FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517105
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT25670.4964175554287814No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA22740.43975594898521575No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA17500.3384225640827298No Hit
ATGCAGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGA14900.2881426402761528No Hit
GGATAATAAGATTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT14190.2744123533905106No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG13280.25681438005820867No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT12390.2396031753705727No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA11970.23148103383258722No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG10680.20653445625163167No Hit
ATATGTTCCTGAGGCAATGTACCCCAACTCCCAATTACATATCCCATGAA10480.2026667698049719No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT10260.19841231471364618No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTC10180.19686524013498227No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9990.19319093801065548No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG9820.18990340453099466No Hit
GTTTACAGGAAGTTTTCTAAAACACATTTTGATTTTTTGTACAATATGTT9810.18971002020866168No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC9720.1879695613076648No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT9620.1860357180843349No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT9380.18139449434834318No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGTCTGGA9160.17714003925701743No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA8820.17056497229769582No Hit
GTTGTGAGTATGCCCTGAGCCTGCGGGCTCCACCCCAACAGTCCCCCATG8760.1694046663636979No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC8730.16882451339669893No Hit
GTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGG8710.16843774475203296No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC8280.16012221889171446No Hit
GTATACATGCATATAAAGGCATTAGAGCAGGATATCCACATTGCGTGAAA8050.1556743794780557No Hit
CAATAGGCCTGTTTACAGGAAGTTTTCTAAAACACATTTTGATTTTTTGT7980.1543206892217248No Hit
GTGGATATCCTGCTCTAATGCCTTTATATGCATGTATACAAGCAAAACAG7900.15277361464306088No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT7820.15122654006439698No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7460.1442647044604094No Hit
GTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC7240.14001024936908366No Hit
GAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACA7040.13614256292242388No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC6980.13498225698842595No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT6960.13459548834375998No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG6890.13324179808742903No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAT6820.1318881078310981No Hit
ATCTTAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG6800.13150133918643217No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG6750.13053441757476722No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT6610.12782703706210538No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT6600.1276336527397724No Hit
CTTTATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGC6460.12492627222711054No Hit
TCCTCAGGCCATGCAGTGGAACTCAACCACTTTCCACCAAACTCTTCAAG6450.12473288790477756No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACC6340.12260566035911467No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT6300.12183212306978272No Hit
ATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAATCTTAT6180.11951151120178688No Hit
ACTACATGCTCTGGATAATAAGATTTGATCCCTTTATCTAAGGGCAAATA6110.11815782094545596No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT6100.11796443662312296No Hit
GCTCTGGATAATAAGATTTGATCCCTTTATCTAAGGGCAAATATTTAGTA6020.11641736204445906No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC5970.11545044043279411No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGAAAACTTCCTGTAA5880.11370998153179722No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG5850.11312982856479825No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5840.11293644424246525No Hit
GGATATCCACATTGCGTGAAAGGGGCTGCAAACCCCAAAAGACCCACAAG5810.11235629127546629No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGAGACTA5790.11196952263080032No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5750.11119598534146835No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5580.10790845186180757No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5550.1073282988948086No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG5520.10674814592780964No Hit
TAGATAAAGGGATCAAATCTTATTATCCAGAGCATGTAGTTAATCATTAC5470.10578122431614469No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG5450.10539445567147872No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG5410.10462091838214675No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCAACCACTTT5390.10423414973748078No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC5370.1038473810928148No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA5370.1038473810928148No Hit
ATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGCATAA5250.10152676922481893No Hit
GTCTTTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGGGATATT5210.10075323193548698No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)