FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences204552
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGTTT7430.3632328209941726No Hit
ATATGATCCTGTGGCAATGTACCCCAACTTCCAATTACATATCCCATGAA6740.32950056709296416No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA5890.28794634127263485No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5090.2488364816770308No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACAATTCCGGA4900.23954789002307483No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC4740.23172591810395402No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCCCTGAATTTCAT4420.21608197426571238No Hit
GGATCATATTGTACAAAAAATCAAACACTGTTTTAGAAAACTTCCTATCA4320.21119324181626187No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA4160.20337126989714108No Hit
GGATAATAAGGTTTAATCCCTTTGTCTAGAGGCAAATATTTAGTGCTGTT4080.19946028393758064No Hit
CCTATTAGCAATGTCTTCATGTAAATGAATTTTGGGAAAAGAGGGAGTTT4020.19652704446791036No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT3930.19212718526340491No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCCGTCCG3860.18870507254878957No Hit
CTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGT3850.18821619930384448No Hit
GCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGTAAATGAAAAAAG3830.1872384528139544No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACTGGG3740.18283859360944893No Hit
GTTCATATACTGTTTACTTAGAAAGGCCTTGTATGTTGGCGAGAAAGTAA3670.17941648089483359No Hit
CACTAAACCCTGTTCCAAATATTGCCTCTCACATCTCGTCAATCTCCTCG3660.17892760764988855No Hit
TTCTTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACTCCCTCTTTTC3660.17892760764988855No Hit
GTATATGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAG3620.17697211467010832No Hit
CACTCAACCCTGTTCCAAATATTGCCTCTCACATCTCGTCAATCTCCTCG3330.16279479056670187No Hit
GTATACATGCATACAAAGGCATTAAAGCAGGGTAACCACATTGGGTAAAT3260.1593726778520865No Hit
GTACAAAACCTACGGATGGAAATTGCACCTGTATTCCCATCCCATCATCT3110.15203957917791075No Hit
GTCTAGGAGATCCCGGACGGAAGGAAAGAAGTCAGAAGGCAAAAATGAGA3050.14910633970824044No Hit
ATGTGGTACTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACC2880.1407954945441746No Hit
GCCTAATCATCTCTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGC2860.13981774805428449No Hit
GTATAAAACCTACGGATGGAAATTGCACCTGTATTCCCATCCCATCATCT2860.13981774805428449No Hit
CTCTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGCCTTGGATGGC2810.13737338182955924No Hit
TTATTATCCTGATCAGGTAGTTAATCATTACTTTCAAACCCGACATTATT2780.13590676209472408No Hit
CAATATGATCCTGTGGCAATGTACCCCAACTTCCAATTACATATCCCATG2750.13444014235988894No Hit
CTATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATA2710.13248464938010873No Hit
GTACGGGACCCTGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG2680.1310180296452736No Hit
CTGCCGTACCGTCCGACCACGGGGCGCACCTCTCTTTACGCGGTCTCCCC2670.13052915640032853No Hit
GTCTAGAGGCAAATATTTAGTGCTGTTAGGATAAAATCTAGCAGGCATAA2640.12906253666549336No Hit
CTCCTAGACACAGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGA2630.12857366342054832No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG2580.12612929719582308No Hit
GTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC2530.12368493097109781No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA2520.12319605772615277No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAGTACCACATC2500.12221831123626266No Hit
CTTTAATCCTGAATGGCAAACTCCCTCTTTTCCCAAAATTCATTTACATG2500.12221831123626266No Hit
GTACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA2500.12221831123626266No Hit
GCACTAAATATTTGCCTCTAGACAAAGGGATTAAACCTTATTATCCTGAT2480.12124056474637256No Hit
TCCAAAGAGTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACC2420.11830732527670226No Hit
GCTTTAATGCCTTTGTATGCATGTATACAAGCGAAACAGGCTTTTACTTT2390.1168407055418671No Hit
CCATTTACCCAATGTGGTTACCCTGCTTTAATGCCTTTGTATGCATGTAT2390.1168407055418671No Hit
GTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACCTGATCAGG2360.11537408580703196No Hit
GCCTAGTCATCTCTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGC2290.11195197309241661No Hit
CATGAAGACATTGCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGT2280.11146309984747153No Hit
CTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGCCTTGGATGGCTT2260.11048535335758145No Hit
GGGTAAAGGTTCATATACTGTTTACTTAGAAAGGCCTTGTATGTTGGCGA2250.1099964801126364No Hit
ATTTAGTGCTGTTAGGATAAAATCTAGCAGGCATAATTAATTTTAGTCTC2220.10852986037780124No Hit
GTTTTATACAGCAACATGAGGGAAACATAGAGTTGCCTTGAGCAGGAGTC2200.10755211388791114No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGCCACCCGTG2100.10266338143846063No Hit
GGGTAAATGGAGCGGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT2080.10168563494857054No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)