FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences317335
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAGAG12260.3863425087053114No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10090.317960514913262No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG9820.3094521562386752No Hit
ATATTGTATTGGGGGCCAAGTCTGTACAGCATCGTGAGTCCCTTTATACC9330.294011060866277No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA9070.28581782658704524No Hit
CCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGGAAGGAAGGAGTTT8770.2763640947263933No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT8250.2599776261679298No Hit
CTATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATA7930.24989364551656768No Hit
ATACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTG7710.2429609088187562No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG7680.24201553563269101No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT7390.23287692816739408No Hit
CTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGTTT7380.232561803772039No Hit
TATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCT6910.21775095719035087No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6900.21743583279499584No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6440.2029401106086628No Hit
TTATTAATAGGTGTCAACAATTTGTGGGCCCTCTCACTGTAAATGAAAAG6410.20199473742259755No Hit
AGTCTGTACAGCATCGTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT6390.20136448863188744No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCAT6320.19915861786440198No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC6200.19537712512014116No Hit
ATATAAGAGGGAAACCACACGTAGCGCATCATTTTGCGGGTCACCATATT6080.19159563237588037No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTCTGGGTATACATTTAA5950.1874990152362645No Hit
CACTAAATATTTGCCCTTAGACAAAGGAATTAAACCTTATTATCCAGATC5880.18529314446877904No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCGTGAGTCCCTTTATACC5860.1846628956780689No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCCGTCAG5630.17741503458490238No Hit
GGATAATAAGGTTTAATTCCTTTGTCTAAGGGCAAATATTTAGTGTGGGT5520.17394866623599664No Hit
GTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA5320.16764617832889533No Hit
GTACATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG5260.16575543195676493No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5140.16197393921250414No Hit
GGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTATTCCCTAA5090.16039831723572878No Hit
GTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACCTGATCTGG5020.15819244646824332No Hit
GTCTAAGGGCAAATATTTAGTGTGGGTAGGATAGAATCTAGCAGGCATAA4980.15693194888682307No Hit
CTCTTATATAGAATACCAGCCTTCCAAAGAGTATGTAAATAATGTCTGGT4980.15693194888682307No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTAT4820.151889958561142No Hit
GGTCTTAGGAATCCTGATGTGATGTTCTCCATGTTCGTCACAGGGTCCCC4820.151889958561142No Hit
GGGTAAAGGTTCATGTACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA4800.15125970977043188No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC4750.14968408779365655No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT4720.14873871460759136No Hit
GGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCATTTAC4670.147163092630816No Hit
ATATGCTCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCCATGAA4660.14684796823546095No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC4600.14495722186333054No Hit
GGTCCTAGGAATCCTGATGTGATGTTCTCCATGTTCGTCACAGGGTCCCC4590.1446420974679755No Hit
TTCAAGTACAGTCTCTCTTCCAAAAGTAAGGCAAGATATATGAAACCACA4330.13644886318874377No Hit
TTCTAAAACAGTGTTTGATCTTTTGTACAATATGCTCCTGTGGCAATGTT4290.1351883656073235No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4280.13487324121196842No Hit
CTCCTAGACACCGCCTCAGCTCTGTATCGAGAAGCCTTAGAGTCTCCTGA4280.13487324121196842No Hit
CTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC4260.1342429924212583No Hit
GTACGAGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG4200.1323522460491279No Hit
ATACAATATCATCAAGATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTG4170.1314068728630627No Hit
GGAGCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA4130.13014637528164244No Hit
TCCTCAGGCCATGCAGTGGAATTCCACTGCCTTCCACCAAGCTCTGCAGA4100.12920100209557725No Hit
CTCCGAAAGAGACCAAATATTCAAGTACAGTCTCTCTTCCAAAAGTAAGG4100.12920100209557725No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTCCGCGAGAGGACGACAGAAT4030.12699513132809176No Hit
TCCAAAGAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTACC4030.12699513132809176No Hit
GTTTTAGAAAACTTCCTGTTAACAGGCCTATTGATTGGAAAGTATGTCAA4000.12604975814202657No Hit
ATTCTATCCTACCCACACTAAATATTTGCCCTTAGACAAAGGAATTAAAC4000.12604975814202657No Hit
GTACAATATGCTCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCC3990.12573463374667151No Hit
CCCATAGGTATTTTGCGAAAGCCCAGGACGATGGGATGGGAATACAGGTG3950.12447413616525124No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT3950.12447413616525124No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCCTCTACAG3940.12415901176989616No Hit
GGGATCTAGTAGTCAATTATGTTAATACTAACATGGGTTTAAAGATCAGG3860.12163801660705564No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG3860.12163801660705564No Hit
CTTTCGGAGTGTGGATTCGCACTCCTCCAGCCTATAGACCACCAAATGCC3850.12132289221170056No Hit
CATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACT3800.11974727023492523No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGAAAATACAGACCCCT3730.11754139946743977No Hit
CCCTTAGACAAAGGAATTAAACCTTATTATCCAGATCAGGTAGTTAATCA3700.11659602628137458No Hit
ATCTTGATGATATTGTATTGGGGGCCAAGTCTGTACAGCATCGTGAGTCC3690.1162809018860195No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTGTTTGATCTTTTGT3640.11470527990924417No Hit
CAGTAAACCCTGCTCCGAATATTGCCTCTCACATCTCGTCAATCTCCGCG3630.11439015551388912No Hit
GTCTAGGAGATCTCTGACGGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA3550.11186916035104857No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC3530.11123891156033844No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGCTCCGA3520.11092378716498338No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA3520.11092378716498338No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT3500.11029353837427325No Hit
CCCAAAGTCAGGGGTCTGTATTTTCCTGCTGGTGGCTCCAGTTCAGGAAC3500.11029353837427325No Hit
CTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTT3500.11029353837427325No Hit
CTTTAATCCTGAATGGCAAACTCCTTCCTTCCCTAAGATTCATTTACAAG3500.11029353837427325No Hit
CTCTTTGGAAGGCTGGTATTCTATATAAGAGGGAAACCACACGTAGCGCA3440.10840279200214284No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGCCT3410.10745741881607765No Hit
ATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTAACAGG3400.1071422944207226No Hit
CTTTAAACCCATGTTAGTATTAACATAATTGACTACTAGATCCCTGGATG3390.10682717002536751No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG3390.10682717002536751No Hit
GTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAACAG3370.10619692123465738No Hit
CTACAGCACCTATCTTTAATCCTGAATGGCAAACTCCTTCCTTCCCTAAG3370.10619692123465738No Hit
GGGTAGGATAGAATCTAGCAGGCATAATTAATTTCAATCTTCTCTTTTCA3370.10619692123465738No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACTGCACTCAGGCA3350.10556667244394724No Hit
GTGTTTGATCTTTTGTACAATATGCTCCTGTGGCAATGTTCCCCAACTTC3300.10399105046717191No Hit
ATTGACCACAGTGTCAACAATTCCTCCTCCTGCCTCCACCAATCGGCAGT3290.10367592607181686No Hit
ATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAAC3270.10304567728110672No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC3260.10273055288575164No Hit
GGATATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAAT3250.10241542849039659No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC3250.10241542849039659No Hit
CTCTATGTCTCCCTCATGTTGCTGTACAAAACCTACGGATGGAAATTGCA3240.1021003040950415No Hit
GGCTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATATTCAAGTA3200.10083980651362126No Hit
GTATATTTCCGCGAGAGGACGACAGAATTGTCAGTTCCGATGAGCTTTGC3200.10083980651362126No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)