FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences505300
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA21410.4237086879081734No Hit
GACCTTTAACCTAACCTCCTCCCCCAACTCCTCCCACTCAGTAAACACAC16820.3328715614486444No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16650.3295072234316248No Hit
TGCAAGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA16380.32416386305165246No Hit
ATTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT14760.29210370077181874No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT14210.28121907777557886No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG12400.245398773006135No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11820.2339204433010093No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA11290.22343162477735998No Hit
ATTCAACATCTCCTCGACACCGCCTCTGCTCTGTATCGGGAGGCCTTAGA10870.21511973085295866No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG10530.20839105481891945No Hit
GGTTAGTGAGTGACTGGAGATTTGGGACTGCGAATTTTGGCCAAGACACA10340.20463091232930933No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA10330.20443301009301407No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT10240.2026518899663566No Hit
CCTCTTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10210.2020581832574708No Hit
CTCACCAACCTGTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTC10130.20047496536710865No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG10040.1986938452404512No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA9820.19433999604195526No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA9760.19315258262418367No Hit
GATCATTAACCTAACCTCCTCCCCCAACTCCTCCCACTCAGTAAACACAC9610.19018404907975459No Hit
GTTTTGCTTGTATACATGCATATAAAGGCATTAAAGCAGGATATCCACAT9540.18879873342568773No Hit
ATTCGAGATCTCCTCGACACCGCCTCTGCTCTGTATCGGGAGGCCTTAGA9330.18464278646348703No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG9140.1808826439738769No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA9020.17850781713833366No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT8800.17415396793983773No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT8630.17078962992281813No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG8490.16801899861468436No Hit
ATTTACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT8130.16089451810805463No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAATGATTAACTA8030.15891549574510191No Hit
CATGTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT8000.1583217890362161No Hit
CTGTAAGGGGTCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC7930.15693647338214922No Hit
GGCCTTGCATGGTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGAC7860.15555115772808234No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC7720.15278052641994855No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG7660.15159311300217693No Hit
GTATCAGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG7460.1476350682762715No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAACCTTATCGAAGACTGGGGA7290.14427073025925194No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC7040.13932317435187017No Hit
TCTCATGGCTCAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTT6980.13813576093409857No Hit
GGTTTACATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTATAA6950.13754205422521273No Hit
GTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTGAG6940.13734415198891747No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGAAGCTGGATCTTC6870.1359588363348506No Hit
GGTTTTGCATGGTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGAC6860.13576093409855533No Hit
GCACAACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACA6710.13279240055412625No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTAAAA6700.132594498317831No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA6610.13081337819117356No Hit
GCCTAGCAGCCATGGAAATGATGTGTACTTGCGGGAGAGCACGACAGAAT6540.12942806253710668No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT6530.1292301603008114No Hit
CCCTTTTCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA6470.12804274688303977No Hit
GCGTAAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCGTTGACATACT6240.12349099544824857No Hit
GCGGCATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAAAACCAC6230.1232930932119533No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGGTTA6110.12091826637641007No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA5900.11676231941420939No Hit
TTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACC5880.11636651494161883No Hit
GATGCGATGTTCTCCATGTTCGGTACAGGGTCCCCAGTCTTCGATAAGGT5860.11597071046902829No Hit
GTGCTGGTGAACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC5820.11517910152384722No Hit
GTTGTAAATAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG5810.11498119928755196No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA5790.1145853948149614No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAAAACCACATC5780.11438749257866614No Hit
GTTGTGAATAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG5740.11359588363348506No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA5680.11240847021571343No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT5670.11221056797941817No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT5640.11161686127053236No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT5630.1114189590342371No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA5620.11122105679794182No Hit
TCTCTTGGCTCAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTT5580.11042944785276074No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5520.10924203443498913No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG5480.10845042548980803No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC5470.10825252325351277No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA5420.10726301207203642No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA5380.10647140312685532No Hit
GGATATCCACATTGCGTAAAAGGGGCGGCAAACCCCAAAAGACCCACAAT5360.1060755986542648No Hit
TCTTTTGTCTTTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGG5350.10587769641796953No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5350.10587769641796953No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA5340.10567979418167425No Hit
TCCACATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGA5300.10488818523649317No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT5290.10469028300019789No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5280.10449238076390263No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT5270.10429447852760736No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5270.10429447852760736No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC5240.10370077181872156No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT5240.10370077181872156No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG5220.103304967346131No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG5200.10290916287354046No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5180.10251335840094992No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC5170.10231545616465466No Hit
GGTTGGTGAGTGACTGGAGATTTGGGACTGCGAATTTTGGCCAAGACACA5060.1001385315654067No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)