FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473086
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25530.5396481823600783No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG21940.4637634594978503No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATGGCTGAGAGTCCAA14320.3026933792164638No Hit
ATCCTAACCTTACTAAATATTTGCCCTTAGACAAAGGCATTAAACCGTAT13430.2838807320444908No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT13280.28071006117281005No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA12970.2741573413713363No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACGGTTAATGAAAAA12480.26379981652384554No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA12240.2587267431291562No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG11380.2405482301315194No Hit
CTCTTACGGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG10840.2291338149934684No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10100.2134918386931763No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT9830.20778463112415077No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT9740.2058822286011423No Hit
CTTCTAGGGGACCTGCCTCGTCGTCTAACAACAGTAGTTTCCGGAAGTGT9710.2052480944268061No Hit
CATGTATACAATCCAAGCAGGCTTTCACTTTCTCGCCAACTTATAAGGCC9520.20123191132267707No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCGCCGTG9180.19404505734686717No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9170.1938336792887551No Hit
GTTCTATCCTAACCTTACTAAATATTTGCCCTTAGACAAAGGCATTAAAC8840.18685820337105727No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG8690.18368753249937642No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8620.18220788609259203No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA8610.18199650803447998No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8330.17607792240734244No Hit
ATATGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTG8230.17396414182622189No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG8030.16973658066398076No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT7890.166777287850412No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGGCCGGC7850.16593177561796377No Hit
GGATAATACGGTTTAATGCCTTTGTCTAAGGGCAAATATTTAGTAAGGTT7740.16360661697873113No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT7740.16360661697873113No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA7700.16276110474628291No Hit
ATTCTGTGTTGGGGTGAGTTAATGAATCTGGCCACCTGGGTGGGAAGTAA7640.16149283639761058No Hit
CCCTAGCAGCCATGGAAAGGCGGTGTATTTCCGAGAGAGGACAACAGAGT7480.15811078746781768No Hit
CTCCTCGACACCGCCTCTGCTCTGTATCGGGAGGCCTTAGAGTCTCCGGA7100.15007842125955956No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6900.14585086009731846No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6860.14500534786487024No Hit
CCGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT6670.1409891647607412No Hit
CTCCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG6460.136550225540388No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC6390.13507057913360362No Hit
GGATTAAAGACAGGTACCGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC6360.13443644495926746No Hit
CTTATAAGGCCTTTCTGTGTAAACAATATATGAACCTTTACCCCGTTGCC6320.13359093272681924No Hit
GCGGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAAC6250.13211128632003483No Hit
GTCTAAGGGCAAATATTTAGTAAGGTTAGGATAGAACCTAGCAGGCATAA6230.13168853020381072No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTCC6120.1293633715645781No Hit
CCCTCGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGTCTCAATCGCCGC5980.12640407875100934No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5980.12640407875100934No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA5920.12513581040233698No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC5890.12450167622800083No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5830.12323340787932849No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT5700.12048549312387176No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5680.12006273700764765No Hit
GGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA5670.1198513589495356No Hit
GGGTAAAGGTTCATATATTGTTTACACAGAAAGGCCTTATAAGTTGGCGA5660.11963998089142354No Hit
GTTCAGGATAATACGGTTTAATGCCTTTGTCTAAGGGCAAATATTTAGTA5650.11942860283331148No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA5610.11858309060086326No Hit
GTTTAATGCCTTTGTCTAAGGGCAAATATTTAGTAAGGTTAGGATAGAAC5600.11837171254275121No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA5580.1179489564265271No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC5580.1179489564265271No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC5560.11752620031030298No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA5510.1164693100197427No Hit
GTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT5460.11541241972918242No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT5450.11520104167107037No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG5410.11435552943862215No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACGG5410.11435552943862215No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC5380.11372139526428597No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5370.11351001720617392No Hit
CTCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAG5360.11329863914806186No Hit
GTATAAAGAATTTGGAGCATCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5360.11329863914806186No Hit
CTCCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACACAGTCTTT5350.1130872610899498No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATACGGTTTAATGCC5330.1126645049737257No Hit
GTCTTTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5310.1122417488575016No Hit
GTGTAAACAATATATGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC5310.1122417488575016No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT5280.11160761468316543No Hit
CCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTGCCACTCGGGATTAAAG5270.11139623662505338No Hit
GGTTCATATATTGTTTACACAGAAAGGCCTTATAAGTTGGCGAGAAAGTG5250.11097348050882927No Hit
ATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGG5220.1103393463344931No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5190.10970521216015694No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC5130.1084369438114846No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA5100.10780280963714843No Hit
CTCCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG5040.1065345412884761No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT5020.10611178517225199No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGCCCGTGTG4970.1050548948816917No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4960.10484351682357965No Hit
ATTTTGTGTTGGGGTGAGTTAATGAATCTGGCCACCTGGGTGGGAAGTAA4950.10463213876546759No Hit
CAATATATGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCA4940.10442076070735554No Hit
GCCATGGAAAGGCGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCGGTT4930.10420938264924348No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA4890.10336387041679526No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA4830.10209560206812293No Hit
CCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTC4810.10167284595189881No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4780.10103871177756264No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)