FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489364

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489364
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences304451
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG11690.3839698342261973No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCATCTTTTCCTCAT10290.3379854229416228No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT9110.2992271334303385No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA8840.29035871125402773No Hit
GTGCTGGTGAACGGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8600.2824756693195293No Hit
CCTACTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8550.28083336891650873No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCGCG7990.26243960440267894No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA7780.2555419427099927No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7370.24207507940522446No Hit
TCTACGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTCCCTTCTATTCG6860.22532361529441516No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6690.21973979392414544No Hit
CTATTAATAATGTCCTCCTGTAAATGAATATGAGGAAAAGATGGAGTTTG6600.2167836531987085No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6490.21317059231206337No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG6480.21284213223145926No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6370.2092290713448141No Hit
GTGTAAAAGGGGCAGCAAACCCCAAAAGACCCACAATTCTCTGACATACT6290.20660139069998126No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG6270.20594447053877304No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA6070.19937526892669102No Hit
GCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCGGTT5980.19641912820125407No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC5840.19182068707279662No Hit
CTCCTACTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG5820.1911637669115884No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT5780.189849926589172No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT5690.18689378586373503No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5300.17408384272017502No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT5270.1730984624783627No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT5210.17112770199473806No Hit
GGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTGAGGTT4880.16028851933480265No Hit
GGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTA4810.1579892987705739No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG4480.1471501161106385No Hit
ATCCTAACCTCACCAAATATTTGCCCTTGGACAAAGGCATTAAACCATAT4480.1471501161106385No Hit
GTCTATTTACAGGCAGTTTTCTAAAACATTGCTTGATTTTTAGTACAATA4390.14419397538520157No Hit
GACAAATACTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACT4380.14386551530459746No Hit
GTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAC4370.14353705522399335No Hit
GCTTAGATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACAT4350.14288013506278513No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT4330.14222321490157694No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4310.14156629474036872No Hit
TTCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC4280.14058091449855642No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4240.13926707417614No Hit
GATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGT4230.1389386140955359No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA4230.1389386140955359No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC4120.13532555320889075No Hit
CTTTAATCCTGAGTGGCAAACTCCATCTTTTCCTCATATTCATTTACAGG4120.13532555320889075No Hit
CTTTTGGAGTGTGGATTCGCACTCCTACTGCTTACAGACCACCAAATGCC4110.13499709312828667No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA4100.13466863304768256No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA4090.13434017296707845No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC4080.13401171288647434No Hit
GTTCAGGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTG4020.13204095240284972No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGACCG4010.13171249232224563No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4000.13138403224164152No Hit
GCTATGTTAATGTCAATATGGGCCTAAAACTCAGACAAATACTGTGGTTT3980.1307271120804333No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG3970.1303986519998292No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT3800.12481483062955943No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA3740.12284407014593482No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC3630.11923100925928967No Hit
GGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAAT3620.11890254917868556No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG3540.11627486853385274No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC3540.11627486853385274No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC3540.11627486853385274No Hit
CTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCTAAAAC3510.11528948829204043No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA3490.11463256813083221No Hit
ATATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT3490.11463256813083221No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC3480.11430410805022811No Hit
CTATTACCAATTTTCTTTTGTCTCTGGGTATACATTTGAACCCTAATAAA3470.11397564796962402No Hit
CAGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAACA3460.11364718788901991No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3450.1133187278084158No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCATCTTTTC3440.11299026772781169No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC3410.11200488748599939No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA3400.11167642740539528No Hit
CATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA3400.11167642740539528No Hit
GTATTTGTCTGAGTTTTAGGCCCATATTGACATTAACATAGCTGACTACT3400.11167642740539528No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG3380.11101950724418709No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA3380.11101950724418709No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT3370.11069104716358298No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA3370.11069104716358298No Hit
GACTTTGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG3360.11036258708297887No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG3340.10970566692177065No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCTCCTCC3330.10937720684116657No Hit
CTCTAAGGCCTCCCGATACAAAGCTGAGGCGGTGTCGAGGAGATCTCGAA3320.10904874676056246No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG3320.10904874676056246No Hit
ATACTGCAGAACTCCTAGCAGCCTGTTTTGCTCGCAGCCGGTCTGGAGCG3300.10839182659935424No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG3280.10773490643814605No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC3210.10543568587391731No Hit
GGTCTATTTACAGGCAGTTTTCTAAAACATTGCTTGATTTTTAGTACAAT3180.104450305632105No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT3180.104450305632105No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG3160.10379338547089678No Hit
ATGTTAATGTCAATATGGGCCTAAAACTCAGACAAATACTGTGGTTTCAC3160.10379338547089678No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG3150.1034649253902927No Hit
GACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG3130.10280800522908448No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG3120.10247954514848037No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG3110.10215108506787626No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC3100.10182262498727218No Hit
GAATTAGTAGTCAGCTATGTTAATGTCAATATGGGCCTAAAACTCAGACA3090.10149416490666807No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA3090.10149416490666807No Hit
GTTCAAATGTATACCCAGAGACAAAAGAAAATTGGTAATAGAGGTAAAAA3080.10116570482606396No Hit
TAACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTT3080.10116570482606396No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGG3070.10083724474545985No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA3050.10018032458425166No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)