FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences535160
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN75601.412661633903879No Hit
GTTTTAGTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACA33030.6171985948127663No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCGCG26630.49760819194259664No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAATTTTCG25280.47238209133717024No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT24310.45425667090216004No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT20480.3826892891845429No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG17010.31784886762837283No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT16430.30701098736826365No Hit
GTACTCAAAATCAAGCAATGTTTTCGAAAATTGCCTGTAAATAGACCTAT15940.2978548471485163No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC14990.28010314672247555No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA14760.2758053666193288No Hit
GTACTAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAAATAGACCTAT14330.26777038642648926No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT14040.2623514462964347No Hit
GGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTA12950.24198370580760892No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA12940.2417968458031243No Hit
CTAGTAAACTGAGCCATGAGAAACGGACTGAGGCCCACTCCCATAGGAAT12670.236751625682039No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA11980.22385828537259886No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG11890.2221765453322371No Hit
GTTTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGT10980.20517228492413483No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA10730.20050078481201883No Hit
GGAATCGTGCAGGTCTTGCATGGCCCCGTGCTGGTGGTTGATGTTCCTGG10590.1978847447492339No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA10540.19695044472681067No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA10510.19638986471335676No Hit
GTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCC10510.19638986471335676No Hit
ATTTTGAGTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTAC10400.1943344046640257No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA10360.19358696464608716No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10250.1915315045967561No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA10190.1904103445698483No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC10080.1883548845205172No Hit
GTCTTTGGGTATACATTTAAACCCTAATAAAACCAAACGTTGGGGCTACT9940.18573884445773226No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACGAGAAAAACCCC9760.18237536437700874No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG9240.17265864414380747No Hit
GTACAAAACCTTCGGACGGAAACTGCACTTGTATTCCCATCCCATCATCC9010.16836086404066072No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG8960.16742656401823752No Hit
GCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA8880.1659316839823604No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAATAAA8870.16574482397787577No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT8710.16275506390612154No Hit
GCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGCAGAGACCTGACC8390.15677554376261305No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA8360.15621496374915914No Hit
GGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG8320.15546752373122055No Hit
ATCATATTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT8150.15229090365498169No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCACCCATATCGTCAATCTTCTCG7990.14930114358322744No Hit
TTATTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA7920.14799312355183497No Hit
GTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTC7710.14406906345765752No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATAC7700.14388220345317287No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACCACC7670.14332162343971896No Hit
CATGTATACAAACTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7630.1425741834217804No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG7580.1416398833993572No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT7340.13715524329172582No Hit
GTCAACGAATTGTGGGTCTTTTGGGCTTTGCTGCCCCTTTTACACAATGT7330.1369683832872412No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAATTTTCGAAAAC7320.13678152328275656No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG7130.1332311831975484No Hit
GAACAAATGGCACTAGTAAACTGAGCCATGAGAAACGGACTGAGGCCCAC7090.13248374317960984No Hit
CCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATG7070.13211002317064055No Hit
GGGTATACATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTA7070.13211002317064055No Hit
CCCAACACCACATCATCCACATAACCGAAAGCCAAACAGTGGGGGAAAGC6930.1294939831078556No Hit
GGATGATGGGATGGGAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTAC6680.12482248299573959No Hit
TTTTGAGTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACA6590.12314074295537783No Hit
GGAACATATTGTACTAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAA6560.1225801629419239No Hit
ACTAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAAATAGACCTATTG6510.12164586291950072No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGTAC6430.1201509828836236No Hit
ATATTGTACTAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAAATAGA6400.11959040287016967No Hit
GCCTATGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGC6390.11940354286568503No Hit
GGATATGTAATTGGAAGTTGGGGTACTTTACCGCAGGAACATATTGTACT6300.11772180282532327No Hit
GGTCTTGCATGGCCCCGTGCTGGTGGTTGATGTTCCTGGAAGTAGAGGAC6260.1169743628073847No Hit
TAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT6250.11678750280290007No Hit
CTTTATATGCATGTATACAAACTAAGCAGGCTTTCACTTTCTCGCCAACT6170.11529262276702296No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG6140.11473204275356903No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGG6050.11305030271320726No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG6010.11230286269526871No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC6010.11230286269526871No Hit
GTACTAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAAATAGACCTATTGATTGGAAAGTATGTCAAAGAAT5980.11174228268181478No Hit
GGTATACATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAA5930.11080798265939158No Hit
GCCCAGGATGATGGGATGGGAATACAAGTGCAGTTTCCGTCCGAAGGTTT5930.11080798265939158No Hit
TTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGG5880.10987368263696837No Hit
CCCATATCGTCAATCTTCTCGAGGACTGGGGACCCTGCACCGAACATGGA5830.1089393826145452No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5810.1085656626055759No Hit
CTATGGGAGTGGGCCTCAGTCCGTTTCTCATGGCTCAGTTTACTAGTGCC5770.10781822258763733No Hit
GTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGTACAATA5750.10744450257866806No Hit
GTTTTATTAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT5730.10707078256969878No Hit
ATTCGTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAATTTTCG5710.10669706256072951No Hit
ACCCTGTTCCGACTACTGCCTCACCCATATCGTCAATCTTCTCGAGGACT5670.10594962254279094No Hit
CAATTACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTTTA5590.10445474250691382No Hit
GGTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGTACAAT5570.10408102249794453No Hit
GTAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTT5560.1038941624934599No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5540.10352044248449063No Hit
ATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATACAAACTAAGC5530.10333358248000599No Hit
GCCCCGTGCTGGTGGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5530.10333358248000599No Hit
ATCCTGGGCTTTCGCAAGATTCCTATGGGAGTGGGCCTCAGTCCGTTTCT5530.10333358248000599No Hit
GCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTACCAAGGTATGTTGC5480.10239928245758279No Hit
CCTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA5440.10165184243964422No Hit
CCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGACTCAAGATG5400.10090440242170565No Hit
CTTCTGGACTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC5380.10053068241273638No Hit
CAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTAGTTTGTA5380.10053068241273638No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)