FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503511
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG20360.40436058000718955No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA16860.33484869248139565No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT16810.33385566551674145No Hit
CATTTAAACCCTGCTAAAACTAAACGTTGGGGCTACTCCCTTAATTTCAT16010.31796723408227423No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCGCTTACAGTTAACG15150.30088717029022205No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA14980.29751087861039777No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT12590.25004418969992714No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA12180.24190136858976266No Hit
GTGTAAAGGGAGCGGCAAAGCCTAGAAGCCCCACAATTCGTTGACATACT10900.21647987829461524No Hit
GGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTACGGTT10210.20277610618238728No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA10200.20257750078945644No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10140.2013858684318714No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG9770.19403746889343035No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9640.19145559878532942No Hit
GTTTAAATGTATACCCAGAGACAAAAGAAAATTGGTAACAGCGGTAAAAA9300.18470301542568088No Hit
GTTCTATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATCAAAC8790.17457414038620803No Hit
ACAATATGTAGGCCCGCTTACAGTTAACGAAAAAAGGAGATTAAAATTGA8700.1727866918498305No Hit
ATTTATAGGAAGTTTCCGAAAAACTTGTTTGATTTTGTGTACAATATGTT8560.17000621634879873No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG8310.16504108152552774No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCGTTAAC8270.16424665995380439No Hit
CCCTTAATTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG8240.16365084377501188No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTGAAAATCAGACAACTATT8130.16146618445277264No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT8070.1602745520951876No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACC8010.15908291973760255No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC7990.1586857089517409No Hit
ATGTAGGCCCGCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATG7670.152330336377954No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG7580.15054288784157643No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7400.14696799076882133No Hit
CCCACGTGTCCTGGCCAAAATTTGCAGTCCCCAACCTCCAATCACTCACC7160.1422014613384812No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7030.13961959123038026No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC6840.1358460887646943No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAATTAAGGGAG6790.1348530618000401No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT6680.1326684024778009No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG6630.13167537551314668No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCA6580.13068234854849248No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC6520.12949071619090746No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT6440.12790187304746073No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA6410.1273060568686682No Hit
GGTCTATTTATAGGAAGTTTCCGAAAAACTTGTTTGATTTTGTGTACAAT6370.12651163529694484No Hit
GCCTAGAAGCCCCACAATTCGTTGACATACTTTCCAATCAATAGGTCTAT6290.12492279215349815No Hit
CTCACTGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCT6260.12432697597470563No Hit
GATTAACTATGTGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGC6210.12333394901005142No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC6150.12214231665246637No Hit
GGGTATACATTTAAACCCTGCTAAAACTAAACGTTGGGGCTACTCCCTTA6080.1207520789019505No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT6060.12035486811608881No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6050.12015626272315798No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTA6050.12015626272315798No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTCTTACATAAGAGGACTCTTG6040.11995765733022715No Hit
GTCTGTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6030.11975905193729632No Hit
GGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATG5990.11896463036557293No Hit
ATGTTAATATGGGCCTGAAAATCAGACAACTATTGTGGTTTCACATTTCC5800.11519112789988699No Hit
GTACAATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCC5800.11519112789988699No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5740.11399949554230196No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT5600.1112190200412702No Hit
ACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATGTGAGG5600.1112190200412702No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA5600.1112190200412702No Hit
CAATAGATGTCAACAATATGTAGGCCCGCTTACAGTTAACGAAAAAAGGA5510.10943157150489266No Hit
TATCAATAGATGTCAACAATATGTAGGCCCGCTTACAGTTAACGAAAAAA5470.10863714993316928No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5410.10744551757558424No Hit
AATTAAGGGAGTAGCCCCAACGTTTAGTTTTAGCAGGGTTTAAATGTATA5350.10625388521799922No Hit
GACCTGACCGTTGCCGAGCAACGGGGTAAAGGTTCAGATATTGTTTACAC5320.1056580690392067No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC5280.10486364746748332No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA5260.10446643668162166No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCT5190.10307619893110578No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA5100.10128875039472822No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAACAGGCTTTCACTTT5100.10128875039472822No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5080.10089153960886653No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG5070.1006929342159357No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)