FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences523723
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAA30280.5781682301521988No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28580.5457083229111572No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC23910.4565390483137078No Hit
AATATACACTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG12670.2419217792611743No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAGAAC12120.23142004456554324No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC12030.22970157888807632No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA11940.22798311321060943No Hit
GGATAATAGGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATT11380.21729043788414867No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGCGGGCGTTC10950.20907999075847347No Hit
ATGTAAGACCTTGGGCAAGAATTGGCGGGCGTTCACGGTGGTTTCCATGC10840.20697964381934725No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAGAACCTAGCAGG10840.20697964381934725No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT10820.20659776255768794No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT10730.20487929688022102No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG10680.20392459372607277No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT10110.19304097776878235No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9770.18654899632057403No Hit
CACTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT9630.18387582748895887No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT9560.18253924307315125No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT9440.1802479555031954No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT9280.1771929054099209No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9260.17681102414826158No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAATGTTCTGGAT8940.17070092396171258No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG8550.16325423935935598No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC8460.1615357736818891No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA8340.15924448611193323No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA8210.1567622579111477No Hit
AGATAAGGGTATTAAACCCTATTATCCAGAACATTTAGTTAATCATTACT8090.1544709703411918No Hit
TCCTAGTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGT8040.15351626718704353No Hit
ATCCAAATGTTACCAAATATTTGCCATTAGATAAGGGTATTAAACCCTAT8020.15313438592538423No Hit
CTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAAACC7860.15007933583210972No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA7820.1493155733087911No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA7510.14339641375307174No Hit
GTATTAAACCCTATTATCCAGAACATTTAGTTAATCATTACTTCCAAACC7390.14110512618311588No Hit
CACTAATATGGGCCTGAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT7260.13862289798233035No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAAAAAGGCCTTGTAAGTTGGCGA7210.13766819482818207No Hit
GCCTAGCAGCCATGGAAATGATGTATATTTGCGGGAGAGAACAACAGAGT7150.13652255104320413No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT7010.13384938221158896No Hit
ATATTAGTGTTGACATAACTGACTACCAGGTCCCTAGATGCTGGATCTTC6960.13289467905744065No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT6890.13155809464163307No Hit
CATTTAAATCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT6770.1292668070716772No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6770.1292668070716772No Hit
CATTTGGATAGAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTCTCA6730.12850304454835856No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTATATGCATGTATT6690.12773928202503995No Hit
CCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG6670.12735740076338065No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT6620.12640269760923237No Hit
CTCCCCGTCTGTGCCTTTTCATCTGCCGGACCGTGTGCACTTCGCTTCAC6570.12544799445508406No Hit
GTCCGGCAGATGAAAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC6490.12392046940844684No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT6490.12392046940844684No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAATCTAATCTCCTCC6400.12220200373097992No Hit
CTTCAAAGACTGTTTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA6370.12162918183849095No Hit
CCTCTGCACGTCGCATGGAAACCACCGTGAACGCCCGCCAATTCTTGCCC6310.12048353805351301No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC6070.11590096291360127No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC6040.1153281410211123No Hit
CATATAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAGCA5830.11131838777368953No Hit
CTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTTTTGCCTTCT5780.11036368461954124No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG5780.11036368461954124No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG5770.11017274398871159No Hit
CTATTATCCAGAACATTTAGTTAATCATTACTTCCAAACCAGACATTATT5740.10959992209622263No Hit
GACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAATCTAATCT5710.10902710020373366No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACACTTACA5670.10826333768041503No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5670.10826333768041503No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA5600.10692675326460745No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTGATTTTGTGT5580.10654487200294813No Hit
TTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAGAACCT5520.10539922821797018No Hit
GTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA5500.10501734695631089No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT5490.10482640632548121No Hit
CCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAAGAGTCCTCTTATGT5430.10368076254050328No Hit
ACCTTGAGGCATACTTCAAAGACTGTTTGTTTAAAGACTGGGAGGAGTTG5410.10329888127884397No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTTTCATCTGCCGGACCGTGTG5390.10291700001718465No Hit
ACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGG5380.102726059386355No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT5380.102726059386355No Hit
GGATAGAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTCTCATTGAC5350.10215323749386604No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAAAAGGCACAG5340.10196229686303639No Hit
GTTCTGGATAATAGGGTTTAATACCCTTATCTAATGGCAAATATTTGGTA5320.10158041560137707No Hit
GTCTTGGTGTAAGTGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGT5240.10005289055473983No Hit
TCGCAGAAGATCTCAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACT5240.10005289055473983No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)