FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences491414
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG57871.177622127167724No Hit
ATCCTAAACTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT32000.6511820990040984No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG31170.6342920633111796No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG27580.5612375715791573No Hit
GTTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG23650.4812642700452165No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGTTT21310.4336465790555417No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT20940.42611728603580684No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG19250.3917267314321529No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCA17900.3642549866304175No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGA17820.3626270313829073No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT17510.35631870479880506No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA17340.3528592998978458No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATG17300.3520453222740907No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT15870.322945622224845No Hit
TATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAA15290.31114294668039577No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA15020.30564859772004865No Hit
CTCTTCTACTGTACCTGTCTTTAATCCTGACTGGAAGACTCCCTCTTTTC13630.27736287529455816No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT13570.2761419088589255No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT13390.2724790095520274No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGAAGACTCCCTCTTTTCCTCAC13240.26942659346294573No Hit
GTTTGGAAGTAATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCC13090.266374177373864No Hit
GATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC12930.2631182668788435No Hit
ATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAA12750.2594553675719455No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT12550.2553854794531698No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC12530.2549784906412923No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12460.2535540297997208No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG11380.2315766339583325No Hit
ATTGATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT11200.2279137346514344No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC11110.22608228499798538No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT11060.2250648129682915No Hit
CAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGA10910.2220123968792098No Hit
GTTCTATCCTAAACTTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC10810.21997745281982198No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG10470.21305864301790342No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC10130.2061398332159849No Hit
CTTCTACTGTACCTGTCTTTAATCCTGACTGGAAGACTCCCTCTTTTCCT10110.20573284440410733No Hit
ATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTA9960.20268042831502564No Hit
CTTTAATCCTGACTGGAAGACTCCCTCTTTTCCTCACATTCATTTGAAAG9770.1988140346021888No Hit
ATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGG9700.1973895737606173No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9580.19494764088935193No Hit
ATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGGAT9440.19209871920620902No Hit
ATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGA8880.1807030324736373No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8720.1774471219786168No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA8320.16930734574106557No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC8300.16890035692918803No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTACTCTTCTACTGTACCTGTC8250.16788288489949413No Hit
GCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAAC8230.16747589608761654No Hit
GTTTCTCTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATG8220.16727240168167778No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG8220.16727240168167778No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTACTCTTCTACTG8140.16564444643416754No Hit
ATCTAAGGGCAAATATTTGGTAAGTTTAGGATAGAACCTAGCAGGCATAA8050.1638129967807185No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA7930.16137106390945313No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC7920.16116756950351435No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTA7820.15913262544412654No Hit
GTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGG7540.15343478207784067No Hit
ACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATGTGAGG7410.15078935480063652No Hit
GGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATG7330.1491613995531263No Hit
CTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATG7140.14529500584028945No Hit
GCTTTACTCTTCTACTGTACCTGTCTTTAATCCTGACTGGAAGACTCCCT7030.14305656737496286No Hit
ATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAG6930.14102162331557505No Hit
CTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGGTT6930.14102162331557505No Hit
TTATTATCCTGAACACATAGTTAATCATTACTTCCAAACTAGGCATTATT6860.13959716247400358No Hit
GATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTA6780.13796920722649333No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTACTC6700.1363412519789831No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA6690.1361377575730443No Hit
GTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGTTTAGGATAGAAC6670.13573076876116674No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6670.13573076876116674No Hit
GCCTTTATCTAAGGGCAAATATTTGGTAAGTTTAGGATAGAACCTAGCAG6490.1320678694542687No Hit
GGCCTAAAATTCAGGCAACTATTGTGGTTTCACATTTCCTGTCTTACTTT6420.13064340861269724No Hit
TCGTTGTCTAACAACAGTAGTTTCCGGAAGTGTTGATAAGATAGGGGCAT6410.13043991420675846No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6400.13023641980081968No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG6300.12820147574143187No Hit
ACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATA6290.1279979813354931No Hit
CTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATAT6280.1277944869295543No Hit
CAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATT6240.12698050930579918No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACAG6180.1257595428701665No Hit
GATTAAAATTGATTATGCCTGCTAGGTTCTATCCTAAACTTACCAAATAT6140.12494556524641137No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG5980.12168965475139089No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5870.1194512162860643No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5840.11884073306824795No Hit
CATTTGAAAGAGGATATTATCAATAGATGTCAACAATATGTAGGCCCTCT5830.11863723866230919No Hit
ATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAG5800.11802675544449284No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5770.1174162722266765No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG5740.11680578900886014No Hit
CCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTA5720.1163988001969826No Hit
CTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTG5680.11558482257322746No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT5610.114160361731656No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT5600.1139568673257172No Hit
ATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGTTTAG5520.11232891207820697No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGT5480.11151493445445185No Hit
TCACCATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA5450.1109044512366355No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA5390.10968348480100282No Hit
GTCTTTAATCCTGACTGGAAGACTCCCTCTTTTCCTCACATTCATTTGAA5320.10825902395943136No Hit
GGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGTTTAGGATAGAA5310.10805552955349257No Hit
GTAGTAACCTATGTCAATGTTAATATGGGCATAAAAATCAGGCAACTATT5310.10805552955349257No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC5290.10764854074161502No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5270.10724155192973746No Hit
GCTAGGTTCTATCCTAAACTTACCAAATATTTGCCCTTAGATAAAGGCAT5240.10663106871192111No Hit
CCTTTATCTAAGGGCAAATATTTGGTAAGTTTAGGATAGAACCTAGCAGG5210.10602058549410477No Hit
TCCTCAGGCCATGCAGTGGAACTCCAGCACATTCCACCAAGCTCTGCTAG5170.10520660787034963No Hit
GGTTACTACTAATTCCCTGGATCCTGGGTCTTCCAAATTACTTCCCACCC5030.10235768618720673No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)