FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences304451
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG13880.4559025918784961No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT10170.33404390197437356No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCATCTTTTCCTCAT9260.3041540346394001No Hit
GTGCTGGTGAACGGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8980.29495715238248515No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCGCG8520.2798479886746964No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA8250.2709795664983856No Hit
CCTACTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7740.2542281023875763No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA7210.23681971811555882No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7130.234192037470726No Hit
GTGTAAAAGGGGCAGCAAACCCCAAAAGACCCACAATTCTCTGACATACT6740.22138209432716596No Hit
CTATTAATAATGTCCTCCTGTAAATGAATATGAGGAAAAGATGGAGTTTG6580.21612673303750027No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG6330.2079152310223977No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6250.20528755037756488No Hit
TCTACGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTCCCTTCTATTCG6240.20495909029696074No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT6210.20397371005514844No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG6200.20364524997454436No Hit
CTCCTACTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG6030.1980614286042746No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5970.19609066812064999No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA5940.19510528787883766No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA5840.19182068707279662No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT5660.18590840562192273No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG5510.18098150441286118No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGACCG5500.18065304433225707No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT5370.17638306328440373No Hit
TTCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC5340.17539768304259143No Hit
GGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTGAGGTT5290.1737553826395709No Hit
GCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCGGTT5220.17145616207534217No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT5020.1648869604632601No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC5010.164558500382656No Hit
GGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTA5010.164558500382656No Hit
GTCTATTTACAGGCAGTTTTCTAAAACATTGCTTGATTTTTAGTACAATA4840.15897467901238624No Hit
GTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAC4810.1579892987705739No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4630.15207701731970005No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA4470.1468216560300344No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4390.14419397538520157No Hit
CTTTTGGAGTGTGGATTCGCACTCCTACTGCTTACAGACCACCAAATGCC4380.14386551530459746No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA4370.14353705522399335No Hit
GCTTAGATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACAT4310.14156629474036872No Hit
GACAAATACTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACT4200.1379532338537236No Hit
ATCCTAACCTCACCAAATATTTGCCCTTGGACAAAGGCATTAAACCATAT4200.1379532338537236No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG4180.13729631369251538No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA4140.13598247337009897No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT4120.13532555320889075No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC4070.13368325280587026No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA4050.13302633264466204No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA3960.1300701919192251No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC3930.1290848116774128No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCATCTTTTC3920.12875635159680868No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC3910.12842789151620457No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT3900.12809943143560046No Hit
ATGTTAATGTCAATATGGGCCTAAAACTCAGACAAATACTGTGGTTTCAC3880.12744251127439227No Hit
GCTATGTTAATGTCAATATGGGCCTAAAACTCAGACAAATACTGTGGTTT3860.12678559111318405No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG3840.12612867095197586No Hit
CTTTAATCCTGAGTGGCAAACTCCATCTTTTCCTCATATTCATTTACAGG3840.12612867095197586No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT3840.12612867095197586No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT3800.12481483062955943No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT3740.12284407014593482No Hit
GTATTTGTCTGAGTTTTAGGCCCATATTGACATTAACATAGCTGACTACT3700.12153022982351841No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA3690.1212017697429143No Hit
GATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGT3660.12021638950110197No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG3640.11955946933989378No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC3630.11923100925928967No Hit
GTTCAGGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTG3630.11923100925928967No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC3590.11791716893687326No Hit
CTCTAAGGCCTCCCGATACAAAGCTGAGGCGGTGTCGAGGAGATCTCGAA3540.11627486853385274No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG3540.11627486853385274No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATATGGTTTAATGCC3490.11463256813083221No Hit
CAGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAACA3490.11463256813083221No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG3490.11463256813083221No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA3470.11397564796962402No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA3440.11299026772781169No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3430.11266180764720758No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCTCCTCC3340.10970566692177065No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC3320.10904874676056246No Hit
ATATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT3240.10642106611572963No Hit
CATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA3220.10576414595452141No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG3200.1051072257933132No Hit
TAACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTT3190.10477876571270911No Hit
GGAACATATTGTACTAAAAATCAAGCAATGTTTTAGAAAACTGCCTGTAA3180.104450305632105No Hit
GTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCC3160.10379338547089678No Hit
CCCCAAAAGACCGCCGTGTGGTGGGGTGAACCCTGGCCCGAATGCTCCCG3150.1034649253902927No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA3140.10313646530968859No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC3130.10280800522908448No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA3120.10247954514848037No Hit
GGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAAT3100.10182262498727218No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC3100.10182262498727218No Hit
ATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTGAGGTTAGGAT3080.10116570482606396No Hit
GACTTTGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG3080.10116570482606396No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC3070.10083724474545985No Hit
CTATTACCAATTTTCTTTTGTCTCTGGGTATACATTTGAACCCTAATAAA3070.10083724474545985No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG3070.10083724474545985No Hit
CTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCTAAAAC3060.10050878466485574No Hit
GTATAAAGAATTTGGAGCTTCTACGGAGTTACTCTCTTTTTTGCCTTCTG3060.10050878466485574No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)