FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences317335
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG13190.4156490774733326No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA10210.3217420076575228No Hit
ATATTGTATTGGGGGCCAAGTCTGTACAGCATCGTGAGTCCCTTTATACC9670.3047252903083492No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGGAAGGAAGGAGTTT9620.30314966833157386No Hit
ATACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTG8690.27384309956355274No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG8640.2722674775867774No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT8180.25777175540044434No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG8110.2555658846329589No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7980.25146926749334303No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA7750.24422140640017648No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT7550.23791891849307517No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6970.2196417035624813No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT6880.21680558400428568No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC6570.20703672774827864No Hit
TATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT6560.20672160335292356No Hit
AGTCTGTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT6530.20577623016685836No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6460.2035703593993729No Hit
CACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC6280.1978981202829817No Hit
TTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAG6050.19065025918981518No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT6010.1893897616083949No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCGTGAGTCCCTTTATACC5840.1840326468873588No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC5820.18340239809664866No Hit
ATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATATT5510.1736335418406416No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA5400.17016717349173585No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA5160.16260418800321425No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTCAG5140.16197393921250414No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC5130.16165881481714905No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT5110.16102856602643892No Hit
GTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA5100.16071344163108386No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGGGT5060.1594529440496636No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTAT4940.1556714513054028No Hit
ATATGCTCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCCATGAA4920.15504120251469267No Hit
GTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG4920.15504120251469267No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC4900.15441095372398253No Hit
GGTCCTAGGAATCCTGATGTGATGTTCTCCATGTTCGTCACAGGGTCCCC4900.15441095372398253No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC4880.1537807049332724No Hit
GGTCTTAGGAATCCTGATGTGATGTTCTCCATGTTCGTCACAGGGTCCCC4770.1503143365843667No Hit
CTCCTAGACACCGCCTCAGCTCTGTATCGAGAAGCCTTAGAGTCTCCTGA4740.14936896339830147No Hit
GGGTAAAGGTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA4730.14905383900294641No Hit
GTACGAGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG4700.14810846581688122No Hit
GGAGCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA4630.14590259504939576No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTCCGCGAGAGGACGACAGAAT4600.14495722186333054No Hit
GTCTAAGGGCAAATATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAA4550.14338159988655522No Hit
ATACAATATCATCAAGATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTG4510.14212110230513497No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT4270.13455811681661337No Hit
CTCCGAAAGAGACCAAATATTCAAGTACAGTCTCTCTTCCAAAAGTAAGG4260.1342429924212583No Hit
CTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC4240.13361274363054818No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4190.13203712165377285No Hit
CTTTCGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC4160.13109174846770763No Hit
CATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACT4140.1304614996769975No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC4130.13014637528164244No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG4110.1295161264909323No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG4040.12731025572344684No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT4030.12699513132809176No Hit
ATTCTATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAAC4020.1266800069327367No Hit
GTACAATATGCTCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCC4020.1266800069327367No Hit
TTCAAGTACAGTCTCTCTTCCAAAAGTAAGGCAAGATATATGAAACCACA4000.12604975814202657No Hit
CCCATAGGTATTTTGCGAAAGCCCAGGACGATGGGATGGGAATACAGGTG3950.12447413616525124No Hit
TTCTAAAACAGTGTTTGATCTTTTGTACAATATGCTCCTGTGGCAATGTT3920.12352876297918604No Hit
CCCAAAGTCAGGGGTCTGTATTTTCCTGCTGGTGGCTCCAGTTCAGGAAC3880.12226826539776578No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGAAAATACAGACCCCT3880.12226826539776578No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC3860.12163801660705564No Hit
TCCTCAGGCCATGCAGTGGAATTCCACTGCCTTCCACCAAGCTCTGCAGA3810.12006239463028032No Hit
CAGTAAACCCTGCTCCGAATATTGCCTCTCACATCTCGTCAATCTCCGCG3780.1191170214442151No Hit
GTCTAGGAGATCTCTGACGGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA3750.11817164825814991No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTGTTTGATCTTTTGT3740.11785652386279485No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGCCT3730.11754139946743977No Hit
CTTTAATCCTGAATGGCAAACTCCTTCCTTCCCTAAGATTCATTTACAAG3670.11565065309530936No Hit
GTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAACAG3660.11533552869995431No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT3530.11123891156033844No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG3500.11029353837427325No Hit
GTTTTAGAAAACTTCCTGTTAACAGGCCTATTGATTGGAAAGTATGTCAA3470.10934816518820806No Hit
CCCTTAGACAAAGGAATTAAACCTTATTATCCAGATCAGGTAGTTAATCA3470.10934816518820806No Hit
ATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGG3470.10934816518820806No Hit
ATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAAC3450.10871791639749792No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCCTCTACAG3450.10871791639749792No Hit
GGGATCTAGTAGTCAATTATGTTAATACTAACATGGGTTTAAAGATCAGG3440.10840279200214284No Hit
ATGTATATTTCCGCGAGAGGACGACAGAATTGTCAGTTCCGATGAGCTTT3430.10808766760678779No Hit
GTATATTTCCGCGAGAGGACGACAGAATTGTCAGTTCCGATGAGCTTTGC3430.10808766760678779No Hit
ATCTTGATGATATTGTATTGGGGGCCAAGTCTGTACAGCATCGTGAGTCC3430.10808766760678779No Hit
ATTGACCACAGTGTCAACAATTCCTCCTCCTGCCTCCACCAATCGGCAGT3420.1077725432114327No Hit
GGCCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGGGCTTT3390.10682717002536751No Hit
CTACAGCACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTCCCTAAG3390.10682717002536751No Hit
GGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCA3330.1049364236532371No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC3320.10462129925788205No Hit
GGATATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAAT3320.10462129925788205No Hit
GGCTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATATTCAAGTA3300.10399105046717191No Hit
CTCTTTGGAAGGCTGGTATTCTATATAAGAGGGAAACCACACGTAGCGCA3290.10367592607181686No Hit
CTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTT3290.10367592607181686No Hit
ATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTAT3280.10336080167646178No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTGCACTCAGGCA3260.10273055288575164No Hit
ATACAATATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTG3260.10273055288575164No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCCTCTACAGCACCTATC3210.10115493090897631No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG3200.10083980651362126No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)