FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489342

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489342
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441319
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCTAACCTTACCAAATATTTGCCCTTAGAGAAAGGCATCAAACCTTAT13910.3151915054643013No Hit
TCTATGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTATTCG13020.29502468735767096No Hit
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT12430.2816556731072082No Hit
CCTTTGCAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT11700.2651143503905338No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG11450.25944951384372755No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG10810.24494753228390345No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA10230.23180511149531294No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT9350.21186488685055482No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9100.20620005030374855No Hit
CTTCCAGGCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAG9020.20438730260877053No Hit
GTATACATGCGTACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG8650.19600334451949725No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTACGCATGTAT8600.19487037721013598No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG8560.19396400336264696No Hit
ATGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAGAAGATATTATCA8330.18875235373958518No Hit
GTATGTAAATAATGTCTAGCTTGGAAGTAATGATTAACTATATGTTCAGG8200.18580663873524594No Hit
GGATAATAAGGTTTGATGCCTTTCTCTAAGGGCAAATATTTGGTAAGGTT8090.18331411065465117No Hit
TCGCCAAAGAATCCCAGAGGATTGGGAACAGAAAGATTCGTCCCCATGCC7370.16699938139984907No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTATTCG7270.16473344678112656No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTCTCTAAGGGCAAATATTTGGTA7070.16020157754368156No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG7060.1599749840818093No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA6830.15476333445874751No Hit
GTATCCATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAC6780.15363036714938627No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6730.152497399840025No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCTTCTTTC6600.14955168483568576No Hit
CTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTA6600.14955168483568576No Hit
GTCTAGCTTGGAAGTAATGATTAACTATATGTTCAGGATAATAAGGTTTG6510.14751234367883548No Hit
GGTCCTAGGAATCCTGATGTGGTGCTCTCCATATTCGGTGCAGGGTCCCC6380.1445666286744962No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATATGTT6310.14298047444139048No Hit
CTATATGTTCAGGATAATAAGGTTTGATGCCTTTCTCTAAGGGCAAATAT6150.13935497905143446No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6050.13708904443271194No Hit
GGCCATGCAGTGGAACTCCAGCACATTCCACCAAGCTCTGCTAGATCCCA5930.1343699228902449No Hit
GCCTAGCAGCCATGGAAACGAGGTGTATTTCCGAGAGAGGACAACAGAGT5840.13233058173339468No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCGTTAAC5530.12530618441535488No Hit
ACTATATGTTCAGGATAATAAGGTTTGATGCCTTTCTCTAAGGGCAAATA5400.12236046941101561No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGAGATTGAGACCT5300.12009453479229311No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5220.11828178709731509No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG5080.11510947863110359No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC5020.11374991785987007No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGAGAAAGGCATCAAAC4790.1085382682368083No Hit
ATCTTAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG4730.1071787074655748No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4620.10468617938498002No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC4600.10423299246123552No Hit
TCCACAGAGTATGTAAATAATGTCTAGCTTGGAAGTAATGATTAACTATA4510.10219365130438526No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC4470.10128727745689625No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4450.10083409053315176No Hit
GTTTTCCATTCAGGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGT4420.100154310147535No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)