FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences204552
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGTTT7530.36812155344362313No Hit
ATATGATCCTGTGGCAATGTACCCCAACTTCCAATTACATATCCCATGAA6950.3397669052368102No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6170.30163479213109623No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5040.24639211545230555No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCCCTGAATTTCAT4990.24394774922758028No Hit
GCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGTAAATGAAAAAAG4700.2297704251241738No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA4670.22830380538933864No Hit
CCTATTAGCAATGTCTTCATGTAAATGAATTTTGGGAAAAGAGGGAGTTT4460.2180374672454926No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA4370.2136376080409871No Hit
GTTCATATACTGTTTACTTAGAAAGGCCTTGTATGTTGGCGAGAAAGTAA3990.19506042473307522No Hit
TTCTTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACTCCCTCTTTTC3780.18479408658922913No Hit
GGATAATAAGGTTTAATCCCTTTGTCTAGAGGCAAATATTTAGTGCTGTT3740.18283859360944893No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCCGTCCG3720.18186084711955883No Hit
GGATCATATTGTACAAAAAATCAAACACTGTTTTAGAAAACTTCCTATCA3650.17843873440494348No Hit
CAATATGATCCTGTGGCAATGTACCCCAACTTCCAATTACATATCCCATG3600.17599436818021824No Hit
CACTCAACCCTGTTCCAAATATTGCCTCTCACATCTCGTCAATCTCCTCG3380.16523915679142712No Hit
GTACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA3260.1593726778520865No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACAATTCCGGA3250.15888380460714147No Hit
TTATTATCCTGATCAGGTAGTTAATCATTACTTTCAAACCCGACATTATT3230.15790605811725136No Hit
GTATACATGCATACAAAGGCATTAAAGCAGGGTAACCACATTGGGTAAAT3220.1574171848723063No Hit
CACTAAACCCTGTTCCAAATATTGCCTCTCACATCTCGTCAATCTCCTCG3190.15595056513747116No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACTGGG3180.1554616918925261No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC3130.15301732566780085No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT3080.1505729594430756No Hit
GCCTAATCATCTCTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGC3070.15008408619813057No Hit
GTACAAAACCTACGGATGGAAATTGCACCTGTATTCCCATCCCATCATCT2960.144706480503735No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG2920.1427509875239548No Hit
CATGAAGACATTGCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGT2900.1417732410340647No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCT2760.13492901560483397No Hit
TCCAAAGAGTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACC2690.13150690289021863No Hit
GAATAACCCCATCTCTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA2630.12857366342054832No Hit
GCACTAAATATTTGCCTCTAGACAAAGGGATTAAACCTTATTATCCTGAT2600.12710704368571316No Hit
CTGACATACTTTCCAATCAATAGGGCGATTGATAGGAAGTTTTCTAAAAC2580.12612929719582308No Hit
GTCTAGGAGATCCCGGACGGAAGGAAAGAAGTCAGAAGGCAAAAATGAGA2560.12515155070593295No Hit
TTCTAAAACAGTGTTTGATTTTTTGTACAATATGATCCTGTGGCAATGTA2520.12319605772615277No Hit
CCATTTACCCAATGTGGTTACCCTGCTTTAATGCCTTTGTATGCATGTAT2510.12270718448120772No Hit
GTTTTATACAGCAACATGAGGGAAACATAGAGTTGCCTTGAGCAGGAGTC2450.11977394501153742No Hit
CTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGT2400.11732957878681216No Hit
GTATATGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAG2380.11635183229692204No Hit
TCCCGATACAGAGCTGAGGCTGTGTCTAGGAGATCCCGGACGGAAGGAAA2370.11586295905197701No Hit
GCTTTAATGCCTTTGTATGCATGTATACAAGCGAAACAGGCTTTTACTTT2360.11537408580703196No Hit
GACAAAGGCTTGCCTGAGTGCTGTATGGTGAGGTGAGCAATGCTCAGGAG2310.1129297195823067No Hit
CATGTATACAAGCGAAACAGGCTTTTACTTTCTCGCCAACATACAAGGCC2300.11244084633736165No Hit
GATCAGGATAATAAGGTTTAATCCCTTTGTCTAGAGGCAAATATTTAGTG2220.10852986037780124No Hit
GTACGGGACCCTGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG2200.10755211388791114No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAGTACCACATC2190.1070632406429661No Hit
CAATAGGGCGATTGATAGGAAGTTTTCTAAAACAGTGTTTGATTTTTTGT2190.1070632406429661No Hit
GGGTAAATGGAGCGGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT2180.10657436739802105No Hit
GGAAAATACAGGCCCCTGACTCTGGGATCTTGCAGAGCTTGGTGAAAAGC2170.106085494153076No Hit
ATTTAGTGCTGTTAGGATAAAATCTAGCAGGCATAATTAATTTTAGTCTC2170.106085494153076No Hit
CTTTAATCCTGAATGGCAAACTCCCTCTTTTCCCAAAATTCATTTACATG2160.10559662090813093No Hit
CTATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATA2160.10559662090813093No Hit
GGGTAAAGGTTCATATACTGTTTACTTAGAAAGGCCTTGTATGTTGGCGA2150.10510774766318588No Hit
GTGGTCTCCATGCAACGTGCAGAGGTGAAGCGAAGTGCACACGGACCGGC2140.10461887441824083No Hit
GGTCTCCATGCAACGTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAG2130.1041300011732958No Hit
TCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTG2120.10364112792835074No Hit
CTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGCCTTGGATGGCTT2110.10315225468340568No Hit
GATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACAC2100.10266338143846063No Hit
GAGAATGGCTTGCCTGAGTGCTGTATGGTGAGGTGAGCAATGCTCAGGAG2100.10266338143846063No Hit
GCCTAGTCATCTCTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGC2100.10266338143846063No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT2080.10168563494857054No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)