FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences595407
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAAAGGGAGCAGCAAAACCTAAAAGACCCACAATTCTTTGACATACT34410.5779240082834095No Hit
GGACCATATTGTCCACAAAATCAAACAATGTTTTCGGAAACTTCCTGTAA25660.4309657091703658No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACATTGCCACAG25250.4240796631547832No Hit
CATATAAAGGCATTAATGCTGGGTATCCACATTGTGTAAAGGGAGCAGCA23160.3889776237094962No Hit
GTATGTAGATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG22450.3770530074386092No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT20650.3468215859067831No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA20470.34379844375360047No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAACTCAGACAAATATT18960.31843764013523523No Hit
GTATACACGCATATAAAGGCATTAATGCTGGGTATCCACATTGTGTAAAG18520.31104773709412215No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC15590.26183770093398295No Hit
ATATGGTCCTGTGGCAATGTACCCCAACTTCCAATTACATATCCCATGAA14910.25041694168862644No Hit
CCCTTTACACAATGTGGATACCCAGCATTAATGCCTTTATATGCGTGTAT14700.2468899425099134No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCACTTACAGTTAATG14160.23782051605036555No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA14020.23546918326455682No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGTCTTGTAAGTTGGCGA13560.22774337553975685No Hit
TTCCCCCACTGTGTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC12960.21766623502914814No Hit
GGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTACTGTT12340.20725318983485247No Hit
AGTCTGTACAACACCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT12160.20423004768166986No Hit
CCTACCGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11920.20019919147742635No Hit
GGGTATCCACATTGTGTAAAGGGAGCAGCAAAACCTAAAAGACCCACAAT11840.19885557274267854No Hit
ACCATATTGTCCACAAAATCAAACAATGTTTTCGGAAACTTCCTGTAAAT11830.19868762040083504No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTA11780.19784785869161767No Hit
GTACAACACCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC11040.18541938539520025No Hit
CTATAATCCTGAATGGCAAACTCCCTTTTTTCCACATATTCATTTGAAAG10830.1818923862164872No Hit
GTCTATAATCCTGAATGGCAAACTCCCTTTTTTCCACATATTCATTTGAA10710.17987695811436547No Hit
CTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATAT10490.17618200659380895No Hit
GTATCCACATTGTGTAAAGGGAGCAGCAAAACCTAAAAGACCCACAATTC10330.17349476912431328No Hit
CATTAATGCTGGGTATCCACATTGTGTAAAGGGAGCAGCAAAACCTAAAA10320.1733268167824698No Hit
GGTTCAGATATTGTTTACACAGAAAGGTCTTGTAAGTTGGCGAGAAAGTG10310.1731588644406263No Hit
CACTTACAGTTAATGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG10230.1718152457058785No Hit
TATCAATAGATGTCAACAATATGTGGGCCCACTTACAGTTAATGAAAAAA10200.17131138868034806No Hit
CAATAGATGTCAACAATATGTGGGCCCACTTACAGTTAATGAAAAAAGGA9430.15837905835840022No Hit
ACCTAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT9400.15787520133286978No Hit
GCCTAGCAGCCATGGAAAAGAGGTGTATTTCCGAGAGAGGACAACAGAGT9340.1568674872818089No Hit
ATGTTAATATGGGCCTAAAACTCAGACAAATATTGTGGTTTCACATTTCC9310.15636363025627847No Hit
CTACTGTACCTGTCTATAATCCTGAATGGCAAACTCCCTTTTTTCCACAT9310.15636363025627847No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACACCTTG9160.1538443451286263No Hit
ATGTAATTGGAAGTTGGGGTACATTGCCACAGGACCATATTGTCCACAAA9110.1530045834194089No Hit
GTCTATAAGCGGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATAC9110.1530045834194089No Hit
GAGTATGTAGATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCA8400.14107996714852195No Hit
TTGTTTACACAGAAAGGTCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGTT8360.140408157781148No Hit
TGTTTACACAGAAAGGTCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGTTT8350.14024020543930454No Hit
CGCATATAAAGGCATTAATGCTGGGTATCCACATTGTGTAAAGGGAGCAG8310.1395683960719306No Hit
ATTTACAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGGACAATATGGT8140.1367132062605915No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAGCACCCAAG8120.13637730157690453No Hit
ACTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATA7990.13419392113293932No Hit
ATCCTAACAGTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT7960.13369006410740888No Hit
CCTGTAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT7940.13335415942372192No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC7900.13268235005634801No Hit
CCCTAAACTTCATGGGATATGTAATTGGAAGTTGGGGTACATTGCCACAG7900.13268235005634801No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACACCTTGAGTCCCTTTTTACC7890.13251439771450454No Hit
ATGTAAGACCTTAGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC7810.1311707789797567No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGAAGTTTCCGAAAAC7740.12999511258685237No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACACCTTGAG7620.12797968448473063No Hit
ATTATCAATAGATGTCAACAATATGTGGGCCCACTTACAGTTAATGAAAA7620.12797968448473063No Hit
GTCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGTTTAGCTTGTATACACGC7510.12613220872445235No Hit
TTCTTCTACTGTACCTGTCTATAATCCTGAATGGCAAACTCCCTTTTTTC7510.12613220872445235No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAGATAATGCCTAGTTT7410.12445268530601755No Hit
GCTTACTACTGCTTCCCTGGATGTAGGGTCTTCCAAATTACCTCCCACCC7390.1241167806223306No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATG7310.12277316188758279No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGAGCATGGACATTGA7250.1217654478365219No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAGATAATGCCTAGT7250.1217654478365219No Hit
CAATATGTGGGCCCACTTACAGTTAATGAAAAAAGGAGATTAAAATTGAT7210.121093638469148No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTAG7200.12092568612730452No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCATTAAC7170.12042182910177408No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG7170.12042182910177408No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA7120.11958206739255668No Hit
CCCTACATCCAGGGAAGCAGTAGTAAGCTATGTCAATGTTAATATGGGCC7050.11840640099965234No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG7020.1179025439741219No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC6950.11672687758121755No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6850.11504735416278276No Hit
GGTCTATTTACAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGGACAAT6820.11454349713725233No Hit
CAATAGGTCTATTTACAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGG6810.11437554479540885No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA6760.11353578308619147No Hit
GTCTAACAACAGTAGTTTCCGGAAGTGTTGATAAGATAGGGGCATTTGGT6670.11202421200960015No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6640.11152035498406972No Hit
ATTTGGTACTGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTC6610.1110164979585393No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT6610.1110164979585393No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAGTCTCCTCC6610.1110164979585393No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC6590.11068059327485234No Hit
TCACCATACAGCACTAAGGCAAGCTATTCTGTGCTGGGGTGAGTTGATGA6570.11034468859116536No Hit
GTTCAGATATTGTTTACACAGAAAGGTCTTGTAAGTTGGCGAGAAAGTGA6520.10950492688194798No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCGATTCG6440.10816130814720017No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTTGGGGGAGGAGACTA6390.10732154643798275No Hit
TCCACATTGTGTAAAGGGAGCAGCAAAACCTAAAAGACCCACAATTCTTT6350.10664973707060885No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCATATCACCATACAG6320.10614588004507841No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG6300.10580997536139146No Hit
GTTAATATGGGCCTAAAACTCAGACAAATATTGTGGTTTCACATTTCCTG6250.10497021365217406No Hit
CCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATG6200.10413045194295667No Hit
GGATATGTAATTGGAAGTTGGGGTACATTGCCACAGGACCATATTGTCCA6120.10278683320820883No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTAGGCAAGACCTGGCGGGCGTTC6110.10261888086636535No Hit
CGTGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGACC6100.10245092852452188No Hit
GCCTTAGAGTCTCCGGAACATTGTTCATATCACCATACAGCACTAAGGCA5980.10043550042240013No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)