FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489378

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489378
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493595
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21350.43254084826629113No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAACCTTAT17190.34826122630901857No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTACATGTTCCGG16910.3425885594465098No Hit
TTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATGTCCTCCTGC11240.22771705548070786No Hit
CTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT10700.2167769122458696No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC10060.20381081656013533No Hit
GTACGGGACCATGCAAGACCTGCACGAGTCCTGCTCAAGGAAACTCTACG9640.19530181626637222No Hit
ATCTCAACCTTTACCCCGTTGCCCGGCAACGGTCTGGTCTCTGCCAAGTG8580.17382672028687488No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA8220.16653329146364937No Hit
CTCTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT7890.15984764837569262No Hit
CTCCAAAAGACACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGA7750.15701131494443826No Hit
GGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG7490.1517438385721087No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACAGACT7330.14850231465067515No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAAC7300.14789452891540636No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7280.14748933842522716No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAA7210.14607117170959996No Hit
GGTTGAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG7110.14404521925870398No Hit
GTATACATGCATACAAAGGCACTAAGGCAGGATAGCCACATTGTGTAAAA6950.14080369533727044No Hit
ATTCTTTGACAGACTTTCCAATCAATAGGTCTATTTACAGGAAGTTTGCG6830.13837255239619525No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA6750.13675179043547847No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC6700.1357388142100305No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT6640.1345232427394929No Hit
CCCTTAGACAAAGGCATTAAACCTTATTATCCGGAACATGTAGTTAATCA6550.13269988553368653No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC6430.13026874259261134No Hit
GCCTAGCAGCCATGGAAATGAGGTGTATTTCCGAGAGAGGACAACAGAGT6280.1272298139162674No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC6190.125406456710461No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAGTGCCTTTGTATGCATGTATA6130.12419088523992342No Hit
ACATATTGTTGACATCTATTGATAATGTCCTCCTGCAAATGAATATGAGG6130.12419088523992342No Hit
GATTTGCCTCTGGCCATTGATCCTTGTTGGGGTTGAAGTCCCAATCTGGA6120.12398828999483381No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6050.12257012327920663No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTACA6040.12236752803411705No Hit
GGCCTACATATTGTTGACATCTATTGATAATGTCCTCCTGCAAATGAATA6030.12216493278902744No Hit
CCCTCATAAAACCAAACGTTGGGGCTACTCCCTTAACTTTATGGGATATG6030.12216493278902744No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGACGATGGGATGGGAATACAAG5750.1164922659265187No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCAAG5730.11608707543633952No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA5700.11547928970107073No Hit
ATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCTGGTCTCTGCCAAGTG5680.11507409921089151No Hit
ACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGA5660.11466890872071232No Hit
CTGTAGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACA5610.11365593249526434No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCATTTCCTCAT5570.11284555151490595No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG5570.11284555151490595No Hit
CCTCTGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT5530.11203517053454755No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCATTTCCTCATATTCATTTGCAGG5330.1079832656327556No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAAGTTAAGGGAG5270.10676769416221803No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5240.10615990842694922No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC5210.10555212269168042No Hit
CCCTTAACTTTATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG5130.10393136073096364No Hit
CTCTCACAATTAATGAAAAAAGAAGATTAAAATTAATTATGCCTGCTAGG5080.10291838450551566No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5020.10170281303497808No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG4970.10068983680953007No Hit
ATATTGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4960.1004872415644405No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC4950.10028464631935088No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)