FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences535160
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN75801.416398833993572No Hit
GTTTTAGTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACA32460.6065475745571418No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT26770.5002242320053816No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAATTTTCG26060.4869571716869721No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCGCG22950.4288437102922491No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT17350.3242021077808506No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT16710.3122430674938336No Hit
GTACTCAAAATCAAGCAATGTTTTCGAAAATTGCCTGTAAATAGACCTAT16190.3025263472606323No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC16120.30121832722923986No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG16120.30121832722923986No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA14710.27487106659690563No Hit
CTAGTAAACTGAGCCATGAGAAACGGACTGAGGCCCACTCCCATAGGAAT14580.2724418865386053No Hit
GTACTAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAAATAGACCTAT13860.2589879662157112No Hit
GGAATCGTGCAGGTCTTGCATGGCCCCGTGCTGGTGGTTGATGTTCCTGG13180.24628148591075566No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA12560.23469616563270798No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA12130.22666118543986846No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT11420.21339412512145903No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG11340.2118992450855819No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA11300.21115180506764333No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA11230.20984378503625087No Hit
GGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTA11170.20872262500934302No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA11100.20741460497795053No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA10450.1952687046864489No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA10310.19265266462366396No Hit
GTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCC10160.18984976455639435No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10160.18984976455639435No Hit
GTTTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGT9850.1840571044173705No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC9810.18330966439943194No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACGAGAAAAACCCC9620.17975932431422378No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAATAAA9580.1790118842962852No Hit
ATTTTGAGTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTAC9520.17789072426937738No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG9510.17770386426489274No Hit
GTCTTTGGGTATACATTTAAACCCTAATAAAACCAAACGTTGGGGCTACT9220.17228492413483817No Hit
GCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA9080.16966888407205322No Hit
TTATTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA9060.16929516406308395No Hit
GCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGCAGAGACCTGACC8980.16780028402720681No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG8620.16107332386575976No Hit
CATGTATACAAACTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8610.16088646386127511No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA8530.159391583825398No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACCACC8480.1584572838029748No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT8320.15546752373122055No Hit
GTACAAAACCTTCGGACGGAAACTGCACTTGTATTCCCATCCCATCATCC8300.1550938037222513No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAATTTTCGAAAAC8070.15079602361910457No Hit
CCCAACACCACATCATCCACATAACCGAAAGCCAAACAGTGGGGGAAAGC7960.14874056356977353No Hit
GAACAAATGGCACTAGTAAACTGAGCCATGAGAAACGGACTGAGGCCCAC7910.14780626354735033No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATAC7870.14705882352941177No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCACCCATATCGTCAATCTTCTCG7730.1444427834666268No Hit
GGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG7670.14332162343971896No Hit
ATATTGTACTAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAAATAGA7530.140705583376934No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG7360.1375289633006951No Hit
GTCAACGAATTGTGGGTCTTTTGGGCTTTGCTGCCCCTTTTACACAATGT7340.13715524329172582No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT7260.1356603632558487No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG7240.13528664324687945No Hit
TTTTGAGTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACA7150.1336049032065177No Hit
GGAACATATTGTACTAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAA7070.13211002317064055No Hit
ACTAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAAATAGACCTATTG7060.1319231631661559No Hit
GGGTATACATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTA7030.131362583152702No Hit
ATCATATTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT6950.12986770311682488No Hit
GGATGATGGGATGGGAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTAC6860.1281859630764631No Hit
GCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGC6860.1281859630764631No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG6840.12781224306749384No Hit
GGAGTCGTGCAGGTCTTGCATGGCCCCGTGCTGGTGGTTGATGTTCCTGG6770.12650422303610134No Hit
GGTCTTGCATGGCCCCGTGCTGGTGGTTGATGTTCCTGGAAGTAGAGGAC6710.1253830630091935No Hit
GTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTC6640.12407504297780103No Hit
GGTATACATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAA6570.12276702294640855No Hit
GTACTAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAAATAGACCTATTGATTGGAAAGTATGTCAAAGAAT6550.12239330293743926No Hit
CTTTATATGCATGTATACAAACTAAGCAGGCTTTCACTTTCTCGCCAACT6550.12239330293743926No Hit
ATTCGTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAATTTTCG6500.12145900291501607No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG6470.12089842290156215No Hit
CCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATG6420.11996412287913895No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6350.11865610284774648No Hit
GGATATGTAATTGGAAGTTGGGGTACTTTACCGCAGGAACATATTGTACT6330.11828238283877719No Hit
CAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTAGTTTGTA6320.11809552283429256No Hit
CCCATATCGTCAATCTTCTCGAGGACTGGGGACCCTGCACCGAACATGGA6260.1169743628073847No Hit
TTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGG6250.11678750280290007No Hit
CAATTACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTA6250.11678750280290007No Hit
GCCCCGTGCTGGTGGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6240.11660064279841542No Hit
GTACTCAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAAATAGACCTAT6190.11566634277599223No Hit
TAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT6170.11529262276702296No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGTAC6080.11361088272666119No Hit
CCAACACCACATCATCCACATAACCGAAAGCCAAACAGTGGGGGAAAGCC6080.11361088272666119No Hit
CAATAGGTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGT5960.1113685626728455No Hit
CTATGGGAGTGGGCCTCAGTCCGTTTCTCATGGCTCAGTTTACTAGTGCC5890.11006054264145301No Hit
GCCCAGGATGATGGGATGGGAATACAAGTGCAGTTTCCGTCCGAAGGTTT5770.10781822258763733No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC5760.1076313625831527No Hit
GCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTACCAAGGTATGTTGC5750.10744450257866806No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCGCAGGAACATATTGTACTAAAA5730.10707078256969878No Hit
GACCTGACCGTTGCCGGGCAACGGGGTAAAGGTTCAGATATTGTTTACAC5690.10632334255176024No Hit
GGTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGTACAAT5650.10557590253382167No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5650.10557590253382167No Hit
ACCCTGTTCCGACTACTGCCTCACCCATATCGTCAATCTTCTCGAGGACT5640.10538904252933702No Hit
GTTTTATTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT5610.1048284625158831No Hit
GCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA5550.10370730248897526No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAA5460.1020255624486135No Hit
CCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGACTCAAGATG5430.10146498243515957No Hit
GTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGTACAATA5410.10109126242619029No Hit
ATCCTGGGCTTTCGCAAGATTCCTATGGGAGTGGGCCTCAGTCCGTTTCT5390.10071754241722103No Hit
GTAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTT5390.10071754241722103No Hit
TACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAG5370.10034382240825175No Hit
CCTTTATATGCATGTATACAAACTAAGCAGGCTTTCACTTTCTCGCCAAC5370.10034382240825175No Hit
TAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATGTAA5360.1001569624037671No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)