAAAATTGATTATGCCTGCTAGGTTCTAT14620.35166632590472297No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCA13980.33627190397729323No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT12350.29706423563087064No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGGTT12140.2920129409359327No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11750.28263196507390526No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT11400.2742131405823421No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT11310.2720482999987973No Hit
GTGTAAAGGGAGCGGCAAAGCCTAGAAGCCCCACAATTCGTTGACAGACT10850.2609835592384572No Hit
CTCTTACAGTTAACGAAAGAAGGAGATTAAAATTGATTATGCCTGCTAGG10850.2609835592384572No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTGAGA10820.2602619457106089No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGAACAATATGTT10270.24703236436672402No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT10260.24679182652410792No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT9830.23644869929161605No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA9510.2287514883279012No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTCTTTCG9220.2217758908920346No Hit
GTTTGGAAGTAATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCC9070.21816782325279324No Hit
CCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGACCCGTAT8990.21624352051186455No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8720.2097489987612301No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7800.18761951724054987No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7740.18617629018485332No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG7740.18617629018485332No Hit
ACAATATGTAGGCCCTCTTACAGTGAACGAAAGAAGGAGATTAAAATTGA7670.1844925252865407No Hit
GATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC7650.18401144960130852No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTA7600.18280876038822808No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA7530.18112499548991545No Hit
ATGTAGGCCCTCTTACAGTGAACGAAAGAAGGAGATTAAAATTGATTATG7510.18064391980468328No Hit
TTATTAATAGATGTCAACAATATGTAGGCCCTCTTACAGTGAACGAAAGA7490.18016284411945108No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG7330.17631423863759366No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC7110.1710224061000397No Hit
CCTCTAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGACCCGTAT6760.16260358160847657No Hit
GGATATTATTAATAGATGTCAACAATATGTAGGCCCTCTTACAGTGAACG6740.16212250592324437No Hit
ATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGGAT6670.16043874102493175No Hit
GCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAAC6640.15971712749708347No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGAACAAT6610.15899551396923522No Hit
GTTTCTCTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATG6590.15851443828400302No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTTGGGGGAGGAGACTA6500.15634959770045823No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6220.14961453810720773No Hit
ATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGA6210.1493740002645916No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA6040.1452848569401181No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTAATAATATCCTCCTTT5920.142398402828725No Hit
CTCCCAGTACAAAGATCATTAATCTAGTCTCCTCCCCCAACTCCTCCCAG5890.14167678930087677No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT5850.14071463793041242No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5800.13951194871733194No Hit
GGACATCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCG5780.13903087303209977No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5780.13903087303209977No Hit
TTATTATCCTGAACACATAGTTAATCATTACTTCCAAACTAGGCATTATT5680.13662549460593887No Hit
ATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGG5670.1363849567633228No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG5470.131574199911001No Hit
ACCTTGAGGCATACTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTTG5170.1243580646325183No Hit
TATTAATAGATGTCAACAATATGTAGGCCCTCTTACAGTGAACGAAAGAA5160.12411752678990222No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5070.12195268620635742No Hit
CAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTGTGGTG5040.12123107267850913No Hit
GCCTAGAAGCCCCACAATTCGTTGACAGACTTTCCAATCAATAGGTCTAT5040.12123107267850913No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC4950.11906623209496435No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4910.11810408072449997No Hit
GACAACTATTGTGGTTTCACATTTCCTGTCTCACTTTTGGAAGAGAAACT4840.11642031582618736No Hit
CATGTATACAAGCTAAGCAGGCTTTCACCTTCTCGCCAACTTACAAGGCC4820.11593924014095519No Hit
ATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAG4730.11377439955741037No Hit
CATTTAAAGGAGGATATTATTAATAGATGTCAACAATATGTAGGCCCTCT4700.1130527860295621No Hit
CAATAGGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGA4670.11233117250171382No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT4630.11136902113124947No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA4600.11064740760340121No Hit
CTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTG4600.11064740760340121No Hit
ATTATTAATAGATGTCAACAATATGTAGGCCCTCTTACAGTGAACGAAAG4590.11040686976078512No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC4540.10920418054770466No Hit
ATTGACAACAGTGCCAACAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT4510.1084825670198564No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTCTTTCGTTCAC4480.10776095349200814No Hit
ATATTTGTCTGATTTTTAGGCCCATATTAACATTGACATAGCTTACTACT4420.10631772643631159No Hit
AGTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGTAGGGTTTAAATGTATA4370.10511503722323114No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA4280.10295019663968633No Hit
ATCCTGAACACATAGTTAATCATTACTTCCAAACTAGGCATTATTTACAT4270.10270965879707024No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC4260.10246912095445415No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCACGAATAGAAGGAAA4240.10198804526922198No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT4230.10174750742660589No Hit
GATTAAAATTGATTATGCCTGCTAGGTTCTATCCTAACCGTACCAAATAT4210.10126643174137372No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)