FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483594
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25770.532885023387387No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA16150.3339578241251959No Hit
TTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACC15550.3215507223001113No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT14390.2975636587716142No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA13690.28308870664234875No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC12860.26592554911764826No Hit
CTTTTATATAAGATCCCCGCCTTCCACAGAGTGTGTAAATAATGTCGCGT12550.25951521317468784No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG12070.2495895317146201No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCCGCTAGG11810.24421312092375008No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA11520.23821635504162583No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC11290.23346029934201004No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT10600.21919213224316264No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT10540.21795142206065418No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA9810.2028561148401345No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9800.2026493298097164No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT9330.19293043338006674No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG9070.18755402258919673No Hit
ATCCCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG8860.1832115369504171No Hit
CATTTAAACCCTCACAAAACAAAACGATGGGGATATTCCCTTAACTTCAT8770.18135047167665438No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG8430.17431978064243972No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA8210.169770509973242No Hit
TCCACAGAGTGTGTAAATAATGTCGCGTCTGAAAGTAATGATTAACTACA8150.16852979979073354No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT7900.1633601740302816No Hit
TGATCAACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGG7880.16294660396944546No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA7850.16232624887819122No Hit
TTGCTTACATCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAG7700.15922447342192003No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7660.15839733330024772No Hit
GTCCGGTGCTGGTCGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC7460.15426163269188617No Hit
CTTCAAAGACTGTGTGTTTAACGAGTGGGAGGAGTTGGGGGAGGAGGTTA7060.14599023147516305No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA6990.14454273626223652No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG6930.14330202607972803No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA6890.14247488595805574No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG6710.13875275541053034No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC6690.13833918534969417No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC6630.13709847516718568No Hit
CATTAGGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT6630.13709847516718568No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC6620.1368916901367676No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA6530.1350306248630049No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6520.13482383983258683No Hit
GTGTGTAAATAATGTCGCGTCTGAAAGTAATGATTAACTACATACTCTGG6500.1344102697717507No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT6470.13378991468049645No Hit
CATGTATACAAGCAAAACAGGCTTTTTCTTTCTCGCCAACTTACAAGGCC6420.13275598952840606No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT6420.13275598952840606No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG6340.13110170928506143No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC6310.13048135419380721No Hit
GGGTAAAGGGTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA6270.1296542140721349No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT6160.12737957873753603No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT6140.12696600867669988No Hit
GTACAATAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG6000.12407101825084679No Hit
GTGTAAATAATGTCGCGTCTGAAAGTAATGATTAACTACATACTCTGGAT5950.12303709309875639No Hit
AGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACAACTATTGT5810.1201421026729033No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT5810.1201421026729033No Hit
GTACAAAGACCTTTAACCTAACCTCCTCCCCCAACTCCTCCCACTCGTTA5730.11848782242955867No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT5570.11517926194286943No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5560.11497247691245135No Hit
GTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGAAAA5370.11104356133450788No Hit
CTCTTTTATATAAGATCCCCGCCTTCCACAGAGTGTGTAAATAATGTCGC5350.11062999127367172No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA5280.10918249606074518No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT5250.10856214096949095No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG5210.10773500084781862No Hit
ATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGA5140.10628750563489207No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA5140.10628750563489207No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA5100.10546036551321977No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5090.10525358048280167No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA5080.1050467954523836No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGACTTGTGGGTCT5060.10463322539154744No Hit
CTATTGTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCCC5050.10442644036112937No Hit
GTATGGATCGGCAGAGGAGACCCGAAGGTTCCACGCATGCGCTGATGGCC4960.10256537508736667No Hit
GCTTACATCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAA4890.10111787987444013No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA4850.10029073975276782No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT4850.10029073975276782No Hit
ATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCGGGCATAA4840.10008395472234974No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)