FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441319
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT14780.3349051366471872No Hit
TCTATGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTATTCG11900.26964621962797886No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGAGAAAGGCATCAAACCTTAT11090.2512921492163265No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG10560.23928269573709718No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG10380.2352040134233967No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG9970.22591368148663438No Hit
GTATACATGCGTACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG9510.21549038224051084No Hit
CCTTTGCAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT9500.21526378877863858No Hit
GTATGTAAATAATGTCTAGCTTGGAAGTAATGATTAACTATATGTTCAGG9300.21073191954119355No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTACGCATGTAT9000.20393411568502604No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8920.202121367990048No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG8720.19758949875260298No Hit
CTTCCAGGCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAG8630.19555015759575275No Hit
ATGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAGAAGATATTATCA8570.19419059682451922No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT8500.19260444259141346No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7660.17357059179414436No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTCTCTAAGGGCAAATATTTGGTA7570.1715312506372941No Hit
GGATAATAAGGTTTGATGCCTTTCTCTAAGGGCAAATATTTGGTAAGGTT7520.17039828332793286No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCTTCTTTC7500.16994509640418837No Hit
GTCTAGCTTGGAAGTAATGATTAACTATATGTTCAGGATAATAAGGTTTG7340.16631960101423235No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA7170.16246751216240407No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG7120.1613345448530428No Hit
GTATCCATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAC6380.1445666286744962No Hit
GGTCCTAGGAATCCTGATGTGGTGCTCTCCATATTCGGTGCAGGGTCCCC6290.14252728751764598No Hit
CTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTA5860.13278376865713917No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTATTCG5850.1325571751952669No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATATGTT5770.1307444275002889No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5740.13006464711467217No Hit
CTATATGTTCAGGATAATAAGGTTTGATGCCTTTCTCTAAGGGCAAATAT5540.12553277787722714No Hit
TCGCCAAAGAATCCCAGAGGATTGGGAACAGAAAGATTCGTCCCCATGCC5440.12326684325850462No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCGTTAAC5360.12145409556352661No Hit
GGCCATGCAGTGGAACTCCAGCACATTCCACCAAGCTCTGCTAGATCCCA5230.11850838055918733No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG5010.11352332439799782No Hit
GCCTAGCAGCCATGGAAACGAGGTGTATTTCCGAGAGAGGACAACAGAGT4940.11193717016489207No Hit
ATCTTAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG4850.1098978290080418No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGAGATTGAGACCT4840.10967123554616956No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGAGAAAGGCATCAAAC4830.10944464208429731No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAAT4790.1085382682368083No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG4770.10808508131306378No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG4740.10740530092744704No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC4580.10377980553749103No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4570.10355321207561877No Hit
ACTATATGTTCAGGATAATAAGGTTTGATGCCTTTCTCTAAGGGCAAATA4550.10310002515187427No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC4480.10151387091876853No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG4460.101060683995024No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)