FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489359

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489359
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences541276
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN31070.5740139965562855No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA27150.501592533199329No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC24860.45928509669743345No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA16250.3002165253955468No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG14810.27361272252972607No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT11730.21671014417783163No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA11100.20507098042403504No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG11070.2045167345309971No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT10760.19878952696960514No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC10720.19805053244555457No Hit
GTATCAGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG10540.19472505708732699No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA10200.18844360363289708No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT9920.18327064196454304No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT9770.1804994124993534No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACACCCAATTACATATCCCATGAA9650.17828242892720164No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA9510.17569594809302463No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA9330.17237047273479705No Hit
GTTTCGCTTGTATACATGCATATAAAGGCATTAAAGCAGGATATCCACAT9180.16959924326960737No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA9160.1692297460075821No Hit
GGATAATAAGGTTTGATTCCTTTATCTAAGGGCAAATATTTAGTAACATT8650.15980756582593725No Hit
GTTGTGAGTATGCCCTGCGCCTGAGGGCTCCACCCCAACAGTCCCCCATG8590.15869907403986136No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG8450.15611259320568435No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG8280.1529718664784694No Hit
CTCGTTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA8270.15278711784745674No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT8080.14927689385821652No Hit
CTTCAAAGACTGTGTGTTTAACGAGTGGGAGGAGTTGGGGGAGGAGATTA7980.14742940754809009No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA7940.14669041302403948No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA7940.14669041302403948No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG7800.14410393218986248No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGT7770.14354968629682455No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC7700.14225644587973602No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG7690.14207169724872337No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA7630.14096320546264754No Hit
GTATACATCATTCCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC7610.14059370820062222No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7610.14059370820062222No Hit
GTCCGGTAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC7550.13948521641454636No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG7510.1387462218904958No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC7490.1383767246284705No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT7460.13782247873543257No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC7450.13763773010441993No Hit
GTTTACAGGAAGTTTCCTAAAACACAGTTTGATTTTTTGTACAATATGTT7340.13560549516328083No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC7170.1324647684360659No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT7170.1324647684360659No Hit
CATCATTCCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGG7150.1320952711740406No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC7040.13006303623290152No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT7000.12932404170885092No Hit
ATGTAATTGGGTGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA6840.12636806361264863No Hit
CCTGTAAACAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT6830.12618331498163599No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT6800.12562906908859806No Hit
CTACCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAGACC6720.1241510800404969No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG6690.12359683414745898No Hit
GGAACATATTGTACAAAAAATCAAACTGTGTTTTAGGAAACTTCCTGTAA6680.12341208551644631No Hit
GTGTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCCCGTC6660.12304258825442103No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6620.12230359373037046No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCTGGTCTGTGCCAAGTGTTTGCTG6610.12211884509935782No Hit
GACCAGACCGTTGCCGAGCAACGGGGTAAAGGTTCAGATATTGTTTACTT6520.12045610742024403No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6520.12045610742024403No Hit
CGTTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT6510.12027135878923138No Hit
GATGTATACTTGCGGGAGAGCACGACGGAATTGTCAGTCCCGATGAGTTT6490.11990186152720608No Hit
GCCTAGCAGCCATGGGAATGATGTATACTTGCGGGAGAGCACGACGGAAT6480.11971711289619344No Hit
GTCCCAATGAGTTTTGCTCCAGACCTGCTGCGAGCAAAACAAGCGGCTAG6460.11934761563416815No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA6220.11491364848986468No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT6190.11435940259682677No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC6170.11398990533480148No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC6160.11380515670378882No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC6110.1128814135487256No Hit
TGGAAACCACCGTGAACGTCCACCGGAACCTGCCCAAGGTCTTGCATAAG6110.1128814135487256No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCTGGTCTGTGCCAAGTG6060.11195767039366238No Hit
GGGTAAAGGTTCAGATATTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA6000.11084917860758653No Hit
AAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTCTAAGT5940.10974068682151066No Hit
GGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGA5930.10955593819049801No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA5920.10937118955948537No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG5920.10937118955948537No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5900.10900169229746007No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC5880.10863219503543478No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA5870.10844744640442214No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA5830.10770845188037158No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA5770.1065999600942957No Hit
TCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCACT5740.10604571420125776No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG5730.10586096557024512No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT5730.10586096557024512No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAGG5690.10512197104619456No Hit
AAGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT5680.10493722241518191No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA5680.10493722241518191No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA5650.10438297652214396No Hit
CATCGGGACTGACAATTCCGTCGTGCTCTCCCGCAAGTATACATCATTCC5640.10419822789113133No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGTC5620.10382873062910604No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG5590.10327448473606811No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG5530.10216599294999223No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTACCGGACCGTGTG5470.10105750116391637No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)