FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483594
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25890.5353664437524039No Hit
TTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACC15450.31948287199593045No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA14960.3093504055054447No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC13730.28391584676402104No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA13700.2832954916727668No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT13120.2713019599085183No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCCGCTAGG12590.2603423532963602No Hit
CTTTTATATAAGATCCCCGCCTTCCACAGAGTGTGTAAATAATGTCGCGT12260.2535184472925636No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT12110.2504166718362924No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC11670.241318130497897No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG11390.23552814964619084No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT10930.22601603824695923No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA10420.21547000169563724No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA10280.21257501126978415No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG9740.20140861962720794No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9580.1981000591405187No Hit
TTGCTTACATCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAG8960.18527938725459786No Hit
CATTTAAACCCTCACAAAACAAAACGATGGGGATATTCCCTTAACTTCAT8910.18424546210250747No Hit
ATCCCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG8820.18238439682874477No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG8700.17990297646372783No Hit
TGATCAACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGG8380.17328585549034936No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT8250.17059765009491434No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA8220.1699772950036601No Hit
GTCCGGTGCTGGTCGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC8130.1681162297298974No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG7930.16398052912153582No Hit
TCCACAGAGTGTGTAAATAATGTCGCGTCTGAAAGTAATGATTAACTACA7910.16356695906069968No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA7900.1633601740302816No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA7640.15798376323941157No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG7360.1521937823877054No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT7320.15136664226603308No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA7270.1503327171139427No Hit
CATGTATACAAGCAAAACAGGCTTTTTCTTTCTCGCCAACTTACAAGGCC7190.14867843687059806No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT7180.14847165184018No Hit
CATTAGGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT7100.14681737159683536No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG7040.14557666141432687No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC6920.14309524104930996No Hit
CTTCAAAGACTGTGTGTTTAACGAGTGGGAGGAGTTGGGGGAGGAGGTTA6710.13875275541053034No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC6610.13668490510634956No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT6560.13565097995425915No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC6420.13275598952840606No Hit
ATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGA6390.13213563443715182No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT6390.13213563443715182No Hit
GTACAAAGACCTTTAACCTAACCTCCTCCCCCAACTCCTCCCACTCGTTA6360.13151527934589757No Hit
GTGTGTAAATAATGTCGCGTCTGAAAGTAATGATTAACTACATACTCTGG6250.12924064401129873No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA6190.12799993382879027No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG6180.1277931487983722No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA6120.12655243861586374No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA6110.12634565358544564No Hit
GTGTAAATAATGTCGCGTCTGAAAGTAATGATTAACTACATACTCTGGAT6090.1259320835246095No Hit
GTACAATAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG6030.12469137334210102No Hit
AGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACAACTATTGT6000.12407101825084679No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5910.12220995297708409No Hit
GGGTAAAGGGTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA5900.12200316794666602No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG5870.12138281285541178No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT5840.12076245776415755No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGACTTGTGGGTCT5550.11476569188203327No Hit
GTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGAAAA5490.1135249816995248No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCGTTTTGTTT5450.1126978415778525No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5440.11249105654743442No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT5360.11083677630408979No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT5320.11000963618241749No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5310.10980285115199942No Hit
GCTTACATCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAA5250.10856214096949095No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT5200.10752821581740055No Hit
CTATTGTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCCC5190.10732143078698247No Hit
CTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCTGAC5190.10732143078698247No Hit
TGTACGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGAGACTAAA5180.10711464575656439No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT5180.10711464575656439No Hit
ATCCTAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG5110.10566715054363784No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA5060.10463322539154744No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT5050.10442644036112937No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC5050.10442644036112937No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCGTTTTGTTTTG5040.1042196553307113No Hit
TTGACATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTCCTAAAAC4990.1031857301786209No Hit
CTCTTTTATATAAGATCCCCGCCTTCCACAGAGTGTGTAAATAATGTCGC4980.10297894514820283No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGC4970.10277216011778476No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)