FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences779541
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTCTCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA39550.5073498379174411No Hit
TCCTAGGAGTCCTGATGCGATGTTCTCCATGTTCGGTACAGGGTCCCCAG33450.42909866190489016No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT32600.4181948095096986No Hit
AATTAGAGGACAACAGGTTGGTGAGTGACTGGAGATTTGGGACTGCGAAT32330.4147312328665202No Hit
GGAATATGATAAAACGCCGCAGACACATCCAGCGATAACCAGGACAAATT30950.3970285078013857No Hit
ATTCTATGTTGGGTTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAA30170.3870226197210923No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC29680.3807368695168054No Hit
ATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGA28370.363932108766569No Hit
CTCATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA25210.32339543397973936No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG23330.29927867809390396No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC23240.2981241525461778No Hit
GGAACAGTGAGCCCTGCTCAGAATACTGTCTCTACCATATCGTCAATCTT22720.29145356049264887No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA22650.2905555961777508No Hit
GACTCTGGGATCTTGAAGAGTTTGGTGGAAAGTGGTGGAGTTCCACTGCA21110.270800381249992No Hit
CCTCCAATTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGTTTTATCAT19880.25502186543106775No Hit
TTGTTGGGGTGGAGCCCTCAGGCTCAGGGCCTACTCACAACTGTGCCAGC18640.23911506899572957No Hit
CCCAAAGTCAGGGCCCTGTACCTTCCTGCTGGTGGCTCCAGTTCAGGAAT17570.22538904303942964No Hit
GAGATAAGGGAGTAGGCTGCCTTCCTGACTGCCGATTGGTGGAGGCAGGA17310.22205374701266514No Hit
ATCATCTTCCTCTGCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT16340.2096105272205054No Hit
GTTTCGCCCCAGACCTGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGCA15660.20088744530435218No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG14960.19190780215537093No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAAAACCACATC14920.19139467968971485No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGATTTCTTTCCGTCTGTTCG14870.19075327660764474No Hit
ATCTTAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTG14740.18908562859426253No Hit
GTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGATAA14620.1875462611972943No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA14570.1869048581152242No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG13870.17792521496624297No Hit
CCCAAAGTCAGGGCCCTGTACCTTCCTGCTGGTGGCTCCAGTTCAGGAAC13750.17638584756927475No Hit
GACAATGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAAC13640.17497476078872054No Hit
ATATTAACGTTGACATAACTGACTACTAATTCCCTGGATGCTGGATCTTC13350.171254622912714No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC13230.16971525551574582No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT12930.16586683702332525No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT12650.16227497976373276No Hit
GTCCTGGGAGTCCTGATGCGATGTTCTCCATGTTCGGTACAGGGTCCCCA12490.16022248990110846No Hit
CACTATTCCTGAACTGGAGCCACCAGCAGGAAGGTACAGGGCCCTGACTT12000.1539367396968216No Hit
GGGCTGTACCGCCCGCTTCTCCGCCTGTTGTACCGACCGACCACGGGGCG11720.15034488243722907No Hit
GGAATTAGAGGACAAACGGGCAACATACCTTGATAGTCCAGAAGAACCAA11260.14444397408218426No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT11080.14213492298673194No Hit
TCATCTTCCTCTGCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTT10980.14085211682259177No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA10860.13931274942562355No Hit
AATTGGAGGACAACAGGTTGGTGAGTGACTGGAGATTTGGGACTGCGAAT10840.1390561881927955No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG10830.1389279075763815No Hit
TCCTAACATTCATTTGCAGGAGGACATTGTTGATAGATGTAAGCAATTTG10680.13700369833017123No Hit
GGGCAAAACTCATCGGGACTGACAATTCTGTCGTGCTCTCCCGCAAGTAT10560.135464330933203No Hit
ACTACATACTCTGGATAATAAGGTTTGATCCCTTTCTCTAAGGGCAAATA10540.13520776970037496No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA10470.13430980538547682No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT10440.1339249635362348No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA10370.13302699922133665No Hit
CCTCCCGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA10150.13020482566022826No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT10100.12956342257815817No Hit
GGTTAAGATACTGTTTACTGAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA10080.12930686134533012No Hit
GGGTTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGGAAGAT10070.12917858072891614No Hit
ACATAGAGGTTCCTTGAGCAGGAGTTGTGCAGGTTTTGCATGGTCCGGTG10000.128280616414018No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTAAAA9890.1268695296334638No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT9860.12648468778422173No Hit
CACTATTCCTGAACTGGAGCCACCAGCAGGAAGGTACAGGGCCCTGACTC9810.12584328470215167No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG9780.12545844285290958No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA9760.12520188162008156No Hit
CCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGCCAAG9720.12468875915442548No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG9660.12391907545594139No Hit
GTACAGCCCCAAGCGGCCCCGGGAGGGGTCGTCCGCGGGATTCAGCGCCG9630.12353423360669932No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG9430.12096862127841898No Hit
GCCATTTAGGATTAAAGCAAGGTACCGTAGAAGAATAAAGCCCCGTAAAG9280.11904441203220871No Hit
GGATAATAAGGTTTGATCCCTTTCTCTAAGGGCAAATATTTAGTAACATT9220.11827472833372459No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT9220.11827472833372459No Hit
CATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT8980.11519599353978814No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT8780.1126303812115078No Hit
GCGTAAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCGTTGACATACT8660.11109101381453959No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG8610.11044961073246949No Hit
GCTCAGAATACTGTCTCTACCATATCGTCAATCTTATCGAAGACTGGGGA8580.11006476888322744No Hit
ACACAGAATAGCTTGCCTGAGTGCCGTATGGTGAGGTGAACAATGTTCCG8500.1090385239519153No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT8380.10749915655494707No Hit
CCCCATCAATCCCCCATGGGGAGGGGTGAACCCTGGCCCGAATGCTCCCA8280.1062163503908069No Hit
GGAATTAGAGGACAAACGGGCAACATACCTTGACAGTCCAGAAGAACCAA8130.10429214114459663No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8120.1041638605281826No Hit
GTACAACAGGCGGAGAAGCGGGCGGTACAGCCCCAAGCGGCCCCGGGAGG8120.1041638605281826No Hit
GGATATCCACATTGCGTAAAAGGGGCGGCAAACCCCAAAAGACCCACAAT8100.10390729929535458No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG8090.10377901867894056No Hit
GACTTTGGGATCTTGAAGAGTTTGGTGGAAAGTGGTGGAGTTCCACTGCA8020.10288105436404243No Hit
GTCGTTGACATTGCTGAAAGTCCAAGAGTTCTCTTATGCAAGACCTTGGG7970.10223965128197234No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA7970.10223965128197234No Hit
CCACAATAGTTGTCTGATTTTTAGGCCCATATTAACGTTGACATAACTGA7940.10185480943273029No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG7920.10159824819990224No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA7810.10018716141934805No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA7810.10018716141934805No Hit
GTGTAAGTAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT7810.10018716141934805No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)