FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences318593
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT18600.5838169702410285No Hit
CATGTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT17410.5464652393492638No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC14400.45198733179950595No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA13820.43378228649091477No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT12200.3829337116634703No Hit
ACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTT10000.3138800915274347No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA9110.285944763381493No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG8740.2743311999949779No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA8060.25298735377111237No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA7730.242629310750707No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATGTGTT7550.2369794691032132No Hit
CTGCTGAACTCCTAGCCGCTTGTTTTGCTCGCAGCAGGTCTGGGGCAAAA7080.22222710480142377No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA6800.2134384622386556No Hit
CATCATTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGG6760.21218294187254583No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT6450.20245265903519535No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6150.19303625628937232No Hit
GTTCCGCAGTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGC6130.19240849610631747No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT5940.1864447743672962No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA5820.182678213268967No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5710.1792255322621652No Hit
GAAACGATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATG5710.1792255322621652No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT5540.17388957070619881No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT5460.17137852997397934No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA5440.17075076979092446No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA5360.168239729058705No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC5090.15976496658746425No Hit
GTTCAGAAACAGTGAGCCCTGCTCAGAATACTGTCTCTGCCATATCGTCA5080.1594510864959368No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA5030.15788168603829963No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5010.15725392585524478No Hit
CTTCAAAGACTGTGTGTTTAATGAGTGGGAGGAGTTGGGGGAGGAGGTTA4980.15631228558066246No Hit
GGTTTACATGTATACCCAAAGACCAAAGAAAATTGGTAACAGCGGCATAA4980.15631228558066246No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG4960.1556845253976076No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC4960.1556845253976076No Hit
GTTCCTGAACTGGAGCCACCAGCAGGAAAGTACAGGGCCCTGACTCTGGG4840.1519179642992784No Hit
GTATCGAGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG4820.15129020411622354No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC4660.14626812265178457No Hit
ATGTGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA4570.14344320182803766No Hit
ATCCAGCATCCAGGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGC4500.1412460411873456No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4490.14093216109581816No Hit
GCTCTGTATCGAGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA4410.1384211203635987No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG4390.1377933601805438No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC4320.13559619953985177No Hit
ACATATTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTT4300.13496843935679692No Hit
ACTCACAACGGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT4300.13496843935679692No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA4280.13434067917374204No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT4270.13402679908221463No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT4150.1302602379838854No Hit
CCTTACAATAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGGT4140.12994635789235798No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT4120.1293185977093031No Hit
GTGTTGGGTTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG4060.12743531716013848No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC4040.12680755697708362No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4020.12617979679402874No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC4010.1258659167025013No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAATAAATGAAAACAG3980.124924276427919No Hit
TCCTAGTACAAAGACCTTTAACCTAACCTCCTCCCCCAACTCCTCCCACT3940.12366875606180927No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG3920.12304099587875439No Hit
CATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT3920.12304099587875439No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT3900.12241323569569953No Hit
CTGCTGTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCCC3900.12241323569569953No Hit
ATATAAAAGAGAGTCCACACGTACCGCCTCATTTTGCGGGTCACCATATT3870.12147159542111723No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG3850.12084383523806236No Hit
TTATACCGCTGTTACCAATTTTCTTTGGTCTTTGGGTATACATGTAAACC3740.11739115423126058No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC3740.11739115423126058No Hit
GGGTTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGGAAGAT3720.1167633940482057No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG3630.11393847322445878No Hit
GAATATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACACACTCT3620.11362459313293136No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC3580.11236907276682162No Hit
CAGTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTG3560.11174131258376675No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAATAAATGAAAACAGGAGACTA3510.11017191212612959No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG3500.10985803203460215No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG3500.10985803203460215No Hit
GTACAGCAGACGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG3490.10954415194307471No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA3460.10860251166849241No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT3420.10734699130238265No Hit
GTCTACTGTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCC3420.10734699130238265No Hit
GTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTGAG3420.10734699130238265No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT3420.10734699130238265No Hit
GGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGA3410.10703311121085524No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAG3390.10640535102780035No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG3370.10577759084474547No Hit
ATTTGGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA3350.10514983066169062No Hit
CACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAACCT3340.10483595057016318No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA3330.10452207047863575No Hit
GCCTAGCAGCCATGGAAACGATGTATACTTGCGGGAGAGCACGACAGAAT3230.1013832695633614No Hit
GGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA3220.10106938947183398No Hit
CCACAATAGTTGTCTGATTTTTAGGCCCATATTAACGTTGACATAGCTGA3210.10075550938030654No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)