FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences595407
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAAAGGGAGCAGCAAAACCTAAAAGACCCACAATTCTTTGACATACT33060.5552504421345399No Hit
GTATGTAGATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG26190.4398671832880702No Hit
GGACCATATTGTCCACAAAATCAAACAATGTTTTCGGAAACTTCCTGTAA24860.4175295218228875No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACATTGCCACAG23580.3960316220669223No Hit
CATATAAAGGCATTAATGCTGGGTATCCACATTGTGTAAAGGGAGCAGCA21930.3683194856627483No Hit
GTATACACGCATATAAAGGCATTAATGCTGGGTATCCACATTGTGTAAAG21300.3577384881266092No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAACTCAGACAAATATT19230.3229723533650091No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT19160.3217966869721048No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA17820.2992910731650787No Hit
ATATGGTCCTGTGGCAATGTACCCCAACTTCCAATTACATATCCCATGAA15740.26435698606163516No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC15200.2552875596020873No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA14840.24924127529572207No Hit
CCCTTTACACAATGTGGATACCCAGCATTAATGCCTTTATATGCGTGTAT14460.2428590863056699No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCACTTACAGTTAATG13890.23328580282059164No Hit
GGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTACTGTT12960.21766623502914814No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGTCTTGTAAGTTGGCGA12010.20171076255401768No Hit
AGTCTGTACAACACCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT11890.19969533445189594No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTA11430.1919695267270959No Hit
CTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATAT11320.19012205096681764No Hit
TTCCCCCACTGTGTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC11190.18793867052285243No Hit
GTCTATAATCCTGAATGGCAAACTCCCTTTTTTCCACATATTCATTTGAA11100.18642709944626112No Hit
GTACAACACCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC11070.18592324242073072No Hit
CCTACCGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11060.18575529007888722No Hit
GGGTATCCACATTGTGTAAAGGGAGCAGCAAAACCTAAAAGACCCACAAT10870.1825641955838611No Hit
ACCATATTGTCCACAAAATCAAACAATGTTTTCGGAAACTTCCTGTAAAT10390.17450248317537415No Hit
GGTTCAGATATTGTTTACACAGAAAGGTCTTGTAAGTTGGCGAGAAAGTG10270.1724870550732524No Hit
GTATCCACATTGTGTAAAGGGAGCAGCAAAACCTAAAAGACCCACAATTC9950.1671125801342611No Hit
TATCAATAGATGTCAACAATATGTGGGCCCACTTACAGTTAATGAAAAAA9690.16274581924633066No Hit
ATGTTAATATGGGCCTAAAACTCAGACAAATATTGTGGTTTCACATTTCC9440.15854701070024368No Hit
CAATAGATGTCAACAATATGTGGGCCCACTTACAGTTAATGAAAAAAGGA9350.1570354396236524No Hit
GTCTATAAGCGGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATAC9330.15669953493996544No Hit
CATTAATGCTGGGTATCCACATTGTGTAAAGGGAGCAGCAAAACCTAAAA9320.15653158259812194No Hit
CACTTACAGTTAATGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG9250.1553559162052176No Hit
CTATAATCCTGAATGGCAAACTCCCTTTTTTCCACATATTCATTTGAAAG9210.1546841068378437No Hit
GAGTATGTAGATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCA9010.15132506000097412No Hit
ATGTAATTGGAAGTTGGGGTACATTGCCACAGGACCATATTGTCCACAAA8460.14208768119958282No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAGCACCCAAG8440.14175177651589585No Hit
ATTTACAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGGACAATATGGT8280.13906453904640018No Hit
TTGTTTACACAGAAAGGTCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGTT8250.13856068202086977No Hit
ACTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATA8250.13856068202086977No Hit
TGTTTACACAGAAAGGTCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGTTT8200.13772092031165237No Hit
ACCTAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT8200.13772092031165237No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA8170.13721706328612193No Hit
CCTGTAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT8000.1343618734747828No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAGATAATGCCTAGTTT7880.13234644537266108No Hit
CTACTGTACCTGTCTATAATCCTGAATGGCAAACTCCCTTTTTTCCACAT7680.12898739853579147No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGAGCATGGACATTGA7660.12865149385210453No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGAAGTTTCCGAAAAC7590.1274758274592002No Hit
ATTATCAATAGATGTCAACAATATGTGGGCCCACTTACAGTTAATGAAAA7570.12713992277551323No Hit
CCCTAAACTTCATGGGATATGTAATTGGAAGTTGGGGTACATTGCCACAG7460.12529244701523495No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTAG7450.12512449467339148No Hit
ATCCTAACAGTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT7440.12495654233154799No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACACCTTG7410.12445268530601755No Hit
GCTTACTACTGCTTCCCTGGATGTAGGGTCTTCCAAATTACCTCCCACCC7370.12378087593864365No Hit
GTCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGTTTAGCTTGTATACACGC7340.12327701891311323No Hit
GTTAATATGGGCCTAAAACTCAGACAAATATTGTGGTTTCACATTTCCTG7290.12243725720389581No Hit
GGTCTATTTACAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGGACAAT7290.12243725720389581No Hit
GGATATGTAATTGGAAGTTGGGGTACATTGCCACAGGACCATATTGTCCA7220.12126159081099147No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC7180.12058978144361757No Hit
ATTTGGTACTGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTC7170.12042182910177408No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATG7120.11958206739255668No Hit
GCCTAGCAGCCATGGAAAAGAGGTGTATTTCCGAGAGAGGACAACAGAGT7070.1187423056833393No Hit
TTCTTCTACTGTACCTGTCTATAATCCTGAATGGCAAACTCCCTTTTTTC6930.1163909728975306No Hit
TCCTTTGTCTACGTCCCCTCAGCGCTGAATCCCGCGGACGACCCGTCTCG6910.11605506821384365No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAGTCTCCTCC6890.1157191635301567No Hit
CGCATATAAAGGCATTAATGCTGGGTATCCACATTGTGTAAAGGGAGCAG6890.1157191635301567No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACACCTTGAG6860.11521530650462623No Hit
CAATAGGTCTATTTACAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGG6840.11487940182093928No Hit
CCCTACATCCAGGGAAGCAGTAGTAAGCTATGTCAATGTTAATATGGGCC6780.11387168776987842No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCATATCACCATACAG6770.11370373542803494No Hit
GTTCAGATATTGTTTACACAGAAAGGTCTTGTAAGTTGGCGAGAAAGTGA6680.11219216435144364No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6600.11084854561669581No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6570.11034468859116536No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAGATAATGCCTAGT6570.11034468859116536No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACACCTTGAGTCCCTTTTTACC6510.10933697454010449No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA6490.10900106985641754No Hit
GTCTAACAACAGTAGTTTCCGGAAGTGTTGATAAGATAGGGGCATTTGGT6430.10799335580535666No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA6390.10732154643798275No Hit
CAATATGTGGGCCCACTTACAGTTAATGAAAAAAGGAGATTAAAATTGAT6390.10732154643798275No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6360.10681768941245232No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTTGGGGGAGGAGACTA6290.10564202301954799No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG6260.10513816599401753No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCATTAAC6120.10278683320820883No Hit
GTCTACGTCCCCTCAGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG6100.10245092852452188No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6070.10194707149899145No Hit
ATGTCAATGTTAATATGGGCCTAAAACTCAGACAAATATTGTGGTTTCAC6060.10177911915714796No Hit
CTTTATATGCGTGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACT6010.10093935744793059No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)