FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences505300
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA21780.43103107065109836No Hit
GACCTTTAACCTAACCTCCTCCCCCAACTCCTCCCACTCAGTAAACACAC20470.4051058776964179No Hit
TGCAAGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA16740.3312883435582822No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16640.3293093211953295No Hit
ATTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT15690.31050860874727887No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT15120.29922818127844847No Hit
ATTCAACATCTCCTCGACACCGCCTCTGCTCTGTATCGGGAGGCCTTAGA13500.26716801899861464No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12390.2452008707698397No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG12070.23886799920839105No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG11500.22758757173956065No Hit
GATCATTAACCTAACCTCCTCCCCCAACTCCTCCCACTCAGTAAACACAC11340.22442113595883634No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT11060.21887987334256878No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA11050.21868197110627352No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA10710.21195329507223432No Hit
GGTTAGTGAGTGACTGGAGATTTGGGACTGCGAATTTTGGCCAAGACACA10310.20403720562042352No Hit
CTCACCAACCTGTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTC10240.2026518899663566No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG10130.20047496536710865No Hit
CCTCTTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9980.1975064318226796No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA9870.1953295072234316No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG9850.1949337027508411No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA9620.19038195131604987No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG9380.1856322976449634No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC9270.18345537304571544No Hit
GGCCTTGCATGGTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGAC9250.18305956857312486No Hit
ATTCGAGATCTCCTCGACACCGCCTCTGCTCTGTATCGGGAGGCCTTAGA9230.18266376410053434No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT9110.1802889372649911No Hit
GTTTTGCTTGTATACATGCATATAAAGGCATTAAAGCAGGATATCCACAT8970.17751830595685733No Hit
ATTTACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT8970.17751830595685733No Hit
CATGTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT8720.17257075004947556No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA8540.1690085097961607No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT8400.16623787848802693No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAATGATTAACTA8260.16346724717989314No Hit
CTGTAAGGGGTCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC7910.1565406689095587No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA7620.15080150405699583No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC7610.15060360182070057No Hit
GCGGCATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAAAACCAC7380.14605185038590937No Hit
CCCTTTTCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA7340.1452602414407283No Hit
TCTCATGGCTCAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTT7200.1424896101325945No Hit
GCACAACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACA7190.14229170789629922No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG7100.1405105877696418No Hit
GTATCAGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG6940.13734415198891747No Hit
GGTTTTGCATGGTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGAC6830.1351672273896695No Hit
GTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTGAG6780.13417771620819316No Hit
GTTGTAAATAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG6760.1337819117356026No Hit
GGTTTACATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTATAA6620.13101128042746882No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGAAGCTGGATCTTC6510.12883435582822086No Hit
GCGTAAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCGTTGACATACT6460.1278448446467445No Hit
GATGCGATGTTCTCCATGTTCGGTACAGGGTCCCCAGTCTTCGATAAGGT6460.1278448446467445No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAAAACCACATC6330.125272115574906No Hit
GTGCTGGTGAACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC6310.12487631110231547No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA6300.12467840886602019No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT6290.12448050662972493No Hit
GCCTAGCAGCCATGGAAATGATGTGTACTTGCGGGAGAGCACGACAGAAT6280.12428260439342965No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTAAAA6250.12368889768454383No Hit
TTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACC6250.12368889768454383No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6200.1226993865030675No Hit
GTTGTGAATAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG6180.12230358203047693No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAACCTTATCGAAGACTGGGGA6150.12170987532159114No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG6040.11953295072234316No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA6020.11913714624975262No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA5990.1185434395408668No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT5880.11636651494161883No Hit
TCTTTTGTCTTTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGG5780.11438749257866614No Hit
CAGTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT5740.11359588363348506No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5740.11359588363348506No Hit
TCCACATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGA5720.11320007916089453No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT5710.11300217692459925No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT5620.11122105679794182No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5610.11102315456164656No Hit
GGATATCCACATTGCGTAAAAGGGGCGGCAAACCCCAAAAGACCCACAAT5590.110627350089056No Hit
CCTAATGATTAACTAGTACAAAGACCTTTAACCTAACCTCCTCCCCCAAC5550.10983574114387493No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5370.10627350089056006No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA5300.10488818523649317No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC5280.10449238076390263No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT5250.10389867405501682No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT5250.10389867405501682No Hit
TCTCTTGGCTCAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTT5240.10370077181872156No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5180.10251335840094992No Hit
AAGCCAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTCTAAGT5130.10152384721947359No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG5110.10112804274688303No Hit
CATTAAAGCAGGATATCCACATTGCGTAAAAGGGGCGGCAAACCCCAAAA5110.10112804274688303No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC5100.10093014051058777No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC5060.1001385315654067No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)