FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences582027
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA26490.4551335247333887No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT26190.4499791246797829No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGTTATTCCCTTAACTTCAT20790.3571999237148792No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC18030.3097794432217062No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG17150.29465986973112934No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA15680.2694033094684611No Hit
CTCCAAAGGACACCAAATATTCAAGCACAGTTTCTCTCCCAAAAGTAAGA15350.26373346940949477No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT14720.25290922929692267No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT14400.24741120257307653No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13780.2367587757956246No Hit
CTCATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA11800.20273973544182658No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11710.20119341542574484No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTAAAA11550.19844440206382177No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT11430.19638264204237948No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA11430.19638264204237948No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACACCCAATTACATATCCCATGAA11390.19569538870189868No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTC10730.18435570858396602No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT10650.1829812019030045No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGTCTGGA10510.1805758152113218No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTATAAGTTGGCGAGAAAGTAA10270.17645229516843722No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTATAAGTTGGCGA10120.17387509514163432No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT10100.17353146847139395No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC10050.172672401795793No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA10050.172672401795793No Hit
ATCTTAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG10040.1725005884606728No Hit
GTTGATAGATGTAAGCAATTTGTTGGGCCCCTTACAGTAAATGAAAACAG9360.16081728167249973No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT9350.16064546833737953No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT9110.15652194829449492No Hit
CAATTAAACCCTCACAAAACAAAAAGATGGGGTTATTCCCTTAACTTCAT9110.15652194829449492No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG8590.14758765486824493No Hit
GGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGA8210.14105874813367764No Hit
CTATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGT8210.14105874813367764No Hit
CAATAGGTCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT8120.13951242811759593No Hit
GTATACATCCTTCCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC8110.13934061478247572No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG8070.13865336144199494No Hit
GTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGG8060.13848154810687477No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT8030.1379661081015142No Hit
ACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACAAAACC7890.1355607214098315No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG7870.13521709473959112No Hit
ATGTAATTGGGTGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA7830.13452984139911034No Hit
CCTGTAAACAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT7750.1331553347181488No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG7570.13006269468598536No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAGGACACCAAATATTCAA7560.12989088135086516No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC7550.12971906801574498No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC7470.12834456133478345No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT7290.12525192130262No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC7250.12456466796213922No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGTCTGT7030.12078477458949499No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG6950.11941026790853344No Hit
GATGTAAGCAATTTGTTGGGCCCCTTACAGTAAATGAAAACAGGAGACTA6930.11906664123829307No Hit
CCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGGT6920.11889482790317288No Hit
CTTTTACGCAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA6920.11889482790317288No Hit
GGTTCAGATACTGTTTACTTAGAAAGGCCTTATAAGTTGGCGAGAAAGTA6850.11769213455733152No Hit
ATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAACCTTATTATCCA6840.11752032122221134No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTATAAGGCC6800.11683306788173058No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA6700.11511493453052865No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT6630.1139122411846873No Hit
GTACCATTGGGATAAAACCTAGCAGGCATAATTAATTTTAGTCTCCTGTT6620.11374042784956713No Hit
GAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACA6620.11374042784956713No Hit
TAGATAAAGGGATCAAACCTTATTATCCAGAGCATGTAGTTAATCATTAC6610.11356861451444693No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC6560.11270954783884596No Hit
GCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA6500.1116786678281248No Hit
ATTGTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA6480.11133504115788442No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC6480.11133504115788442No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC6450.11081960115252386No Hit
ACTACATGCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATA6390.1097887211418027No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA6360.10927328113644214No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC6310.10841421446084115No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG6240.1072115211149998No Hit
CTTATAAGGCCTTTCTAAGTAAACAGTATCTGAACCTTTACCCCGTTGCT6190.10635245443939886No Hit
CTGTTTACTTAGAAAGGCCTTATAAGTTGGCGAGAAAGTAAAAGCCTGTT6160.10583701443403828No Hit
GCCTAGCAGCCATGGGAAGGATGTATACTTGCGGGAGAGCACGACAGAAT6150.10566520109891808No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAGTCTCCTGTTTTCATTTAC6100.10480613442331714No Hit
ACCTTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATT6080.10446250775307674No Hit
CAATTACATATCCCATGAAGTTAAGGGAATAACCCCATCTTTTTGTTTTG6080.10446250775307674No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6070.10429069441795656No Hit
CATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT6060.10411888108283636No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6040.10377525441259598No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG5940.10205712106139406No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTAAAAGGGGCGGCA5900.10136986772091329No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5890.10119805438579309No Hit
GGTTTAATTGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA5890.10119805438579309No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTGCT5880.10102624105067291No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGGTT5860.10068261438043252No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)