FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences480620
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19920.41446464982730635No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT19680.40947109982938706No Hit
CTCCAAAAGAGACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA17420.3624485040156465No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA16770.34892430610461483No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA15880.33040655819566395No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT15210.31646623111813904No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA13120.2729807332195913No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAATACAAAGACC11160.23220007490324995No Hit
GATGTAAGCAATTTGTGGGACCCCTTACAGTAAATGAAAACAGGAGACTA11090.2307436228205235No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG10950.2278307186550705No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC10060.2093129707461196No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG9910.20619200199742No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC9710.2020307103324872No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGAGTTTTTGTACAATATGTT9660.200990387416254No Hit
CATTTAAACCCTCATAAAACAAAACGATGGGGATATTCCCTTAACTTCAT9380.1951645790853481No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT9220.19183554575340186No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT9130.1899629645041821No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT8990.18705006033872915No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8980.1868419957554825No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGA8550.177895218675877No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT8500.1768548957596438No Hit
ATGTAATTGGGTGTTGGGGCACATTGCCTCAGGAACATATTGTACAAAAA7710.16041779368315925No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTC7580.15771295410095293No Hit
ATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTTC7470.1554242436852399No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7230.15043069368732054No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGAGACCAAATATTCAA7220.1502226291040739No Hit
GGAACATATTGTACAAAAACTCAAAATGTGTTTTAGGAAACTTCCTGTAA7210.15001456452082726No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC7170.1491823061878407No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG7050.14668553118888103No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA6980.14522907910615454No Hit
ATATGTTCCTGAGGCAATGTGCCCCAACACCCAATTACATATCCCATGAA6910.14377262702342808No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA6900.14356456244018143No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6880.14314843327368815No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTGAAA6810.14169198119096169No Hit
CTGTAAGGGGTCCCACAAATTGCTTACATCTATCCACAATGTCCTCCTGC6700.13940327077524864No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC6680.13898714160875536No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGATTA6650.13836294785901543No Hit
ACCTTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATT6600.13732262494278225No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACCAAATGCC6520.1356581082768091No Hit
GTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGG6490.1350339145270692No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC6490.1350339145270692No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGTCTGGA6360.13232907494486287No Hit
GCTTACATCTATCCACAATGTCCTCCTGCAAATGAATGTCAGGAAACGAA6340.1319129457783696No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6300.13108068744538304No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC6260.13024842911239648No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA6250.13004036452914985No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT6240.1298322999459032No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT6170.12837584786317674No Hit
CCTCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAGACC6160.1281677832799301No Hit
GTCCGGAGGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC6130.12754358953019018No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6050.12587907286421707No Hit
GTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG5980.12442262078149056No Hit
AGTATACATCGTTCCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG5970.12421455619824394No Hit
GAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGGAAGATCCAGC5930.12338229786525738No Hit
GGATAGATGTAAGCAATTTGTGGGACCCCTTACAGTAAATGAAAACAGGA5920.12317423328201073No Hit
GTGTATCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCCCGTC5910.1229661686987641No Hit
CCTGTAAACAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5790.12046939369980442No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT5790.12046939369980442No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCGTTTTGTTTTA5780.12026132911655776No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC5770.12005326453331114No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCGTTTTGTTT5760.11984519995006448No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG5730.11922100620032458No Hit
CAATAGGTCTGTTTACAGGAAGTTTCCTAAAACACATTTTGAGTTTTTGT5710.1188048770338313No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC5670.11797261870084474No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG5600.11651616661811826No Hit
GTGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCGTTGACATACT5580.11610003745162498No Hit
GGTTAGTGAGTGACTGGAGATTTGGGACTGCGAATTTTGGCCAAGACACA5550.11547584370188507No Hit
GCCTAGCAGCCATGGGAACGATGTATACTTGCGGGAGAGCACGACAGAAT5490.11422745620240522No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA5480.11401939161915858No Hit
GCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA5320.11069035828721234No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG5290.11006616453747244No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA5290.11006616453747244No Hit
GTCGATACACCAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGG5280.10985809995422578No Hit
GAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACA5270.10965003537097916No Hit
TATCCACATTGTGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG5250.10923390620448588No Hit
ATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAA5250.10923390620448588No Hit
GGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA5190.10798551870500603No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG5180.10777745412175939No Hit
GTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC5150.10715326037201947No Hit
CTCACTAACCTGTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTC5120.10652906662227954No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT5120.10652906662227954No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5100.10611293745578626No Hit
ACTACATGCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATA5100.10611293745578626No Hit
GACCAGACCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT5070.10548874370604636No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA5020.10444842078981315No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACC5020.10444842078981315No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA4870.10132745204111356No Hit
ATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAACCTTAT4870.10132745204111356No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG4840.10070325829137365No Hit
GTAATTGGGTGTTGGGGCACATTGCCTCAGGAACATATTGTACAAAAACT4830.100495193708127No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)