FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493595
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21310.43173046728593284No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTACATGTTCCGG18250.3697363222885159No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAACCTTAT14860.30105653420314227No Hit
TTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATGTCCTCCTGC11650.2360234605293814No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACAGACT9590.19428884004092425No Hit
GTACGGGACCATGCAAGACCTGCACGAGTCCTGCTCAAGGAAACTCTACG9560.19368105430565546No Hit
ATCTCAACCTTTACCCCGTTGCCCGGCAACGGTCTGGTCTCTGCCAAGTG9320.1888187684235051No Hit
CTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT9160.18557724450207153No Hit
CTCCAAAAGACACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGA8370.16957222013999332No Hit
GTATACATGCATACAAAGGCACTAAGGCAGGATAGCCACATTGTGTAAAA7540.1527568147975567No Hit
GGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG7490.1517438385721087No Hit
CTCTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT7480.1515412433270191No Hit
CCCTCATAAAACCAAACGTTGGGGCTACTCCCTTAACTTTATGGGATATG7460.15113605283683992No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC7200.14586857646451037No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAAC7180.14546338597433117No Hit
GGTTGAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG7170.14526079072924158No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCAAG7070.14323483827834563No Hit
GATTTGCCTCTGGCCATTGATCCTTGTTGGGGTTGAAGTCCCAATCTGGA6910.13999331435691204No Hit
CCCTTAGACAAAGGCATTAAACCTTATTATCCGGAACATGTAGTTAATCA6870.13918293337655366No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6850.13877774288637446No Hit
ATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCTGGTCTCTGCCAAGTG6610.1339154570042241No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC6480.13128171881805933No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT6460.13087652832788013No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6460.13087652832788013No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAA6390.12945836161225296No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG6380.12925576636716338No Hit
GGCCTACATATTGTTGACATCTATTGATAATGTCCTCCTGCAAATGAATA6100.12358309950465463No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAGTGCCTTTGTATGCATGTATA6050.12257012327920663No Hit
ATTCTTTGACAGACTTTCCAATCAATAGGTCTATTTACAGGAAGTTTGCG6000.12155714705375864No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC5990.12135455180866904No Hit
CCCTTAACTTTATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG5790.1173026469068771No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA5740.11628967068142909No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5660.11466890872071232No Hit
GCCTAGCAGCCATGGAAATGAGGTGTATTTCCGAGAGAGGACAACAGAGT5660.11466890872071232No Hit
CTGTAGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACA5630.11406112298544353No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG5580.11304814675999555No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGACGATGGGATGGGAATACAAG5550.11244036102472676No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCATTTCCTCATATTCATTTGCAGG5550.11244036102472676No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTACATGTTCC5510.11162998004436836No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTACA5500.11142738479927876No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5460.11061700381892037No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5460.11061700381892037No Hit
ACATATTGTTGACATCTATTGATAATGTCCTCCTGCAAATGAATATGAGG5400.10940143234838277No Hit
CCTCTGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT5320.107780670387666No Hit
ATATTGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5290.10717288465239722No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAAGTTAAGGGAG5130.10393136073096364No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTTCTTTTTTCA5080.10291838450551566No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCATTTCCTCAT5070.10271578926042606No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC5010.10150021778988848No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4990.10109502729970929No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCATTTC4940.10008205107426128No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)