FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences541276
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN31130.5751224883423613No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA29340.542052483391098No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC26900.49697381742401286No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA16320.30150976581263533No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG14300.2641905423480812No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT13080.24165120936453863No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT11160.20617947221011093No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT10890.20119125917276953No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA10640.19657254339745345No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACACCCAATTACATATCCCATGAA10520.1943555598253017No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC10400.19213857625314995No Hit
GTATCAGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG10280.18992159268099823No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG10250.1893673467879603No Hit
CTCGTTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA10030.18530287690568215No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGT9900.18290114470251775No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA9810.18123840702340396No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT9510.17569594809302463No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA9410.1738484617828982No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA9160.1692297460075821No Hit
GTTTCGCTTGTATACATGCATATAAAGGCATTAAAGCAGGATATCCACAT9060.16738225969745563No Hit
GTTGTGAGTATGCCCTGCGCCTGAGGGCTCCACCCCAACAGTCCCCCATG9050.16719751106644298No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG8990.16608901928036715No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA8970.16571952201834184No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA8730.1612855548740384No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG8650.15980756582593725No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT8580.15851432540884872No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA8550.1579600795158108No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG8500.15703633636074757No Hit
GTCCGGTAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC8260.1526023692164441No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG8190.1513091287993556No Hit
GGATAATAAGGTTTGATTCCTTTATCTAAGGGCAAATATTTAGTAACATT8170.1509396315373303No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC8110.14983113975125445No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG7970.14724465891707744No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT7610.14059370820062222No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG7580.1400394623075843No Hit
GATGTATACTTGCGGGAGAGCACGACGGAATTGTCAGTCCCGATGAGTTT7560.139669965045559No Hit
GTTTACAGGAAGTTTCCTAAAACACAGTTTGATTTTTTGTACAATATGTT7480.13819197599745786No Hit
GGAACATATTGTACAAAAAATCAAACTGTGTTTTAGGAAACTTCCTGTAA7480.13819197599745786No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA7340.13560549516328083No Hit
CCTGTAAACAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT7270.13431225474619232No Hit
CTTCAAAGACTGTGTGTTTAACGAGTGGGAGGAGTTGGGGGAGGAGATTA7230.13357326022214175No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG7210.13320376296011646No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCTGGTCTGTGCCAAGTGTTTGCTG7120.13154102528100267No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC7100.13117152801897736No Hit
ATGTAATTGGGTGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA7090.13098677938796474No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT7080.1308020307569521No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC6930.12803080129176242No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT6930.12803080129176242No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT6870.12692230950568656No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC6860.12673756087467392No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6860.12673756087467392No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT6840.12636806361264863No Hit
GCCTAGCAGCCATGGGAATGATGTATACTTGCGGGAGAGCACGACGGAAT6820.12599856635062334No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC6800.12562906908859806No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA6730.12433582867150954No Hit
TCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCACT6690.12359683414745898No Hit
CTACCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAGACC6610.12211884509935782No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC6590.12174934783733252No Hit
CGTTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT6590.12174934783733252No Hit
GTATACATCATTCCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC6550.12101035331328194No Hit
GTCCCAATGAGTTTTGCTCCAGACCTGCTGCGAGCAAAACAAGCGGCTAG6540.12082560468226931No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG6490.11990186152720608No Hit
GTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGCAAGACCTTGGG6340.11713063206201643No Hit
AAGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT6340.11713063206201643No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6330.11694588343100376No Hit
AAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTCTAAGT6320.11676113479999113No Hit
CATCGGGACTGACAATTCCGTCGTGCTCTCCCGCAAGTATACATCATTCC6310.11657638616897849No Hit
GTGTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCCCGTC6240.11528314575188997No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA6220.11491364848986468No Hit
GGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGA6130.1132509108107509No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC6120.11306616217973825No Hit
GTAATTGGGTGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAAAT6100.11269666491771296No Hit
CACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAATCT6090.11251191628670032No Hit
GACCAGACCGTTGCCGAGCAACGGGGTAAAGGTTCAGATATTGTTTACTT6070.11214241902467502No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA6050.11177292176264973No Hit
CATCATTCCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGG6050.11177292176264973No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG5990.11066442997657386No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5950.1099254354525233No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA5920.10937118955948537No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC5920.10937118955948537No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5880.10863219503543478No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAGG5850.10807794914239686No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT5840.10789320051138422No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCTGGTCTGTGCCAAGTG5810.10733895461834628No Hit
TGGAAACCACCGTGAACGTCCACCGGAACCTGCCCAAGGTCTTGCATAAG5750.10623046283227042No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA5740.10604571420125776No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGTACAC5680.10493722241518191No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGC5670.10475247378416927No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA5670.10475247378416927No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG5570.10290498747404281No Hit
GGGTAAAGGTTCAGATATTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA5470.10105750116391637No Hit
GCACAACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACA5420.10013375800885316No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)