FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences779541
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTCTCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA43430.5571227170860802No Hit
TCCTAGGAGTCCTGATGCGATGTTCTCCATGTTCGGTACAGGGTCCCCAG31240.4007486456773922No Hit
ATTCTATGTTGGGTTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAA27820.3568766748637981No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC26970.34597282246860656No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT25700.32968118418402625No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG24340.3122350203517198No Hit
GGAATATGATAAAACGCCGCAGACACATCCAGCGATAACCAGGACAAATT24280.31146533665323567No Hit
AATTAGAGGACAACAGGTTGGTGAGTGACTGGAGATTTGGGACTGCGAAT24190.3103108111055095No Hit
ATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGA23780.3050513058325348No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA22490.2885031063151265No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC20060.2573309165265201No Hit
GAGATAAGGGAGTAGGCTGCCTTCCTGACTGCCGATTGGTGGAGGCAGGA20050.25720263591010606No Hit
CTCATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA20000.256561232828036No Hit
GGAACAGTGAGCCCTGCTCAGAATACTGTCTCTACCATATCGTCAATCTT19900.25527842666389583No Hit
ATCATCTTCCTCTGCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT18720.24014131392704166No Hit
GACTCTGGGATCTTGAAGAGTTTGGTGGAAAGTGGTGGAGTTCCACTGCA18280.23449696680482487No Hit
CCTCCAATTTGTCCTGGTTATCGCTGGATGTGTCTGCGGCGTTTTATCAT18170.23308588002427072No Hit
ATCTTAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTG17100.2193598540679708No Hit
TTGTTGGGGTGGAGCCCTCAGGCTCAGGGCCTACTCACAACTGTGCCAGC16380.21012364968616146No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG15460.1983218329760718No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAAAACCACATC15060.1931906083195111No Hit
CCCAAAGTCAGGGCCCTGTACCTTCCTGCTGGTGGCTCCAGTTCAGGAAT14720.18882906736143448No Hit
TCATCTTCCTCTGCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTT14600.18728969996446626No Hit
GTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGATAA14550.18664829688239618No Hit
GTTTCGCCCCAGACCTGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGCA14430.18510892948542795No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA14400.18472408763618592No Hit
GGGCTGTACCGCCCGCTTCTCCGCCTGTTGTACCGACCGACCACGGGGCG13730.1761292863364467No Hit
GTCCTGGGAGTCCTGATGCGATGTTCTCCATGTTCGGTACAGGGTCCCCA13510.1733071127753383No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGATTTCTTTCCGTCTGTTCG12860.16496887270842714No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG12620.16189013791449072No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT12300.15778515818924213No Hit
ATATTAACGTTGACATAACTGACTACTAATTCCCTGGATGCTGGATCTTC12080.15496298462813374No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA11680.149831759971573No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA11430.14662474456122257No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA11180.14341772915087211No Hit
CCCAAAGTCAGGGCCCTGTACCTTCCTGCTGGTGGCTCCAGTTCAGGAAC10970.14072383620617773No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG10760.13802994326148338No Hit
GGTTAAGATACTGTTTACTGAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA10740.13777338202865533No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTAAAA10570.13559261154961702No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT10380.1331552798377507No Hit
GACAATGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAAC10370.13302699922133665No Hit
TCCTAACATTCATTTGCAGGAGGACATTGTTGATAGATGTAAGCAATTTG10240.13135935120795442No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC10200.13084622874229834No Hit
GTACAGCCCCAAGCGGCCCCGGGAGGGGTCGTCCGCGGGATTCAGCGCCG10030.12866545826326006No Hit
CCTCCCGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA9950.12763921333194791No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG9900.1269978102498778No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA9810.12584328470215167No Hit
CACTATTCCTGAACTGGAGCCACCAGCAGGAAGGTACAGGGCCCTGACTT9770.1253301622364956No Hit
GGGCAAAACTCATCGGGACTGACAATTCTGTCGTGCTCTCCCGCAAGTAT9700.12443219792159745No Hit
GGGTTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGGAAGAT9440.12109690189483298No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT9220.11827472833372459No Hit
ACTACATACTCTGGATAATAAGGTTTGATCCCTTTCTCTAAGGGCAAATA9080.11647879970392834No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT8890.114041467992062No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG8840.1134000649099919No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT8800.11288694244433584No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA8740.11211725874585173No Hit
AATTGGAGGACAACAGGTTGGTGAGTGACTGGAGATTTGGGACTGCGAAT8700.11160413628019565No Hit
GTCTTCGATAAGATTGACGATATGGTAGAGACAGTATTCTGAGCAGGGCT8590.11019304949964145No Hit
GGATAATAAGGTTTGATCCCTTTCTCTAAGGGCAAATATTTAGTAACATT8550.1096799270339854No Hit
GGAATTAGAGGACAAACGGGCAACATACCTTGATAGTCCAGAAGAACCAA8500.1090385239519153No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG8360.10724259532211905No Hit
GTGTAAGTAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT8290.10634463100722093No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT8250.10583150854156485No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG8220.10544666669232278No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT8200.10519010545949475No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG8140.10442042176101066No Hit
GCTCAGAATACTGTCTCTACCATATCGTCAATCTTATCGAAGACTGGGGA8050.10326589621328448No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8050.10326589621328448No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT8040.10313761559687046No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA8020.10288105436404243No Hit
CCCCATCAATCCCCCATGGGGAGGGGTGAACCCTGGCCCGAATGCTCCCA8010.10275277374762841No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7970.10223965128197234No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)