FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences568997
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC25890.4550111863507189No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT23490.4128317021003625No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC22700.3989476218679536No Hit
CCCTTTACACAATGTGGTTACCCAGCCTTAATGCCTTTGTATGCATGTAT19990.3513199542352596No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT19070.3351511519392896No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG18540.32583651583400264No Hit
CATTTAAACCCTGCTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT17120.30088032098587514No Hit
GTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG17100.3005288252837888No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG16710.2936746590931059No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA16560.29103844132745865No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG16280.28611750149825044No Hit
GTGCTGGAGTTCCACTGCATGGCCTGAGGATGACTGTCTCGTAGAGGTGG15070.26485201152202914No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG14730.25887658458656193No Hit
GGATATTATTAATCGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG13940.244992504354153No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC13210.23216291122800295No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCGATTAATAATATCCTCTTTC12890.22653897999462214No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG12390.21775158744246456No Hit
CATGTATACAAGCGAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC11690.20544923786944394No Hit
GTTCTATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC11620.2042190029121419No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGGTT11440.20105554159336514No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATG11240.19754058457250212No Hit
TCCACAGAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTATG11080.19472861895581173No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTGAGA10870.19103791408390552No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGA10710.18822594846721513No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG10480.18418374789322264No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCAGCTCACCATACAG10400.18277776508487742No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA10390.18260201723383426No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC10070.17697808600045342No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG9980.17539635534106507No Hit
AGTCTGTACAACATCGTGAAGCCCTTTTTACCGCTGTCACTAATTTTCTT9630.16924518055455479No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC9630.16924518055455479No Hit
ATTGACAACAGTACCAGCAGCGCCTCCTACTGCCTCCACCAATCGGCAGT9440.1659059713847349No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC9410.16537872783160543No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTAGTGACAGCGGTAAAAA9410.16537872783160543No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTACTTCCGAGAGAGGACAACAGAGT9230.1622152665128287No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATCATT9090.15975479659822459No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT9080.15957904874718143No Hit
GTATACATGCATACAAAGGCATTAAGGCTGGGTAACCACATTGTGTAAAG9000.15817306593883623No Hit
GAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTATGTGTTCA8950.15729432668362048No Hit
TTATTAATCGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAA8900.1564155874284047No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8610.15131889974815332No Hit
TGCAAGGTCCAACTGGTGATCGGGAAAGAATCCCAGAGGATTGGGAACAG8580.15079165619502388No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG8460.14868268198250606No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG8440.14833118628041977No Hit
GTGCTGGGATGAACATGTGAGTGCATGTGTCTTTTTGGGAGAACAGTTTA8440.14833118628041977No Hit
ATTCAGAGCCAATTCAAACAATCCAGATTGGGACTTCAACCCCAACAAGG8430.1481554384293766No Hit
GGCCTACATATTGTTGACATCGATTAATAATATCCTCTTTCAAATGAATG8410.1478039427272903No Hit
ATGTAAGACCTTGGGCAAGACCTGTCGGGCGTTCACGGTGGTCTCCATGC8280.14551922066372933No Hit
GACCCTGCATTCGGAGCCAATTCAAACAATCCAGATTGGGACTTCAACCC8260.14516772496164304No Hit
CACACCAGGTAGGAGTGGGATCCTTCGGGCCAGGGTTCACTCCACCACAC8150.14323449860016838No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC8110.14253150719599578No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCGTGAAGCCCTTTTTACC8100.1423557593449526No Hit
GCTTTGGGACATGGACATTGACCCATATAAAGAATTTGGAGCTTCTGTGG7790.1369075759626149No Hit
ATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCCAGTC7770.13655608026052862No Hit
TTTTTTGGGTCACCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTC7760.13638033240948547No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG7720.13567734100531284No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT7680.13497434960114024No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA7600.13356836679279505No Hit
GTACAACATCGTGAAGCCCTTTTTACCGCTGTCACTAATTTTCTTTTGTC7590.1333926189417519No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA7540.13251387968653613No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCACGAATAGAAGGAAA7530.13233813183549298No Hit
GCCTTAGAGTCTCCGGAACATTGTTCAGCTCACCATACAGCACTAAGGCA7490.13163514043132038No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGAGTGGCTTTGGGACATGGACATTGA7480.13145939258027722No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC7460.13110789687819094No Hit
GCACGGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATG7430.13058065332506147No Hit
ATATAAAACACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTTGGA7260.1275929398573279No Hit
CCATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATC7200.12653845275106898No Hit
ACATATTGTTGACATCGATTAATAATATCCTCTTTCAAATGAATGTGAGG7190.12636270490002582No Hit
ATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG7150.12565971349585323No Hit
ATGTTGTTGAGTTCCACTGCATGGCCTGAGGATGACTGTCTCGTAGAGGT7090.12460522638959431No Hit
TCCTCAGGCCATGCAGTGGAACTCCAGCACATTCCACCAAGCACTGCTGG7080.12442947853855119No Hit
CTTATATAAAACACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTT7060.12407798283646486No Hit
GTACAAAGATCATTAATCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTA7050.12390223498542172No Hit
CTCTTATATAAAACACCAGCCTTCCACAGAGTATGTAAATAATGTCTAGT6980.12267200002811966No Hit
GATCCATGGCCAGAGGCATGGCAGGTAGGAGTGGGATCCTTCGGGCCAGG6940.12196900862394705No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT6930.12179326077290391No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC6740.11845405160308402No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA6740.11845405160308402No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTTGGGGGAGGAGACTA6530.11476334673117784No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCACGAA6520.1145875988801347No Hit
GTATAGGCCCCTCACTCTGGGATCCAGCAGTGCTTGGTGGAATGTGCTGG6500.1142361031780484No Hit
ATCCTGAACACATAGTTAATCATTACTTCCAAACTAGACATTATTTACAT6470.11370885962491895No Hit
GCTTACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC6290.11054539830614221No Hit
CCTCCACCTTATAGACCAACAAATGCCCCTATCTTATCAACACTTCCGGA6240.10966665905092646No Hit
GGCCTATACTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGT6220.10931516334884016No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA6180.10861217194466755No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6140.10790918054049495No Hit
GATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC6120.10755768483840865No Hit
CTATAAGGTGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTC6100.10720618913632232No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6070.1066789455831929No Hit
CCTTACCACAGGAACATATTGTACACAAAATCAAACAATGTTTTCGGAAA6050.10632744988110657No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAA6030.10597595417902027No Hit
GCCTGAGGATGACTGTCTCGTAGAGGTGGAGAAATGGGAGTAGGCTGTCT5940.10439422351963192No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC5930.10421847566858876No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5910.10386697996650246No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC5870.10316398856232986No Hit
GCCTTCCACAGAGTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAAC5830.10246099715815725No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCT5740.1008792664987689No Hit
CCATTGATCCTTGTTGGGGTTGAAGTCCCAATCTGGATTGTTTGAATTGG5700.10017627509459627No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)