FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489388

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489388
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences523723
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28700.5479996104811131No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAA27510.525277675412384No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC24510.4679954861634872No Hit
AATATACACTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG14760.28182837110457243No Hit
ATGTAAGACCTTGGGCAAGAATTGGCGGGCGTTCACGGTGGTTTCCATGC14660.2799189647962759No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA12860.24554965124693778No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGCGGGCGTTC12560.23982143232204814No Hit
CACTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT12090.23084722267305424No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC11450.21862702229995626No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT11320.21614479409917073No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT11270.21519009094502248No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT10990.20984375328179208No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10230.19533226533873824No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT9940.1897949870446782No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9790.18693087758223337No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT9770.18654899632057403No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG9630.18387582748895887No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT9480.18101171802651403No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAGAACCTAGCAGG9170.17509255847079466No Hit
GGATAATAGGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATT9050.1728012709008388No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAGAAC8880.16955528017673466No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT8450.16134483305105943No Hit
ATCCAAATGTTACCAAATATTTGCCATTAGATAAGGGTATTAAACCCTAT8290.15828978295778492No Hit
GTCCGGCAGATGAAAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC8180.15618943601865873No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA8050.1537072078178732No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA8030.15332532655621386No Hit
GCCTAGCAGCCATGGAAATGATGTATATTTGCGGGAGAGAACAACAGAGT8020.15313438592538423No Hit
TCCTAGTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGT7850.14988839520128008No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG7740.14778804826215383No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7720.14740616700049455No Hit
ATATTAGTGTTGACATAACTGACTACCAGGTCCCTAGATGCTGGATCTTC7650.14606958258468694No Hit
AGATAAGGGTATTAAACCCTATTATCCAGAACATTTAGTTAATCATTACT7640.14587864195385727No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAATGTTCTGGAT7570.1445420575380497No Hit
CATTTAAATCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT7510.14339641375307174No Hit
CACTAATATGGGCCTGAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT7460.1424417105989235No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAAAAAGGCCTTGTAAGTTGGCGA7380.14091418555228621No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAATCTAATCTCCTCC7330.13995948239813796No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA7310.13957760113647863No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG7270.13881383861316002No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT7240.13824101672067104No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAAAAGGCACAG7040.13442220410407793No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6800.12983962896416618No Hit
CCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG6780.12945774770250687No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT6750.1288849258100179No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT6690.12773928202503995No Hit
CCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAAGAGTCCTCTTATGT6530.12468423193176545No Hit
CATTTGGATAGAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTCTCA6480.12372952877761718No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTATATGCATGTATT6340.12105635994600199No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA6310.12048353805351301No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6310.12048353805351301No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC6260.11952883489936475No Hit
ACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGG6200.1183831911143868No Hit
CCTCTGCACGTCGCATGGAAACCACCGTGAACGCCCGCCAATTCTTGCCC6160.11761942859106818No Hit
CATATAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAGCA6080.11609190354443093No Hit
GTATTAAACCCTATTATCCAGAACATTTAGTTAATCATTACTTCCAAACC6020.11494625975945301No Hit
CTTCAAAGACTGTTTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA5820.11112744714285987No Hit
TCGCAGAAGATCTCAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACT5790.1105546252503709No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT5750.10979086272705227No Hit
CTCTAAGGCATCCCGATACAGAGCTGAGGCGGTATCTAGAAGATCTCGTA5740.10959992209622263No Hit
ACCTTGAGGCATACTTCAAAGACTGTTTGTTTAAAGACTGGGAGGAGTTG5730.10940898146539298No Hit
GTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA5700.108836159572904No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTGATTTTGTGT5700.108836159572904No Hit
GACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAATCTAATCT5680.10845427831124468No Hit
CTCTTATGTAAGACCTTGGGCAAGAATTGGCGGGCGTTCACGGTGGTTTC5660.10807239704958536No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTTTCATCTGCCGGACCGTGTG5630.10749957515709642No Hit
CTCCCCGTCTGTGCCTTTTCATCTGCCGGACCGTGTGCACTTCGCTTCAC5480.10463546569465157No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG5470.10444452506382192No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC5420.10348982190967362No Hit
GGATAGAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTCTCATTGAC5370.10253511875552536No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5340.10196229686303639No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACACTTACA5330.1017713562322067No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG5320.10158041560137707No Hit
CTATTATCCAGAACATTTAGTTAATCATTACTTCCAAACCAGACATTATT5270.10062571244722879No Hit
GTGCACACGGTCCGGCAGATGAAAAGGCACAGACGGGGAGTCCGCGTAAA5260.10043477181639912No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)