FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences582027
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA26930.4626933114786771No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT25930.4455119779666579No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGTTATTCCCTTAACTTCAT20940.3597771237416821No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC19140.32885072342004756No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA18870.32421176337180235No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG17270.2967216297525716No Hit
CTCATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA14230.24449037587603323No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13850.23796146914146593No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT13810.23727421580098518No Hit
CTCCAAAGGACACCAAATATTCAAGCACAGTTTCTCTCCCAAAAGTAAGA13790.2369305891307448No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT12710.21837474893776407No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11420.19621082870725928No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGTCTGGA11410.19603901537213908No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTAAAA11240.19311818867509584No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA11080.19036917531317277No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACACCCAATTACATATCCCATGAA10990.18882285529709103No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTC10890.1871047219458891No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT10800.18555840192980738No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT10490.18023218854108142No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTATAAGTTGGCGAGAAAGTAA10090.17335965513627374No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA10010.1719851484553122No Hit
CAATTAAACCCTCACAAAACAAAAAGATGGGGTTATTCCCTTAACTTCAT9980.17146970844995163No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTATAAGTTGGCGA9740.16734618840706703No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT9640.1656280550558651No Hit
GTTGATAGATGTAAGCAATTTGTTGGGCCCCTTACAGTAAATGAAAACAG9580.16459717504514396No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT9560.16425354837490358No Hit
ATCTTAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG9500.16322266836418242No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC9360.16081728167249973No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT8870.15239842825161032No Hit
CCTGTAAACAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT8670.1489621615492065No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT8570.14724402819800456No Hit
CTATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGT8540.14672858819264398No Hit
ACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACAAAACC8500.14604133485216322No Hit
GATGTAAGCAATTTGTTGGGCCCCTTACAGTAAATGAAAACAGGAGACTA8500.14604133485216322No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG8480.14569770818192285No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG8380.1439795748307209No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG8150.1400278681229565No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC8150.1400278681229565No Hit
CAATAGGTCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT8140.1398560547878363No Hit
GGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGA7910.13590434808007187No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC7890.1355607214098315No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAGGACACCAAATATTCAA7640.13126538803182672No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG7620.13092176136158631No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG7610.13074994802646614No Hit
GTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGG7600.13057813469134594No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGTCTGT7510.1290318146752642No Hit
ATTGTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA7500.128860001340144No Hit
ATGTAATTGGGTGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA7490.12868818800502382No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7480.12851637466990362No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG7460.12817274799966324No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC7410.1273136813240623No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT7270.1249082946323796No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTATAAGGCC7250.12456466796213922No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT7210.12387741462165844No Hit
TAGATAAAGGGATCAAACCTTATTATCCAGAGCATGTAGTTAATCATTAC7200.12370560128653826No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAGGACACCAAATATTCAAGCA7200.12370560128653826No Hit
GCCTAGCAGCCATGGGAAGGATGTATACTTGCGGGAGAGCACGACAGAAT7130.12250290794069692No Hit
ATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAACCTTATTATCCA7030.12078477458949499No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATAACCCCATCTTTTTGTTT6940.11923845457341327No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC6820.11717669455197095No Hit
CCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGGT6820.11717669455197095No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA6790.11666125454661037No Hit
GGAATATGATAAAACGCCGCAGACACATCCAGCGATAGCCAGGACAAATT6740.11580218787100942No Hit
CAATTACATATCCCATGAAGTTAAGGGAATAACCCCATCTTTTTGTTTTG6680.11477130786028827No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGGTT6670.11459949452516807No Hit
CTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCTGAC6620.11374042784956713No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTAAAAGGGGCGGCA6610.11356861451444693No Hit
CATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT6550.11253773450372578No Hit
ACTACATGCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATA6550.11253773450372578No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA6520.1120222944983652No Hit
GTATACATCCTTCCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC6520.1120222944983652No Hit
CTTTTACGCAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA6510.111850481163245No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6500.1116786678281248No Hit
GGATATCCACATTGCGTAAAAGGGGCGGCAAACCCCAAAAGACCCACAAT6440.11064778781740366No Hit
CTTATAAGGCCTTTCTAAGTAAACAGTATCTGAACCTTTACCCCGTTGCT6410.11013234781204308No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC6400.10996053447692289No Hit
TATCCACATTGCGTAAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG6380.10961690780668251No Hit
GCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA6370.10944509447156231No Hit
GGTTCAGATACTGTTTACTTAGAAAGGCCTTATAAGTTGGCGAGAAAGTA6360.10927328113644214No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC6310.10841421446084115No Hit
TGATAGATGTAAGCAATTTGTTGGGCCCCTTACAGTAAATGAAAACAGGA6290.10807058779060078No Hit
CTGTTTACTTAGAAAGGCCTTATAAGTTGGCGAGAAAGTAAAAGCCTGTT6240.1072115211149998No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAGTCTCCTGTTTTCATTTAC6210.10669608110963924No Hit
GTACCATTGGGATAAAACCTAGCAGGCATAATTAATTTTAGTCTCCTGTT6180.10618064110427866No Hit
GGTTTAATTGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA6150.10566520109891808No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT6070.10429069441795656No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG6050.10394706774771616No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG6040.10377525441259598No Hit
GGGACGAACCCTGGCCCGAATGCTCCCACTCCCACCTTGTTGGCGTCCGG6000.10308800107211522No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC5980.10274437440187484No Hit
GCGTAAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCGTTGACATACT5960.10240074773163443No Hit
TTGACATACTTTCCAATCAATAGGTCTGTTTACAGGAAGTTTCCTAAAAC5950.10222893439651425No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG5880.10102624105067291No Hit
GAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACA5850.10051080104531233No Hit
GCTTTAATCCTAAATGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAG5840.10033898771019215No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)